LOCUS       sequence-483544-       13789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MMLVgag–DNMT3A-3L-dCas9-ZIM3 for producing 
            RENDER-DNMT3A-3L-dCas9-ZIM3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Xu D, Besselink S, Ramadoss GN, Dierks PH, Lubin JP, Pattali RK, 
            Brim JI, Christenson AE, Colias PJ, Ornelas IJ, Nguyen CD, Chasins 
            SE, Conklin BR, Nunez JK
  TITLE     Programmable epigenome editing by transient delivery of CRISPR 
            epigenome editor ribonucleoproteins.
  JOURNAL   Nat Commun. 2025 Aug 26;16(1):7948. doi: 10.1038/s41467-025-63167-x.
  PUBMED    40858609
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-08-26"; volume: "16(1):7948. doi"; pages: " 
            10.1038/s41467-025-63167-x"
COMMENT     Sequence Label: pCMV-MMLVgag-D3A-3L-dCas9-ZIM3
FEATURES             Location/Qualifiers
     source          1..13789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1785..1804
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1924..2303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2304..2507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2458..2478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2504..2528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2619..3191
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             3289..3705
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3635..3657
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3673..3689
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4918..4944
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4957..4983
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4996..5022
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5056..5079
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5113..5133
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7003..7029,7030..7032,7033..9519,9520..9522,
                     9523..11106)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11110..11136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             11116..11142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             11155..11175
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11182..11202
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11242..11937
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             12295..12315
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(12321..12340)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    12386..12441
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(12440..12459)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(12799..12815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12799..12815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12812..12834)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12823..12839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12847..12853,12854..12871,12872..12877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12892..12913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13030..13047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13201..13789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13281..13300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt ggagctccac cgcggtggcg gccgctctag agagcttggc ccattgcata
     1861 cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca ttaccgccat
     1921 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     1981 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2041 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2101 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2161 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2221 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2281 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2341 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2401 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2461 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2521 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     2581 gatccagcct ccggtcgacc gatcctgaga acttcagggt gagtttgggg acccttgatt
     2641 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
     2701 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     2761 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     2821 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
     2881 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
     2941 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
     3001 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
     3061 ccctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
     3121 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
     3181 ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag
     3241 aattcctcga gggccggatc cgaattcctg tatttgtctg aaaacatggg ccagactgtt
     3301 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
     3361 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
     3421 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
     3481 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
     3541 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
     3601 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
     3661 cctcgatcct ccctttatcc agccctcact ccttctctag gcgccaaacc taaacctcaa
     3721 gttctttctg acagtggggg gccgctcatc gacctactta cagaagaccc cccgccttat
     3781 agggacccaa gaccaccccc ttccgacagg gacggaaatg gtggagaagc gacccctgcg
     3841 ggagaggcac cggacccctc cccaatggca tctcgcctac gtgggagacg ggagccccct
     3901 gtggccgact ccactacctc gcaggcattc cccctccgcg caggaggaaa cggacagctt
     3961 caatactggc cgttctcctc ttctgacctt tacaactgga aaaataataa cccttctttt
     4021 tctgaagatc caggtaaact gacagctctg atcgagtctg tcctcatcac ccatcagccc
     4081 acctgggacg actgtcagca gctgttgggg actctgctga ccggagaaga aaaacaacgg
     4141 gtgctcttag aggctagaaa ggcggtgcgg ggcgatgatg ggcgccccac tcaactgccc
     4201 aatgaagtcg atgccgcttt tcccctcgag cgcccagact gggattacac cacccaggca
     4261 ggtaggaacc acctagtcca ctatcgccag ttgctcctag cgggtctcca aaacgcgggc
     4321 agaagcccca ccaatttggc caaggtaaaa ggaataacac aagggcccaa tgagtctccc
     4381 tcggccttcc tagagagact taaggaagcc tatcgcaggt acactcctta tgaccctgag
     4441 gacccagggc aagaaactaa tgtgtctatg tctttcattt ggcagtctgc cccagacatt
     4501 gggagaaagt tagagaggtt agaagattta aaaaacaaga cgcttggaga tttggttaga
     4561 gaggcagaaa agatctttaa taaacgagaa accccggaag aaagagagga acgtatcagg
     4621 agagaaacag aggaaaaaga agaacgccgt aggacagagg atgagcagaa agagaaagaa
     4681 agagatcgta ggagacatag agagatgagc aagctattgg ccactgtcgt tagtggacag
     4741 aaacaggata gacagggagg agaacgaagg aggtcccaac tcgatcgcga ccagtgtgcc
     4801 tactgcaaag aaaaggggca ctgggctaaa gattgtccca agaaaccacg aggacctcgg
     4861 ggaccaagac cccagacctc cctcctgacc ctagatgact ctggcggctc acttcaactg
     4921 cctccacttg aaagactgac actgggatca ttacaattac ctcctttaga acgattaaca
     4981 ctcggttcac tacagcttcc gcctcttgag agattgacat taacgtccac gctgctaatg
     5041 gagaactcgt ctggagatta caaagacgat gacgataaga aacggacagc cgacggaagc
     5101 gagttcgagt caccaaagaa gaagcggaaa gtcaaccatg accaggaatt tgacccccca
     5161 aaggtttacc cacctgtgcc agctgagaag aggaagccca tccgcgtgct gtctctcttt
     5221 gatgggattg ctacagggct cctggtgctg aaggacctgg gcatccaagt ggaccgctac
     5281 attgcctccg aggtgtgtga ggactccatc acggtgggca tggtgcggca ccagggaaag
     5341 atcatgtacg tcggggacgt ccgcagcgtc acacagaagc atatccagga gtggggccca
     5401 ttcgacctgg tgattggagg cagtccctgc aatgacctct ccattgtcaa ccctgcccgc
     5461 aagggacttt atgagggtac tggccgcctc ttctttgagt tctaccgcct cctgcatgat
     5521 gcgcggccca aggagggaga tgatcgcccc ttcttctggc tctttgagaa tgtggtggcc
     5581 atgggcgtta gtgacaagag ggacatctcg cgatttcttg agtctaaccc cgtgatgatt
     5641 gacgccaaag aagtgtctgc tgcacacagg gcccgttact tctggggtaa ccttcctggc
     5701 atgaacaggc ctttggcatc cactgtgaat gataagctgg agctgcaaga gtgtctggag
     5761 cacggcagaa tagccaagtt cagcaaagtg aggaccatta ccaccaggtc aaactctata
     5821 aagcagggca aagaccagca tttccccgtc ttcatgaacg agaaggagga catcctgtgg
     5881 tgcactgaaa tggaaagggt gtttggcttc cccgtccact acacagacgt ctccaacatg
     5941 agccgcttgg cgaggcagag actgctgggc cgatcgtgga gcgtgccggt catccgccac
     6001 ctcttcgctc cgctgaagga atattttgct tgtgtgtcta gcggcaatag taacgctaac
     6061 agccgcgggc cgagcttcag cagcggcctg gtgccgttaa gcttgcgcgg cagccatatg
     6121 ggccctatgg agatatacaa gacagtgtct gcatggaaga gacagccagt gcgggtactg
     6181 agcctcttca gaaacatcga caaggtacta aagagtttgg gcttcttgga aagcggttct
     6241 ggttctgggg gaggaacgct gaagtacgtg gaagatgtca caaatgtcgt gaggagagac
     6301 gtggagaaat ggggcccctt tgacctggtg tacggctcga cgcagcccct aggcagctct
     6361 tgtgatcgct gtcccggctg gtacatgttc cagttccacc ggatcctgca gtatgcgctg
     6421 cctcgccagg agagtcagcg gcccttcttc tggatattca tggacaatct gctgctgact
     6481 gaggatgacc aagagacaac tacccgcttc cttcagacag aggctgtgac cctccaggat
     6541 gtccgtggca gagactacca gaatgctatg cgggtgtgga gcaacattcc agggctgaag
     6601 agcaagcatg cgcccctgac cccaaaggaa gaagagtatc tgcaagccca agtcagaagc
     6661 aggagcaagc tggacgcccc gaaagttgac ctcctggtga agaactgcct tctcccgctg
     6721 agagagtact tcaagtattt ttctcaaaac tcacttcctc ttggagggcc gagctctggc
     6781 gcacccccac caagtggagg gtctcctgcc gggtccccaa catctactga agaaggcacc
     6841 agcgaatccg caacgcccga gtcaggccct ggtacctcca cagaaccatc tgaaggtagt
     6901 gcgcctggtt ccccagctgg aagccctact tccaccgaag aaggcacgtc aaccgaacca
     6961 agtgaaggat ctgcccctgg gaccagcact gaaccatctg agatggacaa gaagtattct
     7021 atcggactgg ccatcgggac taatagcgtc gggtgggccg tgatcactga cgagtacaag
     7081 gtgccctcta agaagttcaa ggtgctcggg aacaccgacc ggcattccat caagaaaaat
     7141 ctgatcggag ctctcctctt tgattcaggg gagaccgctg aagcaacccg cctcaagcgg
     7201 actgctagac ggcggtacac caggaggaag aaccggattt gttaccttca agagatattc
     7261 tccaacgaaa tggcaaaggt cgacgacagc ttcttccata ggctggaaga atcattcctc
     7321 gtggaagagg ataagaagca tgaacggcat cccatcttcg gtaatatcgt cgacgaggtg
     7381 gcctatcacg agaaataccc aaccatctac catcttcgca aaaagctggt ggactcaacc
     7441 gacaaggcag acctccggct tatctacctg gccctggccc acatgatcaa gttcagaggc
     7501 cacttcctga tcgagggcga cctcaatcct gacaatagcg atgtggataa actgttcatc
     7561 cagctggtgc agacttacaa ccagctcttt gaagagaacc ccatcaatgc aagcggagtc
     7621 gatgccaagg ccattctgtc agcccggctg tcaaagagcc gcagacttga gaatcttatc
     7681 gctcagctgc cgggtgaaaa gaaaaatgga ctgttcggga acctgattgc tctttcactt
     7741 gggctgactc ccaatttcaa gtctaatttc gacctggcag aggatgccaa gctgcaactg
     7801 tccaaggaca cctatgatga cgatctcgac aacctcctgg cccagatcgg tgaccaatac
     7861 gccgaccttt tccttgctgc taagaatctt tctgacgcca tcctgctgtc tgacattctc
     7921 cgcgtgaaca ctgaaatcac caaggcccct ctttcagctt caatgattaa gcggtatgat
     7981 gagcaccacc aggacctgac cctgcttaag gcactcgtcc ggcagcagct tccggagaag
     8041 tacaaggaaa tcttctttga ccagtcaaag aatggatacg ccggctacat cgacggaggt
     8101 gcctcccaag aggaatttta taagtttatc aaacctatcc ttgagaagat ggacggcacc
     8161 gaagagctcc tcgtgaaact gaatcgggag gatctgctgc ggaagcagcg cactttcgac
     8221 aatgggagca ttccccacca gatccatctt ggggagcttc acgccatcct tcggcgccaa
     8281 gaggacttct acccctttct taaggacaac agggagaaga ttgagaaaat tctcactttc
     8341 cgcatcccct actacgtggg acccctcgcc agaggaaata gccggtttgc ttggatgacc
     8401 agaaagtcag aagaaactat cactccctgg aacttcgaag aggtggtgga caagggagcc
     8461 agcgctcagt cattcatcga acggatgact aacttcgata agaacctccc caatgagaag
     8521 gtcctgccga aacattccct gctctacgag tactttaccg tgtacaacga gctgaccaag
     8581 gtgaaatatg tcaccgaagg gatgaggaag cccgcattcc tgtcaggcga acaaaagaag
     8641 gcaattgtgg accttctgtt caagaccaat agaaaggtga ccgtgaagca gctgaaggag
     8701 gactatttca agaaaattga atgcttcgac tctgtggaga ttagcggggt cgaagatcgg
     8761 ttcaacgcaa gcctgggtac ctaccatgat ctgcttaaga tcatcaagga caaggatttt
     8821 ctggacaatg aggagaacga ggacatcctt gaggacattg tcctgactct cactctgttc
     8881 gaggaccggg aaatgatcga ggagaggctt aagacctacg cccatctgtt cgacgataaa
     8941 gtgatgaagc aacttaaacg gagaagatat accggatggg gacgccttag ccgcaaactc
     9001 atcaacggaa tccgggacaa acagagcgga aagaccattc ttgatttcct taagagcgac
     9061 ggattcgcta atcgcaactt catgcaactt atccatgatg attccctgac ctttaaggag
     9121 gacatccaga aggcccaagt gtctggacaa ggtgactcac tgcacgagca tatcgcaaat
     9181 ctggctggtt cacccgctat taagaagggt attctccaga ccgtgaaagt cgtggacgag
     9241 ctggtcaagg tgatgggtcg ccataaacca gagaacattg tcatcgagat ggccagggaa
     9301 aaccagacta cccagaaggg acagaagaac agcagggagc ggatgaaaag aattgaggaa
     9361 gggattaagg agctcgggtc acagatcctt aaagagcacc cggtggaaaa cacccagctt
     9421 cagaatgaga agctctatct gtactacctt caaaatggac gcgatatgta tgtggaccaa
     9481 gagcttgata tcaacaggct ctcagactac gacgtggacg ccatcgtccc tcagagcttc
     9541 ctcaaagacg actcaattga caataaggtg ctgactcgct cagacaagaa ccggggaaag
     9601 tcagataacg tgccctcaga ggaagtcgtg aaaaagatga agaactattg gcgccagctt
     9661 ctgaacgcaa agctgatcac tcagcggaag ttcgacaatc tcactaaggc tgagaggggc
     9721 ggactgagcg aactggacaa agcaggattc attaaacggc aacttgtgga gactcggcag
     9781 attactaaac atgtcgccca aatccttgac tcacgcatga ataccaagta cgacgaaaac
     9841 gacaaactta tccgcgaggt gaaggtgatt accctgaagt ccaagctggt cagcgatttc
     9901 agaaaggact ttcaattcta caaagtgcgg gagatcaata actatcatca tgctcatgac
     9961 gcatatctga atgccgtggt gggaaccgcc ctgatcaaga agtacccaaa gctggaaagc
    10021 gagttcgtgt acggagacta caaggtctac gacgtgcgca agatgattgc caaatctgag
    10081 caggagatcg gaaaggccac cgcaaagtac ttcttctaca gcaacatcat gaatttcttc
    10141 aagaccgaaa tcacccttgc aaacggtgag atccggaaga ggccgctcat cgagactaat
    10201 ggggagactg gcgaaatcgt gtgggacaag ggcagagatt tcgctaccgt gcgcaaagtg
    10261 ctttctatgc ctcaagtgaa catcgtgaag aaaaccgagg tgcaaaccgg aggcttttct
    10321 aaggaatcaa tcctccccaa gcgcaactcc gacaagctca ttgcaaggaa gaaggattgg
    10381 gaccctaaga agtacggcgg attcgattca ccaactgtgg cttattctgt cctggtcgtg
    10441 gctaaggtgg aaaaaggaaa gtctaagaag ctcaagagcg tgaaggaact gctgggtatc
    10501 accattatgg agcgcagctc cttcgagaag aacccaattg actttctcga agccaaaggt
    10561 tacaaggaag tcaagaagga ccttatcatc aagctcccaa agtatagcct gttcgaactg
    10621 gagaatgggc ggaagcggat gctcgcctcc gctggcgaac ttcagaaggg taatgagctg
    10681 gctctcccct ccaagtacgt gaatttcctc taccttgcaa gccattacga gaagctgaag
    10741 gggagccccg aggacaacga gcaaaagcaa ctgtttgtgg agcagcataa gcattatctg
    10801 gacgagatca ttgagcagat ttccgagttt tctaaacgcg tcattctcgc tgatgccaac
    10861 ctcgataaag tccttagcgc atacaataag cacagagaca aaccaattcg ggagcaggct
    10921 gagaatatca tccacctgtt caccctcacc aatcttggtg cccctgccgc attcaagtac
    10981 ttcgacacca ccatcgaccg gaaacgctat acctccacca aagaagtgct ggacgccacc
    11041 ctcatccacc agagcatcac cggactttac gaaactcgga ttgacctctc acagctcgga
    11101 ggggatgcct atccctatga cgtgcccgat tatgccagcc tgggcagcgg ctcccccaag
    11161 aaaaaacgca aggtggaaga tcctaagaaa aagcggaaag tggacggcat tggtagtggg
    11221 agcaacggca gcagcggatc cagcgagctg attaaggaga acatgcacat gaagctgtac
    11281 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
    11341 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
    11401 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
    11461 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
    11521 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
    11581 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
    11641 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
    11701 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc
    11761 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
    11821 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
    11881 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatggc
    11941 ggtggcggag ggatgaacaa ttcccaggga agagtgacct tcgaggatgt cactgtgaac
    12001 ttcacccagg gggagtggca gcggctgaat cccgaacaga gaaacttgta cagggatgtg
    12061 atgctggaga attacagcaa ccttgtctct gtgggacaag gggaaaccac caaacccgat
    12121 gtgatcttga ggttggaaca aggaaaggag ccatggttgg aggaagagga agtgctggga
    12181 agtggccgtg cagaaaaaaa tggggacatt ggagggcaga tttggaagcc aaaggatgtg
    12241 aaagagagtc tctctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag
    12301 aagaagcgga aagtctgaga tctttttccc tctgccaaaa attatgggga catcatgaag
    12361 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
    12421 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
    12481 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
    12541 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
    12601 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
    12661 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
    12721 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacggatcg
    12781 gccgcaattc gtaaccatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    12841 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    12901 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    12961 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    13021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    13081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    13141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    13201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    13261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    13321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    13381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtggg tcgttcgctc
    13441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    13501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    13561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    13621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    13681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    13741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa
//
