LOCUS       sequence-48361-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Chromosome- and spindle-pole-derived signals generate an intrinsic 
            code for spindle position and orientation.
  JOURNAL   Nat Cell Biol. 2012 Feb 12;14(3):311-7. doi: 10.1038/ncb2440.
  PUBMED    22327364
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-02-12- 12"; volume: "14"; issue: "3"; 
            pages: "311-7"
COMMENT     Sequence Label: pTK23
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             299..319
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             332..376
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     misc_feature    395..447
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    571..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(608..627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     662..681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(836..857)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
       61 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
      121 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
      181 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
      241 cgagctgtac aagtccggag gggatcggtg gagctccacc ggtggcggcc gctctagaga
      301 gaatctttat tttcagggcg ccgccaaatt caaagaaacc gctgctgcta aattcgaacg
      361 ccagcacatg gacagcggag gtggaggtac tagtctcgag ctcaagcttc gaattctgca
      421 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat
      481 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      541 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      601 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      661 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
      721 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
      781 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
      841 ttggaacaag agtccactat taaagaacgt ggactcc
//
