LOCUS       sequence-483593-        9815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Eco1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by Pm promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010-Eco1
FEATURES             Location/Qualifiers
     source          1..9815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            961..1069
                     /note="incP origin of transfer"
     CDS             complement(1080..1931)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1918..2757)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3268..4239)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      4346..4740
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(4778..4864)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4889..5854)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        6754..6834
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             6860..6882
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             8114..8122
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      9568..9662
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9783..9802)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(9794..9815,1..794)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 cgagctgtag ccgtccgcgt ctgaacagca acatggatgc ggatctgtat ggctataaat
       61 gggcgcgtga taacgtgggt cagagcggcg cgaccattta tcgtctgtat ggcaaaccgg
      121 atgcgccgga actgtttctg aaacatggca aaggcagcgt ggcgaacgat gtgaccgatg
      181 aaatggtgcg tctgaactgg ctgaccgaat ttatgccgct gccgaccatt aaacatttta
      241 ttcgcacccc ggatgatgcg tggctgctga ccaccgcgat tccgggcaaa accgcgtttc
      301 aggtgctgga agaatatccg gatagcggcg aaaacattgt ggatgcgctg gccgtgtttc
      361 tgcgtcgtct gcatagcatt ccggtgtgca actgcccgtt taacagcgat cgtgtgtttc
      421 gtctggccca ggcgcagagc cgtatgaaca acggcctggt ggatgcgagc gattttgatg
      481 atgaacgtaa cggctggccg gtggaacagg tgtggaaaga aatgcataaa ctgctgccgt
      541 ttagcccgga tagcgtggtg acccacggcg attttagcct ggataacctg attttcgatg
      601 aaggcaaact gattggctgc attgatgtgg gccgtgtggg cattgcggat cgttatcagg
      661 atctggccat tctgtggaac tgcctgggcg aatttagccc gagcctgcaa aaacgtctgt
      721 ttcagaaata tggcattgat aatccggata tgaacaaact gcaatttcat ctgatgctgg
      781 atgaattttt ctaataatta attggaccgc ggtccgcgcg ttgtcctttt ccgctgcata
      841 accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata
      901 tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc
      961 gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta
     1021 ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgt aggccggcct
     1081 cagcctgccg ccttgggccg ggtgatgtcg tacttgcccg ccgcgaactc ggttaccgtc
     1141 cagcccagcg cgaccagctc cggcaacgcc tcgcgcaccc gctggcggcg cttgcgcatg
     1201 gtcgaaccac tggcctctga cggccagaca tagccgcaca aggtatctat ggaagccttg
     1261 ccggttttgc cggggtcgat ccagccacac agccgctggt gcagcaggcg ggcggtttcg
     1321 ctgtccagcg cccgcacctc gtccatgctg atgcgcacat gctggccgcc acccatgacg
     1381 gcctgcgcga tcaaggggtt cagggccacg tacaggcgcc cgtccgcctc gtcgctggcg
     1441 tactccgaca gcagccgaaa cccctgccgc ttgcggccat tctgggcgat gatggatacc
     1501 ttccaaaggc gctcgatgca gtcctgtatg tgcttgagcg ccccaccact atcgacctct
     1561 gccccgattt cctttgccag cgcccgatag ctacctttga ccaccatggc atcagcggtg
     1621 acggcctccc acttgggttc caggaacagc cggagctgcc gtccgccttc ggtcttgggt
     1681 tccgggccaa gcactaggcc attaggccca gccatggcca ccagcccttg caggatgcgc
     1741 agatcatcag cgcccagcgg ctccgggccg ctgaactcga tccgcttgcc gtcgccgtag
     1801 tcatacgtca cgtccagctt gctgcgcttg cgctcgcccc gcttgagggc acggaacagg
     1861 ccgggggcca gacagtgcgc cgggtcgtgc cggacgtggc tgaggctgtg cttgttctta
     1921 ggcttcacca cggggcaccc ccttgctctt gcgctgcctc tccagcacgg cgggcttgag
     1981 caccccgccg tcatgccgcc tgaaccaccg atcagcgaac ggtgcgccat agttggcctt
     2041 gctcacaccg aagcggacga agaaccggcg ctggtcgtcg tccacacccc attcctcggc
     2101 ctcggcgctg gtcatgctcg acaggtagga ctgccagcgg atgttatcga ccagtaccga
     2161 gctgccccgg ctggcctgct gctggtcgcc tgcgcccatc atggccgcgc ccttgctggc
     2221 atggtgcagg aacacgatag agcacccggt atcggcggcg atggcctcca tgcgaccgat
     2281 gacctgggcc atggggccgc tggcgttttc ttcctcgatg tggaaccggc gcagcgtgtc
     2341 cagcaccatc aggcggcggc cctcggcggc gcgcttgagg ccgtcgaacc actccggggc
     2401 catgatgttg ggcaggctgc cgatcagcgg ctggatcagc aggccgtcag ccacggcttg
     2461 ccgttcctcg gcgctgaggt gcgccccaag ggcgtgcagg cggtgatgaa tggcggtggg
     2521 cgggtcttcg gcgggcaggt agatcaccgg gccggtgggc agttcgccca cctccagcag
     2581 atccggcccg cctgcaatct gtgcggccag ttgcagggcc agcatggatt taccggcacc
     2641 accgggcgac accagcgccc cgaccgtacc ggccaccatg ttgggcaaaa cgtagtccag
     2701 cggtggcggc gctgctgcga acgcctccag aatattgata ggcttatggg tagccattga
     2761 ttgcctcctt tgcaggcagt tggtggttag gcgctggcgg ggtcactacc cccgccctgc
     2821 gccgctctga gttcttccag gcactcgcgc agcgcctcgt attcgtcgtc ggtcagccag
     2881 aacttgcgct gacgcatccc tttggccttc atgcgctcgg catatcgcgc ttggcgtaca
     2941 gcgtcagggc tggccagcag gtcgccggtc tgcttgtcct tttggtcttt catatcagtc
     3001 accgagaaac ttgccggggc cgaaaggctt gtcttcgcgg aacaaggaca aggtgcagcc
     3061 gtcaaggtta aggctggcca tatcagcgac tgaaaagcgg ccagcctcgg ccttgtttga
     3121 cgtataacca aagccaccgg gcaaccaata gcccttgtca cttttgatca ggtagaccga
     3181 ccctgaagcg cttttttcgt attccataaa acccccttct gtgcgtgagt actcatagta
     3241 taacaggcgt gagtaccaac gcaagcacta catgctgaaa tctggcccgc ccctgtccat
     3301 gcctcgctgg cggggtgccg gtgcccgtgc cagctcggcc cgcgcaagct ggacgctggg
     3361 cagacccatg accttgctga cggtgcgctc gatgtaatcc gcttcgtggc cgggcttgcg
     3421 ctctgccagc gctgggctgg cctcggccat ggccttgccg atttcctcgg cactgcggcc
     3481 ccggctggcc agcttctgcg cggcgataaa gtcgcacttg ctgaggtcat caccgaagcg
     3541 cttgaccagc ccggccatct cgctgcggta ctcgtccagc gccgtgcgcc ggtggcggct
     3601 aagctgccgc tcgggcagtt cgaggctggc cagcctgcgg gccttctcct gctgccgctg
     3661 ggcctgctcg atctgctggc cagcctgctg caccagcgcc gggccagcgg tggcggtctt
     3721 gcccttggat tcacgcagca gcacccacgg ctgataaccg gcgcgggtgg tgtgcttgtc
     3781 cttgcggttg gtgaagcccg ccaagcggcc atagtggcgg ctgtcggcgc tggccgggtc
     3841 ggcgtcgtac tcgctggcca gcgtccgggc aatctgcccc cgaagttcac cgcctgcggc
     3901 gtcggccacc ttgacccatg cctgatagtt cttcgggctg gtttccacta ccagggcagg
     3961 ctcccggccc tcggctttca tgtcatccag gtcaaactcg ctgaggtcgt ccaccagcac
     4021 cagaccatgc cgctcctgct cggcgggcct gatatacacg tcattgccct gggcattcat
     4081 ccgcttgagc catggcgtgt tctggagcac ttcggcggct gaccattccc ggttcatcat
     4141 ctggccggtg gtggcgtccc tgacgccgat atcgaagcgc tcacagccca tggccttgag
     4201 ctgtcggcct atggcctgca aagtcctgtc gttcttcatc gggccaccaa gcgattccca
     4261 cacattatac gagccggaag cataaagtgt agctagatcc gaaggatgag ccgggctgaa
     4321 tgatcgaccg agacaggccc tgcggggctg cacacgcgcc cccacccttc gggtaggggg
     4381 aaaggccgct aaagcggcta aaagcgctcc agcgtatttc tgcggggttt ggtgtggggt
     4441 ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg tggggcggtg tgtagcctag
     4501 cgcagcgaat agaccagcta tccggcctct ggccgggcat attgggcaag ggcagcagcg
     4561 ccccacaagg gcgctgataa ccgcgcctag tggattattc ttagataatc atggatggat
     4621 ttttccaaca ccccgccagc ccccgcccct gctgggtttg caggtttggg ggcgtgacag
     4681 ttattgcagg ggttcgtgac agttattgca ggggggcgtg acagttattg caggggttcg
     4741 tgacagttag ggcgcgccca gctgtctagg gcggcggatt tgtcctactc aggagagcgt
     4801 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
     4861 tttgatgcct ttaattaaac gttcgtaatc aagccacttc ctttttgcat tgacgcaggg
     4921 tgtcggaagg caactcgccg aacgcgctcc tatagttttc agcgaagcgt cccaaatgta
     4981 agaagccgta gtctagggct atctcagtta tactacgcac attggcactg ggatcgttca
     5041 agcaggcgcg gatgctttcg agcttgcggt tgcggatgta gttcttcggc gtggtgccgg
     5101 cgtgcttctc gaacaaattg tagagcgagc gtggactcat catcgccagc tccgctaacc
     5161 gctcaaggct gatattccgt ttgagattct cctcaatgaa ttgaacgact cgctcgaaag
     5221 acgggttacc tttgctgaaa atttcacggc tgacattgct gcccagcatt tcgagcagct
     5281 tggaagcgat gatccccgca tagtgctctt ggacccgagg catcgacttt gtatgttccg
     5341 cttcgtcaca aactaacccg agtagattga taaagccatc gagttgctgg agattgtgtc
     5401 gcgcggcgaa acggataccc tccctcggct tgtgccaatt gttgtcactg cacgcccgat
     5461 caaggaccac tgagggcaat ttaacgataa atttctcgca atcttctgaa taggtcaggt
     5521 cggcttggtc atccggattg agcagcaata gttcgcccgg cgcaaaatag tgctcctggc
     5581 catggccacg ccacaggcaa tggcctttga gtattatttg cagatgataa caggtctcta
     5641 atccaggcga gattaccctc acgctaccgc cgtagctgat tcgacacaga tcgaggcatc
     5701 cgaagattct gtggtgcagc ctgcctgccg ggcgcccgcc cttgggcagg cgaatagagt
     5761 gcgtaccgac atactggtta acataatcgg agactgcata gggctcggcg tggacgaaga
     5821 tctgactttt ctcgttcaat aagcaaaaat ccatagttca cggttctctt attttaatgt
     5881 gggctgcttg gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct cgacgtgatg
     5941 cgtatacggg ttacccccat tgccacgttg cgccatcctt tttgcaatca gtgaccactt
     6001 ttccaagcaa aaataacgcc aagcagaacg aagacgttct ttttaagaag cgagaacacc
     6061 agaagttcgt gctgtcgggg catggggcga cgaattggcg gataaagggg atctgctgga
     6121 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     6181 tcacattctt gcccgcctga tgaatgctca tccgtaatta cgtatggcaa tgaaagacgg
     6241 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     6301 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     6361 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     6421 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     6481 ggccaatatg gacaacttct tcgcccccgt tttcaccatg catgggaatt agcttgatct
     6541 gaccaacgac cggtagcgga gctatccaac ggcggtatac caggaaaaca cacagcaggt
     6601 acatcagaac agtaccatga ctgaagaaca aatagttttt tcctgatcca taaagcagaa
     6661 cggcctgctc catgacaaat ctggctcccc aactaatgcc ccatgcagcc agcataacca
     6721 gcataaagtg cagtgtccgg tttgataggg ataagtccag ccttgcaaga agcggataca
     6781 ggagtgcaaa aaatggctat ctctagtaag gcctacccct taggctttat gcaacctcta
     6841 ggatccgaat tcaaataatt ttgtttaact ttaagaagga gatatacata tgaaatccgc
     6901 tgaatatttg aacactttta gattgagaaa tctcggccta cctgtcatga acaatttgca
     6961 tgacatgtct aaggcgactc gcatatctgt tgaaacactt cggttgttaa tctatacagc
     7021 tgattttcgc tataggatct acactgtaga aaagaaaggc ccagagaaga gaatgagaac
     7081 catttaccaa ccttctcgag aacttaaagc cttacaagga tgggttctac gtaacatttt
     7141 agataaactg tcgtcatctc ctttttctat tggatttgaa aagcaccaat ctattttgaa
     7201 taatgctacc ccgcatattg gggcaaactt tatactgaat attgatttgg aggatttttt
     7261 cccaagttta actgctaaca aagtttttgg agtgttccat tctcttggtt ataatcgact
     7321 aatatcttca gttttgacaa aaatatgttg ttataaaaat ctgctaccac aaggtgctcc
     7381 atcatcacct aaattagcta atctaatatg ttctaaactt gattatcgta ttcagggtta
     7441 tgcaggtagt cggggcttga tatatacgag atatgccgat gatctcacct tatctgcaca
     7501 gtctatgaaa aaggttgtta aagcacgtga ttttttattt tctataatcc caagtgaagg
     7561 attggttatt aactcaaaaa aaacttgtat tagtgggcct cgtagtcaga ggaaagttac
     7621 aggtttagtt atttcacaag agaaagttgg gataggtaga gaaaaatata aagaaattag
     7681 agcaaagata catcatatat tttgcggtaa gtcttctgag atagaacacg ttaggggatg
     7741 gttgtcattt attttaagtg tggattcaaa aagccatagg agattaataa cttatattag
     7801 caaattagaa aaaaaatatg gaaagaaccc tttaaataaa gcgaagacct aacatttaac
     7861 tatgataaga ttccgtatgc gcacccttag cgagaggttt atcattaagg tcaacctctg
     7921 gatgttgttt cggcatcctg cattgaatct gagttactgt ctgttttcct gaattcaagt
     7981 gaagagcgag tgtgagccgt tgtagcttca acctgtccct tacgaatagc tcttcacccg
     8041 aattcaggaa acccgttttt tctgacgtaa gggtgcgcat acggaatctg atcgaaataa
     8101 ttttgtttaa ctttaaggag gtttggaatg aaccaaatcg tgaagttcac tgacgactct
     8161 ggcctggcgg ttcaagttac tccagacgat gttcgccgtt atatctgtga gaacgctact
     8221 gaaaaagagg tgggcctctt tctgcaactc tgtcagactc aacgtctgaa tccgtttgtg
     8281 aaagacgctt acctggtgaa atacggcggt gctccagctt ctatgattac ttcctatcaa
     8341 gtttttaacc gtcgcgcgtg tcgtgatgct aactatgatg gtatcaaatc tggtgtggtt
     8401 gttctgcgtg acggtgatgt tgtgcataaa cgtggtgctg cgtgctacaa aaaggcgggt
     8461 gaggagctca tcggtggttg ggcggaagtt cgctttaagg atggccgcga gactgcgtat
     8521 gctgaggtgg cgctcgacga ctattccacc ggcaaatcta attgggcgaa aatgccgggt
     8581 gttatgatcg aaaaatgcgc gaaggctgct gcttggcgcc tcgcgttccc ggacactttt
     8641 cagggcatgt acgctgcgga ggaaatggat caagcgcaac agccagaaca ggtgcgcgct
     8701 caggcggagc aaccagtgga tctccagcca atccgcgaac tcttcaagcc atattgcgaa
     8761 cacttcggca tcactccggc tgagggtatg actgctgttt gtggtgcggt gggcgctgaa
     8821 ggcatgcact ctatgaccga gcagcaagct cgccgtgctc gcgcttggat ggaggaagaa
     8881 atggctgcgc cagctgtgga agcggagtat gaggttgttg acgagggcga ggtgttttaa
     8941 ggatccagga gaatacacat ggccagcaga ggcgtaaaca aggttattct cgttggtaat
     9001 ctgggtcagg acccggaagt acgctacatg ccaaatggtg gcgcagttgc caacattacg
     9061 ctggctactt ccgaatcctg gcgtgataaa gcgaccggcg agatgaaaga gcagactgaa
     9121 tggcaccgcg ttgtgctgtt cggcaaactg gcagaagtgg ccagcgaata tctgcgtaaa
     9181 ggttctcagg tttatatcga aggtcagctg cgtacccgta aatggaccga tcaatccggt
     9241 caggatcgct acaccacaga agtcgtggtg aacgttggcg gcaccatgca gatgctgggt
     9301 ggtcgtcagg gtggtggcgc tccggcaggt ggtaatatcg gtggtggtca gccgcagggc
     9361 ggttggggtc agcctcagca gccgcagggt ggcaatcagt tcagcggcgg cgcgcagtct
     9421 cgcccgcagc agtccgctcc ggcagcgccg tctaacgagc cgccgatgga ctttgatgat
     9481 gacattccgt tctgatctag agtcgacctg caggcatgca agcttgcggc cgcgtcgtga
     9541 ctgggaaaac cctggcgact agtcttggac tcctgttgat agatccagta atgacctcag
     9601 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     9661 atccaggggt ccccaataat tacgatttaa atttgtgtct caaaatctct gatgttacat
     9721 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     9781 tacaaggggt gttatgagcc atattcagcg tgaaa
//
