LOCUS       sequence-48339-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Chromosome- and spindle-pole-derived signals generate an intrinsic 
            code for spindle position and orientation.
  JOURNAL   Nat Cell Biol. 2012 Feb 12;14(3):311-7. doi: 10.1038/ncb2440.
  PUBMED    22327364
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-02-12- 12"; volume: "14"; issue: "3"; 
            pages: "311-7"
COMMENT     Sequence Label: pTK5
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             297..317
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             330..374
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
       61 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
      121 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
      181 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
      241 agctgtacaa gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga
      301 atctttattt tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc
      361 agcacatgga cagcggaggt ggaggtacta gtctcgagat ggagtcctac gatgtgatcg
      421 ccaaccagcc tgtcgtgatc gacaacggat ccggtgtgat taaagctggt tttgctggtg
      481 atcagatccc caaatactgc tttccaaact atgtgggccg acccaagcac gttcgtgtca
      541 tggcaggagc ccttgaaggc gacatcttca ttggccccaa agctgaggag caccgagggc
      601 tgctttcaat ccgctatccc atggagcatg gcatcgtcaa ggattggaac gacatggaac
      661 gcatttggca atatgtctat tctaaggacc agctgcagac tttctcagag gagcatcctg
      721 tgctcctgac tgaggcgcct ttaaacccac gaaaaaaccg ggaacgagct gccgaagttt
      781 tcttcgagac cttcaatgtg cccgctcttt tcatctccat gcaagctgta ctcagccttt
      841 acgctacagg caggaccaca ggggtggtgc tggattctgg ggatggagtc acccatgctg
      901 tgcccatcta tgagggcttt gccatgccc
//
