LOCUS       sequence-48370-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Chromosome- and spindle-pole-derived signals generate an intrinsic 
            code for spindle position and orientation.
  JOURNAL   Nat Cell Biol. 2012 Feb 12;14(3):311-7. doi: 10.1038/ncb2440.
  PUBMED    22327364
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-02-12- 12"; volume: "14"; issue: "3"; 
            pages: "311-7"
COMMENT     Sequence Label: pTK24
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             294..314
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             327..371
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
       61 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
      121 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
      181 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
      241 tgtacaagtc cggaggggat cggtggagct ccaccggtgg cggccgctct agagagaatc
      301 tttattttca gggcgccgcc aaattcaaag aaaccgctgc tgctaaattc gaacgccagc
      361 acatggacag cggaggtgga ggtactagtc tcgagatgag tgctgcagtg actgcaggga
      421 agctggcacg ggcaccggcc gaccctggga aagccggggt ccccggagtt gcagctcccg
      481 gagctccggc ggcggctcca ccggcgaaag agatcccgga ggtcctagtg gacccacgca
      541 gccggcggcg ctatgtgcgg ggccgctttt tgggcaaggg cggctttgcc aagtgcttcg
      601 agatctcgga cgcggacacc aaggaggtgt tcgcgggcaa gattgtgcct aagtctctgc
      661 tgctcaagcc gcaccagagg gagaagatgt ccatggaaat atccattcac cgcagcctcg
      721 cccaccagca cgtcgtagga ttccacggct ttttcgagga caacgacttc gtgttcgtgg
      781 tgttggagct ctgccgccgg aggtctctcc tggagctgca caagaggagg aaagccctga
      841 ctgagcctga ggcccgatac tacctacggc aaattgtgct tggctgccag tacctgcacc
      901 gaaaccga
//
