LOCUS       sequence-483448-        5905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Archael (Thalassoarchaea mediterranii) 16S rRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Van Beek JM, Robles G, Mewalal R, Blaby I, Hatzenpichler RO
  TITLE     A collection of archaeal 16S rRNA Clone-FISH cultures for probe 
            validation in fluorescence in situ hybridization experiments
  JOURNAL   Microbiol Resour Announc. 2025 October. 0:e01131-25.
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc. 2025 October. 0:e01131-25."
COMMENT     Sequence Label: Thalmedi
FEATURES             Location/Qualifiers
     source          1..5905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        354..372
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1854..1901
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1939..1957)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1964..1981)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1964..1980)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1973..1995)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     oriT            2222..2331
                     /note="incP origin of transfer"
     rep_origin      2521..2949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2608..2627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2818..2839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             3293..4087
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3347..3366)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3957..3976
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(4105..4173,4174..4965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4323..4342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5136..5724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5625..5644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5878..5895
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttaagccga
      301 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagaa ttgtaatacg
      361 actcactata gggcgaatta aatccggttg atcctgccgg aggctaccgc tattggagtt
      421 cgattaagcc atgcgagtcc agagctttcg gagctcggcg taccgctcag taacacgtgg
      481 gtaacctaac ctatgcaggg gaataacctc gggaaactga gtataatgcc ccatagtcca
      541 ctacgcctgg aacggtgagt ggatcaaagc tcaggcggca taggatgggc ctgcggcgta
      601 tcaggttgta gggggtgtaa agtaccctct agccatctac gcgtacgggt gttgggagac
      661 atagcccgga gatggattct gagacacgaa tccagaccct acggggtgca gcaggcgcga
      721 aaacttgaca atgcgagcaa tcgtgatcag ggaactccaa gtggctgggg taagacccag
      781 tcttttcata actcttcaaa ggttgtagaa taaggggtgg gtaaggacgg tgccagccgc
      841 cgcggtaata ccggcgcctc aagtggtagt cgcttttatt gggcctaaaa cgtccgtagc
      901 cggtttggta cattcgtggg taaatcaact cgctcaacga gttgaattct gcgaggacgg
      961 ccagacttgg gaccgggaga ggtgtggggt actctcaggg taggggtaaa atcctgtcat
     1021 cctgagagga ccacctgttg cgaaggcgcc acactagaac ggatccgacg gtcagggacg
     1081 aagcctaggg gcacgaaccg gattagatac ccgggtagtc ctaggtgtaa acgctgtgga
     1141 cttgatgttg gagaagctcc gagcttcttc agtgtcgaag cgaaggtgat aagtccactg
     1201 cctgggaagt acggtcgcaa ggctgaaact taaaggaatt ggcgggggag caccgcaacc
     1261 tgaggattgt gcggtttaat tggattcaac gccggaaaac tcaccatagc cgacggacat
     1321 atgaaggtca gtgtaatgaa cttaccagat tgttcgagag gtagtgcatg gccgccgtca
     1381 gttcgtaccg taaggcgttc tgttaagtca gataacgaac gagaccctca tctctatttg
     1441 ccaccagatc tctggatctg acgggactat agagagaccg ctagcgcgaa gttagaggaa
     1501 ggggagggca acggtaggtc agtatgctcc gaatgctatg ggctacacgc gcaatacaaa
     1561 ggacgggaca atgggctgct actccgaaag gaatgacgct atcccgaaac ccgttcgtag
     1621 ttcggattga gggctgtaac tcgccctcat gaagctggat taggtagtaa tcgcgtctca
     1681 ataaggcgcg gtgaatatgc ccctgctcct tgcacacacc gcccgtcaaa ccatcttagt
     1741 tgtgggtggg tgaggctacc tcttattggg gtattcgagc ctgccttcga caaggagggt
     1801 taagtcgtaa caaggtatct gtaggggaac ctgcagatgg atcacctcct acgctagcat
     1861 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gtgcatgctc gagcggccgc
     1921 cagtgtgatg gaattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     1981 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2041 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2101 gcagcctgaa tggcgaatgg acgcgctgga aggcagtaca ccttgatagg tgggctgccc
     2161 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
     2221 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     2281 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     2341 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     2401 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     2461 gaaaagcgct gcttccctgc tgttttgtgg aatatctacc gactggaaac aggcaaatgc
     2521 aggaaccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2581 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2641 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2701 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     2761 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2821 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2881 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     2941 acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga acacgtagaa
     3001 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
     3061 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
     3121 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
     3181 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
     3241 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     3301 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3361 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     3421 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     3481 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     3541 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     3601 gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     3661 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     3721 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     3781 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     3841 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     3901 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     3961 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4021 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4081 cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4141 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4201 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4261 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4321 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4381 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4441 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4501 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4561 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4621 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4681 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4741 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4801 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4861 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4921 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4981 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5041 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5101 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5161 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5221 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5281 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5341 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5401 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5461 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5521 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5581 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5641 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5701 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5761 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5821 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5881 gagtcagtga gcgaggaagc ggaag
//
