LOCUS       sequence-483436-        5975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Archael (Heimdallarchaeota AB_125) 16S rRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Van Beek JM, Robles G, Mewalal R, Blaby I, Hatzenpichler RO
  TITLE     A collection of archaeal 16S rRNA Clone-FISH cultures for probe 
            validation in fluorescence in situ hybridization experiments
  JOURNAL   Microbiol Resour Announc. 2025 October. 0:e01131-25.
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc. 2025 October. 0:e01131-25."
COMMENT     Sequence Label: HeiAB125
FEATURES             Location/Qualifiers
     source          1..5975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        354..372
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1924..1971
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2009..2027)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2034..2051)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2034..2050)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2043..2065)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     oriT            2292..2401
                     /note="incP origin of transfer"
     rep_origin      2591..3019
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2678..2697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2888..2909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             3363..4157
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3417..3436)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4027..4046
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(4175..4243,4244..5035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4393..4412)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5206..5794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5695..5714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5948..5965
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagaa ttgtaatacg
      361 actcactata gggcgaatta agccggttga tcatgccgga cctaggtgct atcagattcg
      421 caatagacat gcaagtttaa tgtctcttcg gaggcatggc gaaaggctca gtaacacgtc
      481 gccaatctgc cctatggacg gggataacct cgggaaactg agattaatac ccgataggta
      541 tggaattctg gaaggagcca atacccaaag gggcttcggt tcgccatagg atgaggccgc
      601 ggccgattag ctagtaagtg atgtaaagga tcacttaggc gatgatcggt aggggtaatg
      661 agagttagcg ccccgagaag gacacttaga cactggtcct agcactacgg tgtgcagcag
      721 ttaggaattg tgcccaatgc gcgaaagcgt gagaccgcga atctgagtgc caaggagaac
      781 cttggctgtg atggagtgtt cgtagctcca acagcaagca gagggcaagg ctggtgccag
      841 ccgccgcggt aaaaccagct ctgcgagtgc ctaggacgat tattgggctt aaagcatccg
      901 tagccgggta agtaggtctc tgattaaatc tgcaagctta acttgtaggc tgtcagagat
      961 accgctaacc tagggaatag gagaggtgaa cggtactgcg agagaagcgg tgaaatgcgt
     1021 tgattctcgc aggacccaca gtggcgaagg cggttcactg gaatatctcc gacggtgatg
     1081 gatgaaagcc aggggagcga aagggattag agacccctgt agtcctggcc gtaaacgatg
     1141 aggattaggt gttggtcatg gctaagggcc tggtcagtgc caaagggaaa ctattaaatc
     1201 ctccgcctgg ggagtacggt cgcaaggctg aaacttaaat gaattgacgg gaaagcgcca
     1261 ccaggcacgg gatgtgtggt ttaattcgac tcaacgcgag gaaactcacc tggggcgact
     1321 gttacatgtg tgtcaggctg aagaccttac acgaataaaa cagagaggta gtgcatggcc
     1381 gtctcaagct cgtgccgtga ggtgtgctct taagtgagta aacgagcgag acccgcgccc
     1441 ctatttgcta agagcaagct tcggcttggc tgaggacaat agggggatcg ctatcgatga
     1501 agatagatga aagagcgggc cacggcaggt cagtatgctc caaatcccca gggctacaca
     1561 cgcatcacaa tggacaggac aatgagaagc gactccgaaa ggagaagcgg acctccaaac
     1621 ctgttcgcag tagggatcga ggtctggaac cgacctcgtg aacatggagc gcctagtatc
     1681 cgtgtgtcat catcgcacgg agaatacgtc cccgcttttt gtacacaccg cccgtcgttg
     1741 caacgaaatg aggttcggtt gaggctgagg cgaatcggtt cagtcaaaac tgggcctcgt
     1801 gacgatgcaa aagtcgtaac aaggtatccg taaaattggt gagaggcagt ctcccttttc
     1861 ggtaagggga acctgcggaa atacataagt cgaggcttat gtatcgcaag tggatcacct
     1921 cctctagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gtgcatgctc
     1981 gagcggccgc cagtgtgatg gaattcgccc tatagtgagt cgtattacaa ttcactggcc
     2041 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2101 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2161 caacagttgc gcagcctgaa tggcgaatgg acgcgctgga aggcagtaca ccttgatagg
     2221 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     2281 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     2341 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     2401 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     2461 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     2521 ttatgcagcg gaaaagcgct gcttccctgc tgttttgtgg aatatctacc gactggaaac
     2581 aggcaaatgc aggaaccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2641 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2701 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2761 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2821 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2881 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2941 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3001 caaaaattta acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga
     3061 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     3121 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     3181 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     3241 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     3301 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc
     3361 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3421 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3481 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3541 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3601 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3661 aggatctcct gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     3721 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     3781 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3841 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     3901 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3961 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4021 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4081 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4141 ttgacgagtt cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4201 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4261 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4321 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4381 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4441 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4501 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4561 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4621 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4681 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4741 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4801 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4861 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4921 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4981 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5041 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5101 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5161 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5221 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5281 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5341 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5401 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5461 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5521 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5581 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5641 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5701 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5761 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5821 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5881 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5941 cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
