LOCUS       sequence-483610-        9384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010 origin of replication plasmid containing Eco1 recombitron 
            with a SapI flanked stuffer in the ncRNA expressed by lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Gonzalez-Delgado A, Bonillo-Lopez L, Johnson MS, Knodlseder N, Ko 
            CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragon V, 
            Gralnick JA, Guell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, 
            van Pijkeren JP, Shipman SL
  TITLE     Genome editing of phylogenetically distinct bacteria using portable 
            retron-mediated recombineering
  JOURNAL   bioRxiv 2025.06.16.660010
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.16.660010"
COMMENT     Sequence Label: pREC1010lac-Eco1
FEATURES             Location/Qualifiers
     source          1..9384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             467..475
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      1921..2015
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2136..2155)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2147..2962
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            3129..3237
                     /note="incP origin of transfer"
     CDS             complement(3248..4099)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(4086..4925)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(5436..6407)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6514..6908
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(6946..7032)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7077..8159)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8121..8140
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8160..8237)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     8443..8462
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    8477..8498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8513..8518,8519..8536,8537..8543)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8547..8571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8597..8619
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tgggataggt agagaaaaat ataaagaaat tagagcaaag atacatcata tattttgcgg
       61 taagtcttct gagatagaac acgttagggg atggttgtca tttattttaa gtgtggattc
      121 aaaaagccat aggagattaa taacttatat tagcaaatta gaaaaaaaat atggaaagaa
      181 ccctttaaat aaagcgaaga cctaacattt aactatgata agattccgta tgcgcaccct
      241 tagcgagagg tttatcatta aggtcaacct ctggatgttg tttcggcatc ctgcattgaa
      301 tctgagttac tgtctgtttt cctgaattca agtgaagagc gagtgtgagc cgttgtagct
      361 tcaacctgtc ccttacgaat agctcttcac ccgaattcag gaaacccgtt ttttctgacg
      421 taagggtgcg catacggaat ctgatcgaaa taattttgtt taactttaag gaggtttgga
      481 atgaaccaaa tcgtgaagtt cactgacgac tctggcctgg cggttcaagt tactccagac
      541 gatgttcgcc gttatatctg tgagaacgct actgaaaaag aggtgggcct ctttctgcaa
      601 ctctgtcaga ctcaacgtct gaatccgttt gtgaaagacg cttacctggt gaaatacggc
      661 ggtgctccag cttctatgat tacttcctat caagttttta accgtcgcgc gtgtcgtgat
      721 gctaactatg atggtatcaa atctggtgtg gttgttctgc gtgacggtga tgttgtgcat
      781 aaacgtggtg ctgcgtgcta caaaaaggcg ggtgaggagc tcatcggtgg ttgggcggaa
      841 gttcgcttta aggatggccg cgagactgcg tatgctgagg tggcgctcga cgactattcc
      901 accggcaaat ctaattgggc gaaaatgccg ggtgttatga tcgaaaaatg cgcgaaggct
      961 gctgcttggc gcctcgcgtt cccggacact tttcagggca tgtacgctgc ggaggaaatg
     1021 gatcaagcgc aacagccaga acaggtgcgc gctcaggcgg agcaaccagt ggatctccag
     1081 ccaatccgcg aactcttcaa gccatattgc gaacacttcg gcatcactcc ggctgagggt
     1141 atgactgctg tttgtggtgc ggtgggcgct gaaggcatgc actctatgac cgagcagcaa
     1201 gctcgccgtg ctcgcgcttg gatggaggaa gaaatggctg cgccagctgt ggaagcggag
     1261 tatgaggttg ttgacgaggg cgaggtgttt taaggatcca ggagaataca catggccagc
     1321 agaggcgtaa acaaggttat tctcgttggt aatctgggtc aggacccgga agtacgctac
     1381 atgccaaatg gtggcgcagt tgccaacatt acgctggcta cttccgaatc ctggcgtgat
     1441 aaagcgaccg gcgagatgaa agagcagact gaatggcacc gcgttgtgct gttcggcaaa
     1501 ctggcagaag tggccagcga atatctgcgt aaaggttctc aggtttatat cgaaggtcag
     1561 ctgcgtaccc gtaaatggac cgatcaatcc ggtcaggatc gctacaccac agaagtcgtg
     1621 gtgaacgttg gcggcaccat gcagatgctg ggtggtcgtc agggtggtgg cgctccggca
     1681 ggtggtaata tcggtggtgg tcagccgcag ggcggttggg gtcagcctca gcagccgcag
     1741 ggtggcaatc agttcagcgg cggcgcgcag tctcgcccgc agcagtccgc tccggcagcg
     1801 ccgtctaacg agccgccgat ggactttgat gatgacattc cgttctgatc tagagtcgac
     1861 ctgcaggcat gcaagcttgc ggccgcgtcg tgactgggaa aaccctggcg actagtcttg
     1921 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     1981 tcggttgccg ccgggcgttt tttattggtg agaatccagg ggtccccaat aattacgatt
     2041 taaatttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2101 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2161 gcgtgaaacg agctgtagcc gtccgcgtct gaacagcaac atggatgcgg atctgtatgg
     2221 ctataaatgg gcgcgtgata acgtgggtca gagcggcgcg accatttatc gtctgtatgg
     2281 caaaccggat gcgccggaac tgtttctgaa acatggcaaa ggcagcgtgg cgaacgatgt
     2341 gaccgatgaa atggtgcgtc tgaactggct gaccgaattt atgccgctgc cgaccattaa
     2401 acattttatt cgcaccccgg atgatgcgtg gctgctgacc accgcgattc cgggcaaaac
     2461 cgcgtttcag gtgctggaag aatatccgga tagcggcgaa aacattgtgg atgcgctggc
     2521 cgtgtttctg cgtcgtctgc atagcattcc ggtgtgcaac tgcccgttta acagcgatcg
     2581 tgtgtttcgt ctggcccagg cgcagagccg tatgaacaac ggcctggtgg atgcgagcga
     2641 ttttgatgat gaacgtaacg gctggccggt ggaacaggtg tggaaagaaa tgcataaact
     2701 gctgccgttt agcccggata gcgtggtgac ccacggcgat tttagcctgg ataacctgat
     2761 tttcgatgaa ggcaaactga ttggctgcat tgatgtgggc cgtgtgggca ttgcggatcg
     2821 ttatcaggat ctggccattc tgtggaactg cctgggcgaa tttagcccga gcctgcaaaa
     2881 acgtctgttt cagaaatatg gcattgataa tccggatatg aacaaactgc aatttcatct
     2941 gatgctggat gaatttttct aataattaat tggaccgcgg tccgcgcgtt gtccttttcc
     3001 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     3061 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     3121 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     3181 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag
     3241 gccggcctca gcctgccgcc ttgggccggg tgatgtcgta cttgcccgcc gcgaactcgg
     3301 ttaccgtcca gcccagcgcg accagctccg gcaacgcctc gcgcacccgc tggcggcgct
     3361 tgcgcatggt cgaaccactg gcctctgacg gccagacata gccgcacaag gtatctatgg
     3421 aagccttgcc ggttttgccg gggtcgatcc agccacacag ccgctggtgc agcaggcggg
     3481 cggtttcgct gtccagcgcc cgcacctcgt ccatgctgat gcgcacatgc tggccgccac
     3541 ccatgacggc ctgcgcgatc aaggggttca gggccacgta caggcgcccg tccgcctcgt
     3601 cgctggcgta ctccgacagc agccgaaacc cctgccgctt gcggccattc tgggcgatga
     3661 tggatacctt ccaaaggcgc tcgatgcagt cctgtatgtg cttgagcgcc ccaccactat
     3721 cgacctctgc cccgatttcc tttgccagcg cccgatagct acctttgacc accatggcat
     3781 cagcggtgac ggcctcccac ttgggttcca ggaacagccg gagctgccgt ccgccttcgg
     3841 tcttgggttc cgggccaagc actaggccat taggcccagc catggccacc agcccttgca
     3901 ggatgcgcag atcatcagcg cccagcggct ccgggccgct gaactcgatc cgcttgccgt
     3961 cgccgtagtc atacgtcacg tccagcttgc tgcgcttgcg ctcgccccgc ttgagggcac
     4021 ggaacaggcc gggggccaga cagtgcgccg ggtcgtgccg gacgtggctg aggctgtgct
     4081 tgttcttagg cttcaccacg gggcaccccc ttgctcttgc gctgcctctc cagcacggcg
     4141 ggcttgagca ccccgccgtc atgccgcctg aaccaccgat cagcgaacgg tgcgccatag
     4201 ttggccttgc tcacaccgaa gcggacgaag aaccggcgct ggtcgtcgtc cacaccccat
     4261 tcctcggcct cggcgctggt catgctcgac aggtaggact gccagcggat gttatcgacc
     4321 agtaccgagc tgccccggct ggcctgctgc tggtcgcctg cgcccatcat ggccgcgccc
     4381 ttgctggcat ggtgcaggaa cacgatagag cacccggtat cggcggcgat ggcctccatg
     4441 cgaccgatga cctgggccat ggggccgctg gcgttttctt cctcgatgtg gaaccggcgc
     4501 agcgtgtcca gcaccatcag gcggcggccc tcggcggcgc gcttgaggcc gtcgaaccac
     4561 tccggggcca tgatgttggg caggctgccg atcagcggct ggatcagcag gccgtcagcc
     4621 acggcttgcc gttcctcggc gctgaggtgc gccccaaggg cgtgcaggcg gtgatgaatg
     4681 gcggtgggcg ggtcttcggc gggcaggtag atcaccgggc cggtgggcag ttcgcccacc
     4741 tccagcagat ccggcccgcc tgcaatctgt gcggccagtt gcagggccag catggattta
     4801 ccggcaccac cgggcgacac cagcgccccg accgtaccgg ccaccatgtt gggcaaaacg
     4861 tagtccagcg gtggcggcgc tgctgcgaac gcctccagaa tattgatagg cttatgggta
     4921 gccattgatt gcctcctttg caggcagttg gtggttaggc gctggcgggg tcactacccc
     4981 cgccctgcgc cgctctgagt tcttccaggc actcgcgcag cgcctcgtat tcgtcgtcgg
     5041 tcagccagaa cttgcgctga cgcatccctt tggccttcat gcgctcggca tatcgcgctt
     5101 ggcgtacagc gtcagggctg gccagcaggt cgccggtctg cttgtccttt tggtctttca
     5161 tatcagtcac cgagaaactt gccggggccg aaaggcttgt cttcgcggaa caaggacaag
     5221 gtgcagccgt caaggttaag gctggccata tcagcgactg aaaagcggcc agcctcggcc
     5281 ttgtttgacg tataaccaaa gccaccgggc aaccaatagc ccttgtcact tttgatcagg
     5341 tagaccgacc ctgaagcgct tttttcgtat tccataaaac ccccttctgt gcgtgagtac
     5401 tcatagtata acaggcgtga gtaccaacgc aagcactaca tgctgaaatc tggcccgccc
     5461 ctgtccatgc ctcgctggcg gggtgccggt gcccgtgcca gctcggcccg cgcaagctgg
     5521 acgctgggca gacccatgac cttgctgacg gtgcgctcga tgtaatccgc ttcgtggccg
     5581 ggcttgcgct ctgccagcgc tgggctggcc tcggccatgg ccttgccgat ttcctcggca
     5641 ctgcggcccc ggctggccag cttctgcgcg gcgataaagt cgcacttgct gaggtcatca
     5701 ccgaagcgct tgaccagccc ggccatctcg ctgcggtact cgtccagcgc cgtgcgccgg
     5761 tggcggctaa gctgccgctc gggcagttcg aggctggcca gcctgcgggc cttctcctgc
     5821 tgccgctggg cctgctcgat ctgctggcca gcctgctgca ccagcgccgg gccagcggtg
     5881 gcggtcttgc ccttggattc acgcagcagc acccacggct gataaccggc gcgggtggtg
     5941 tgcttgtcct tgcggttggt gaagcccgcc aagcggccat agtggcggct gtcggcgctg
     6001 gccgggtcgg cgtcgtactc gctggccagc gtccgggcaa tctgcccccg aagttcaccg
     6061 cctgcggcgt cggccacctt gacccatgcc tgatagttct tcgggctggt ttccactacc
     6121 agggcaggct cccggccctc ggctttcatg tcatccaggt caaactcgct gaggtcgtcc
     6181 accagcacca gaccatgccg ctcctgctcg gcgggcctga tatacacgtc attgccctgg
     6241 gcattcatcc gcttgagcca tggcgtgttc tggagcactt cggcggctga ccattcccgg
     6301 ttcatcatct ggccggtggt ggcgtccctg acgccgatat cgaagcgctc acagcccatg
     6361 gccttgagct gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg gccaccaagc
     6421 gattcccaca cattatacga gccggaagca taaagtgtag ctagatccga aggatgagcc
     6481 gggctgaatg atcgaccgag acaggccctg cggggctgca cacgcgcccc cacccttcgg
     6541 gtagggggaa aggccgctaa agcggctaaa agcgctccag cgtatttctg cggggtttgg
     6601 tgtggggttt agcgggcttt gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg
     6661 tagcctagcg cagcgaatag accagctatc cggcctctgg ccgggcatat tgggcaaggg
     6721 cagcagcgcc ccacaagggc gctgataacc gcgcctagtg gattattctt agataatcat
     6781 ggatggattt ttccaacacc ccgccagccc ccgcccctgc tgggtttgca ggtttggggg
     6841 cgtgacagtt attgcagggg ttcgtgacag ttattgcagg ggggcgtgac agttattgca
     6901 ggggttcgtg acagttaggg cgcgcccagc tgtctagggc ggcggatttg tcctactcag
     6961 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
     7021 tcgttttatt tgatgccttt aattaaacgt tcgtaatcaa gccacttcct ttttgctcac
     7081 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7141 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     7201 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     7261 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     7321 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     7381 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     7441 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     7501 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     7561 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     7621 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     7681 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     7741 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     7801 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     7861 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     7921 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     7981 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     8041 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     8101 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     8161 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     8221 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     8281 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     8341 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     8401 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     8461 ggcgccagca accgcataat gtgagttagc tcactcatta ggcaccccag gctttacact
     8521 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattc ccctctagga
     8581 tccgaattca aataattttg tttaacttta agaaggagat atacatatga aatccgctga
     8641 atatttgaac acttttagat tgagaaatct cggcctacct gtcatgaaca atttgcatga
     8701 catgtctaag gcgactcgca tatctgttga aacacttcgg ttgttaatct atacagctga
     8761 ttttcgctat aggatctaca ctgtagaaaa gaaaggccca gagaagagaa tgagaaccat
     8821 ttaccaacct tctcgagaac ttaaagcctt acaaggatgg gttctacgta acattttaga
     8881 taaactgtcg tcatctcctt tttctattgg atttgaaaag caccaatcta ttttgaataa
     8941 tgctaccccg catattgggg caaactttat actgaatatt gatttggagg attttttccc
     9001 aagtttaact gctaacaaag tttttggagt gttccattct cttggttata atcgactaat
     9061 atcttcagtt ttgacaaaaa tatgttgtta taaaaatctg ctaccacaag gtgctccatc
     9121 atcacctaaa ttagctaatc taatatgttc taaacttgat tatcgtattc agggttatgc
     9181 aggtagtcgg ggcttgatat atacgagata tgccgatgat ctcaccttat ctgcacagtc
     9241 tatgaaaaag gttgttaaag cacgtgattt tttattttct ataatcccaa gtgaaggatt
     9301 ggttattaac tcaaaaaaaa cttgtattag tgggcctcgt agtcagagga aagttacagg
     9361 tttagttatt tcacaagaga aagt
//
