LOCUS       sequence-483465-       11192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SunTag-Renilla mRNA reporter in mammalian cells; note 
            plasmid contains 24xGCN4 repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Wilbertz JH, Voigt F, Horvathova I, Roth G, Zhan Y, Chao JA
  TITLE     Single-Molecule Imaging of mRNA Localization and Regulation during 
            the Integrated Stress Response.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 
            10.1016/j.molcel.2018.12.006.
  PUBMED    30661979
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 
            10.1016/j.molcel.2018.12.006."
COMMENT     Sequence Label: pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop
            2...
FEATURES             Location/Qualifiers
     source          1..11192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1521..1990
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2055..2412
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     2823..2845
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2861..2877
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    2930..2963
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    3071..3205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3108..3127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4735..5058)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     CDS             complement(join(4738..5058,5059..5061))
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             complement(5062..5991)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             complement(6025..6081)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6169..6225)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6241..6297)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6313..6369)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6385..6441)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6457..6513)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6529..6585)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6601..6657)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6673..6729)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6745..6801)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6817..6873)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6889..6945)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6961..7017)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7033..7089)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7105..7161)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7177..7233)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7249..7305)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7321..7377)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7393..7449)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7465..7521)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7537..7593)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7609..7665)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7681..7737)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     regulatory      7803..7812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7816..7834)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7877..7896)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          7879..8011
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(8088..8112)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8157..8175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8193..8211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8229..8247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8265..8283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8301..8319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8337..8355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8373..8391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8438..8485
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(9567..9584)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9567..9583)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9576..9598)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9792..9811)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9911..9933
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9971..9989)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10057..10161
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10162..10230,10231..11022)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10380..10399)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctag ctttgctctt
     1021 aggagtttcc taatacatcc caaactcaaa tatataaagc atttgacttg ttctatgccc
     1081 tagggggcgg ggggaagcta agccagcttt ttttaacatt taaaatgtta attccatttt
     1141 aaatgcacag atgtttttat ttcataaggg tttcaatgtg catgaatgct gcaatattcc
     1201 tgttaccaaa gctagtataa ataaaaatag ataaacgtgg aaattactta gagtttctgt
     1261 cattaacgtt tccttcctca gttgacaaca taaatgcgct gctgagaagc cagtttgcat
     1321 ctgtcaggat caatttccca ttatgccagt catattaatt actagtcaat tagttgattt
     1381 ttatttttga catatacatg tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     1441 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag
     1501 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     1561 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     1621 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     1681 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     1741 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     1801 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
     1861 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     1921 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     1981 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
     2041 caccaccggg aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct
     2101 atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg
     2161 acccgtggtg gaactgacga gttcggaaca cccggccgca accctgggag acgtcccagg
     2221 gacttcgggg gccgtttttg tggcccgacc tgagtcctaa aatcccgatc gtttaggact
     2281 ctttggtgca ccccccttag aggagggata tgtggttctg gtaggagacg agaacctaaa
     2341 acagttcccg cctccgtctg aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg
     2401 tcttgtctgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     2461 tgaaaatatg ggcccgggct agcctgttac cactccctta agtttgacct taggtcactg
     2521 gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt
     2581 taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt
     2641 taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca
     2701 cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg
     2761 ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
     2821 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
     2881 ttctctaggc gcccccatat ggccatatga gatccgaagt tcctattccg aagttcctat
     2941 tcttcaaata gtataggaac ttcagatcat aatcagccat accacatttg tagaggtttt
     3001 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3061 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaggca atagcatcac
     3121 aaatttcaca aataaggcat ttttttcact gcattctagt tttggtttgt ccaaactcat
     3181 caatgtatct tatcatgtct ggatctcaaa tccctcggaa gctgcgcctg tcttaggttg
     3241 gagtgataca tttttatcac ttttacccgt ctttggatta ggcagtagct ctgacggccc
     3301 tcctgtctta ggttagtgaa aaatgtcact ctcttacccg tcattggctg tccagcttag
     3361 ctcgcagggg aggtggtctg gatcccgcgc gaaatgagat ctgaggtgtt tgatgtacgg
     3421 tgcggtgatc ctgcaccgtt tgcctggttg taagtttgtg ggttgtcggc ggatgccgtg
     3481 ttcgtgcatc cgtttgtcgt gtttttgtga ggttttccgc cgcgggaagg ctgttgctct
     3541 tcccgtcgga tgtttttcgt aatagtttgc gctggacgaa agcgtgatcg tctttcgtat
     3601 ttgtaaacca gttgattttt ccgtgtgtac cgttcgctgt tgctgaacgg tttggttttt
     3661 gatagtagtg agagatgtgg gctggtacgc tgatgctgta ccagtaattg tttttgattt
     3721 ggtatgttgg aatgggcaag cggtgatcct gcttgccagt tttgtgggaa gaggtttgag
     3781 gtaggagtgg gttcgctggt gctgaaccca tttggtagtt tctgtggttg agacttgtgg
     3841 agcatgccgt gatcgtgcat gcttgaatgt cctgagtgga agtgatgtag ctgcgggatc
     3901 cgtgatcgtg atcccgaaag gtaagtaatc gtattggacg tggaacgacg agccctggtg
     3961 gtgctcgtcc ttgtccgtgt tttgaaattc gtgagagcat gggtggcgtg atcgtccacc
     4021 caagaaatag gcacaatgta tgctcgagtg tttcgaagcg ctgatgctgc ttcgagaaga
     4081 gtagtttttg tcttgttggt gagagtccat ggcctgatgg tccatggagg gtttgtccag
     4141 ttggacaaga aacaacactc cgagccctgt tggtgctcgg atgtgatttt gttggggatg
     4201 tattcttggg gcccatgcgc tgatgctgca tggggacata aattttcttg agttgggtac
     4261 tgggcaggaa cgctgatgct gttcctgtag gggagagtgt ggtttgtgta agaatggcgc
     4321 gaaggctgat gctcttcgcg aaagtcgact tgtattcgac tctgattggc tgcgtgggct
     4381 gatgctccac gcactgaatt cgaaagccag acatgccgat attcgggagg cgtgatcgtc
     4441 ctcccgtcgt ggccctcgtt tgacgtatat tgcacaggga atccctgatg gtgattcctt
     4501 gttgtagttt tttgagataa atttggtagc agaagccctg atggtgcttc tgtttgattg
     4561 gatttctgtg taatgtgtct ggagggtggg gtgttcctcc acccttgtgt attgtacccc
     4621 ttgattgtga agtgtcgggt ggggtgatcc tccacccgtt tgtaggttgg atccgtttaa
     4681 acttgaagct acaacatgtg gggtcctctt tccgagccag ttcttccctg gcccttattc
     4741 cggttttaga agctccacat cgaagacgag agtggcatgt ggtgggatga tgcctgggtg
     4801 cccagtggca ccataggcat aatctggaga tatagtcagt ttggctctct gacccacact
     4861 catctgggca accccttctt cccagcctcg gatcacctcc tgcttgccta gcataaactt
     4921 aaagggcttg tttctgtccc gggaggaatc gactttcttt ccatcttcaa gcatcccggt
     4981 gtagtgcacc acacaggtct ggccgcgctt ggggaaggtg cgcccgtctc ctggggagat
     5041 ggtttccacc tgcactccca tctgctcgtt cttcagcacg cgctccacga agctcttgat
     5101 gtacttaccc atttcatctg gagcgtcctc ctggctgaag tggaggccct tcaccttcac
     5161 gaactcggtg ttagggaact tcttagctcc ctcgacaata gcgttggaaa agaacccagg
     5221 gtcggactcg atgaacatct taggcagatc gtcgctggcc cgaaggtagg cgttgtagtt
     5281 gcggacaatc tggacgacgt cgggcttgcc tcccttaacg agagggatct cgcgaggcca
     5341 ggagagggta ggccgtctaa cctcgccctt ctccttgaat ggctccaggt aggcagcgaa
     5401 ctcctcaggc tccagtttcc gcatgatctt gcttgggagc atggtctcga cgaagaagtt
     5461 attctcaagc accattttct cgccctcttc gctcttgatc agggcgatat cctcctcgat
     5521 gtcaggccac tcgtcccagg actcgatcac gtccacgaca ctctcagcat ggacgatggc
     5581 cttgatcttg tcttggtgct cgtaggagta gtgaaaggcc agacaagccc cccagtcgtg
     5641 gcccacaaag atgattttct ttggaaggtt cagcagctcg aaccaagcgg tgaggtactt
     5701 gtagtgatcc aggaggcgat atgagccatt cccgctcttg ccggacttac ccattccgat
     5761 cagatcaggg atgatgcatc tagccacggg ctcgatgtga ggcacgacgt gcctccacag
     5821 gtagctggag gcagcgttac catgcagaaa aatcacggcg ttctcggcgt gcttctcgga
     5881 atcatagtag ttgatgaagg agtccagcac gttcatttgc ttgcagcgag cccaccactg
     5941 aggcccagtg atcatgcgtt tgcgttgctc ggggtcgtac accttggaag ctgaccggtg
     6001 cggccgctga cccgagccag aacccttttt aagtcgggct acttcattct cgagatgata
     6061 atttttcgac agaagttcct caccactgcc acttcccttt tttaaacgtg caacctcatt
     6121 ttccaaatga taatcttttg agagcagttc ttcacccgac cctgaacctt tctttaatcg
     6181 agctacttcg ttttccaggt ggtaattctt actgagtagt tcctcacctg atccggaccc
     6241 ctttttaagt cttgcaacct cattttcaag atgatagttc ttcgacagga gttcctcccc
     6301 actgccggag cccttcttta gtcgagccac ctcgttctcg agatgataat tcttagatag
     6361 taactcttcc ccgctcccgc tccctttctt caagcgcgcc acctcatttt ccaagtggta
     6421 gttcttgctc aagagctctt ccccgctccc cgagcctttc ttcaagcggg cgacttcatt
     6481 ctctaagtgg tagttcttcg ataagagctc ctccccgctg ccgctccctt tcttgagcct
     6541 tgccacttcg ttctccaagt ggtaattttt gctcaataac tcttcgccgc tcccactccc
     6601 ttttttgagc ctggcgactt cattttccaa gtggtagttt ttactcaaca attcctcccc
     6661 cgaccccgag cctttcttga gcctggcgac ttcgttctcc aaatgataat tcttgctgag
     6721 caattcctcg ccgctccccg accctttctt caatctcgcg acctcattct ccaagtggta
     6781 gttcttgctc aagagttcct ccccgctgcc actgcccttt ttcaacctgg cgacctcatt
     6841 ttcgaggtgg tagttcttcg ataagagttc ttcgcccgag cccgagcctt tctttaagcg
     6901 cgcgacttcg ttctctaaat gatagttttt gctcaataac tcctcgcccg acccgctccc
     6961 ctttttcaac ctcgcgacct cattttccaa gtggtagttt ttcgagagca actcctcgcc
     7021 tgagcctgag ccctttttga gcctagcaac ttcgttctca agatgataat ttttcgatag
     7081 caactcttcg ccagaaccac tccccttttt tagtctcgct acctcatttt ccagatgata
     7141 atttttactc agtaattctt cacccgaccc tgaacctttc tttaatcgag ctacttcgtt
     7201 ttcgaggtgg taattctttg aaagtagttc ctcaccactg cccgatccct tttttaatcg
     7261 agcgacctca ttttcaagat gatagttctt cgagagcagt tcctctcctg agccggaacc
     7321 cttcttcaaa cgcgctacct cgttctcaag atgataattc ttggatagta gctcttcacc
     7381 actgccactt ccctttttta agcgtgccac ctcattttcg aggtggtagt ttttagataa
     7441 tagctcttct ccagaaccac tgcccttttt tagccgagcc acttcgtttt cgagatgata
     7501 atttttcgag agtaactcct cacccgatcc ggaccctttc tttagtcgtg ctacttcgtt
     7561 ctccaaatga taatttttgc tcagcaactc ctccccactt ccgctcccct ttttcagtct
     7621 agctacctca ttttccaggt ggtaattctt tgaaagcagt tcttctccac tgccagaacc
     7681 tttcttaaga cgagccactt cgttctcaag atgataattc ttgctcaaaa gttcttctcc
     7741 tccagaacct ccacctccag atccacccgg accgatgatt gcggccgcgt gtatgatggc
     7801 gcccatggtg gctagcctat agtgagtcgt attaagtact ctagccttaa gagctgtaat
     7861 tgaactggga gtggacacct gtggagagaa aggcaaagtg gatgtcagta agaccaatag
     7921 gtgcctatca gaaacgcaag agtcttctct gtctcgacaa gcccagtttc tattggtctc
     7981 cttaaacctg tcttgtaacc ttgatactta cctgaccggt tttacgaggg taggaagtgg
     8041 tacggaaagt tggtataaga caaaagtgtt gtggaattgc tccaggcgat ctgacggttc
     8101 actaaacgag ctctgctttt ataggcgccc accgtacacg cctaaagctt atacgttctc
     8161 tatcactgat agggagtaaa ctggatatac gttctctatc actgataggg agtaaactgt
     8221 agatacgttc tctatcactg atagggagta aactggtcat acgttctcta tcactgatag
     8281 ggagtaaact ccttatacgt tctctatcac tgatagggag taaagtctgc atacgttctc
     8341 tatcactgat agggagtaaa ctcttcatac gttctctatc actgataggg agtaaagaat
     8401 tcggccggcc caaagcggcc gcaaaagatc tggccgcgaa gttcctattc cgaagttcct
     8461 attctctaga aagtatagga acttcagatc cggattagtc caatttgtta aagacaggat
     8521 atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta
     8581 cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     8641 ccccacctgt aggtttggca agctagctcg acttctgttc gcgcgcttct gctccccgag
     8701 ctcgagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     8761 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     8821 gagagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
     8881 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagtacttaa
     8941 agttacattg gcttccttga aataaacatg gagtattcag aatgtgtcat aaatatttct
     9001 aattttaaga tagtatctcc attggctttc tactttttct tttatttttt tttgtcctct
     9061 gtcttccatt tgttgttgtt gttgtttgtt tgtttgtttg ttggttggtt ggttaatttt
     9121 tttttaaaga tcctacacta tagttcaagc tagactatta gctactctgt aacccagggt
     9181 gaccttgaag tcatgggtag cctgctgttt tagccttccc acatctaaga ttacaggtat
     9241 gagctatcat ttttggtata ttgattgatt gattgattga tgtgtgtgtg tgtgattgtg
     9301 tttgtgtgtg tgattgtgta tatgtgtgta tggttgtgtg tgattgtgtg tatgtatgtt
     9361 tgtgtgtgat tgtgtgtgtg tgattgtgca tgtgtgtgtg tgtgattgtg tttatgtgta
     9421 tgattgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtt gtgtatatat
     9481 atttatggta gtgagaggca acgctccggc tcaggtgtca ggttggtttt tgagacagag
     9541 tctttcactt agcttggaat taattcactg gccgtcgttt tacaacgtcg tgactgggaa
     9601 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     9661 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     9721 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     9781 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     9841 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     9901 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     9961 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
    10021 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10081 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    10141 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    10201 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    10261 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    10321 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    10381 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    10441 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    10501 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10561 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10621 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10681 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10741 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10801 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10861 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10921 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10981 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    11041 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    11101 atcctttttg ataatctcat gacaaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11161 tcagaccccg tagaaaagat caaaggatct tc
//
