LOCUS       sequence-48372-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Chromosome- and spindle-pole-derived signals generate an intrinsic 
            code for spindle position and orientation.
  JOURNAL   Nat Cell Biol. 2012 Feb 12;14(3):311-7. doi: 10.1038/ncb2440.
  PUBMED    22327364
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2012-02-12- 12"; volume: "14"; issue: "3"; 
            pages: "311-7"
COMMENT     Sequence Label: pTK25
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             296..316
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             329..373
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
       61 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      121 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      181 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      241 gctgtacaag tccggagggg atcggtggag ctccaccggt ggcggccgct ctagagagaa
      301 tctttatttt cagggcgccg ccaaattcaa agaaaccgct gctgctaaat tcgaacgcca
      361 gcacatggac agcggaggtg gaggtactag tctcgagatg agtgctgcag tgactgcagg
      421 gaagctggca cgggcaccgg ccgaccctgg gaaagccggg gtccccggag ttgcagctcc
      481 cggagctccg gcggcggctc caccggcgaa agagatcccg gaggtcctag tggacccacg
      541 cagccggcgg cgctatgtgc ggggccgctt tttgggcaag ggcggctttg ccaagtgctt
      601 cgagatctcg gacgcggaca ccaaggaggt gttcgcgggc aggattgtgc ctaagtctct
      661 gctgctcaag ccgcaccaga gggagaagat gtccatggaa atatccattc accgcagcct
      721 cgcccaccag cacgtcgtag gattccacgg ctttttcgag gacaacgact tcgtgttcgt
      781 ggtgttggag ctctgccgcc ggaggtctct cctggagctg cacaagagga ggaaagccct
      841 gactgagcct gaggcccgat actacctacg gcaaattgtg cttggctgc
//
