LOCUS       sequence-48338-e         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Chromosome- and spindle-pole-derived signals generate an intrinsic 
            code for spindle position and orientation.
  JOURNAL   Nat Cell Biol. 2012 Feb 12;14(3):311-7. doi: 10.1038/ncb2440.
  PUBMED    22327364
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-02-12- 12"; volume: "14"; issue: "3"; 
            pages: "311-7"
COMMENT     Sequence Label: pCB169
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             102..146
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tcggcatgga cgagctgtac aagtccggag gggatcggtg gagctccacc ggtggcggcc
       61 gctctagaga gaatctttat tttcagggcg ccgccaaatt caaagaaacc gctgctgcta
      121 aattcgaacg ccagcacatg gacagcggag gtggaggtac tagtctcgag atggaggagt
      181 accatcgcca ctgcgacgag gttggcttca atgctgagga agcccacaat attgtcaaag
      241 agtgtgtaga tggggtttta ggtggtgaag attataatca caacaacatc aaccagtgga
      301 ctgcaagcat agtggaacaa tccttaacac acctggttaa gttgggaaaa gcctataaat
      361 atattgtgac ctgtgcagtg gtccagaaga gcgcatatgg ctttcacaca gccagctcct
      421 gtttttggga taccacatct gatggaacct gtaccgtaag atgggagaac cggaccatga
      481 actgtattgt caacgttttt gccattgcta ttgttcttta agaattctgc agtcgacggt
      541 accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca taccacattt
      601 gtagaggttt tacttgcttt aaaaaacctc ccaca
//
