LOCUS       sequence-48337-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Chromosome- and spindle-pole-derived signals generate an intrinsic 
            code for spindle position and orientation.
  JOURNAL   Nat Cell Biol. 2012 Feb 12;14(3):311-7. doi: 10.1038/ncb2440.
  PUBMED    22327364
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-02-12- 12"; volume: "14"; issue: "3"; 
            pages: "311-7"
COMMENT     Sequence Label: pCB168
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             104..148
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtccgg aggggatcgg tggagctcca ccggtggcgg
       61 ccgctctaga gagaatcttt attttcaggg cgccgccaaa ttcaaagaaa ccgctgctgc
      121 taaattcgaa cgccagcaca tggacagcgg aggtggaggt actagtctcg agatgtctga
      181 caaaagtgac ttaaaagctg agctagagcg caaaaagcag cgcttagcac agataagaga
      241 agagaagaaa cggaaggaag aggagaggaa aaagaaagag gctgatatgc agcagaagaa
      301 agaacccgtt caggacgact ctgatctgga tcgcaaacga cgagagacag aggctttgct
      361 gcaaagcatt ggtatctcac cggagccgcc tctagtccca acccctatgt ctccctcctc
      421 gaaatcagtg agcactccca gtgaagctgg aagccaagac tcaggcgatc tggggccatt
      481 aacaaggacc ctgcagtggg acacagaccc ctcagtgctc cagctgcagt cagactcaga
      541 acttggaaga agactgcata aactgggcgt gtcaaaggtc acccaagtgg atttcctgcc
      601 aagggaagta gtgtcctact caaaggagac ccagactcct cttgccacgc atcagtctga
      661 agaggatgag gaagatgagg aaatggtgga atctaaagtt ggccaggact cagaactgga
      721 aaatcaggac aaaaaacagg aagtgaagga agcccctcca agagagttga cagaggaaga
      781 aaaacagcag atcattcatt cagaggaatt tctcatcttt tttgaccgga caatacgggt
      841 aattgaaaga gccctggctg aagattccga catctttttt gactacagcg gccgagagtt
      901 agaggaaaa
//
