LOCUS       sequence-483727-        4241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  riboprobes (SP6 or T3) and dsRNA (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Rozario T, Quinn EB, Wang J, Davis RE, Newmark PA
  TITLE     Region-specific regulation of stem cell-driven regeneration in 
            tapeworms.
  JOURNAL   eLife. 2019 Sep 24;8. pii: 48958. doi: 10.7554/eLife.48958.
  PUBMED    31549962
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-09-24"; volume: "8. pii: 48958. doi"; pages: " 
            10.7554/eLife.48958"
COMMENT     Sequence Label: TR29_M6_cel135
FEATURES             Location/Qualifiers
     source          1..4241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2..49)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        85..103
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1121..1139)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1122..1139)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1143..1161)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1175..1222
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1234..1251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1405..1993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1485..1504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2164..2955,2956..3024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2787..2806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3042..3836)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3153..3172)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3763..3782
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagg cgcgccggat
       61 cctaatacga ctcactatag ggagaattaa ccctcactaa agggagacct ttgcattctg
      121 ttgacccaga ttttgtacct gtaattcagc tctgctcaga gcagacttgc actgcatcaa
      181 ctcttgatct tttccttcaa gagtgtctcg tagccgatcg acctcagctg aacgttcatc
      241 actttggaga cgcgcgttgg ctgcagccgt ttctgcgtct gtccatctag cacgccagac
      301 ctccgcatcc tctgcagctg cacgggcttg ttcggctagt agatcacgtt ctcgagctct
      361 ctcatccaat aaacgttcct gtctggctag ttcagttaaa tagccgtcag ctcgtgtctt
      421 cagcgcttct ctgtcgtctt ctgtattgtg caactccgca gtcagtcgct gaatgtttcg
      481 cacagcagat gtgagttcat ctcgcaattt gagtgtttcc cgttccccag tatctagcgc
      541 ccttcgactt tcacgaatgc tactttgagc tgcagccagt tcacttctca atgactgctc
      601 agatgcttgt atcgtcatta gctgctcctc ttttcgggag agcattcgag tattttcctc
      661 tagagagcta cgagtctctt tgatttccat cagttgagaa gttatttgtg actcaagttc
      721 agatatcctt tgagccatca gcttccgatt agcctctccg gcatctcttt gttcttccat
      781 agcttcaaga cgagacctga cctgcaagta gtcatcgtgt tccattctta actcccgctc
      841 tagttgagtg gctcggatct ccgcttgatg gaatcgctct tcagcatttc ggtgtttgtt
      901 caccatttct gccagttcag tacttagttc ttcgcatcgc tgttttattg aatcattttc
      961 tttgcgcaaa tcaacgacag attgatgctc cttaatagca acatcggttt ggcgtttaac
     1021 atctgcttct atggttctca agtcagactc caattcggca atcttttctc gagcaagagt
     1081 atgacgatgt gtacaatcat cccgatctcg aaaggtcact tctatagtgt cacctaaatc
     1141 tccctatagt gagtcgtatt aggatccggc gcgcctagca taaccccttg gggcctctaa
     1201 acgggtcttg aggggttttt tgacgcgtgc ttcctcgctc actgactcgc tgcgctcggt
     1261 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1321 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1381 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1441 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1501 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1561 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1621 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1741 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1801 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1861 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1921 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1981 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2041 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2101 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2161 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2221 catagttgcc tggctccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2281 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2341 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2401 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2461 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2521 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2581 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2641 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2701 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2761 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2821 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2881 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2941 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3001 gacacggaaa tgttgaatac tcatactctt cctttttcaa ttcagaagaa ctcgtcaaga
     3061 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     3121 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     3181 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     3241 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     3301 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     3361 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     3421 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     3481 gcatcagcca tgatggatac tttctcggca ggagcaaggt gggatgacag gagatcctgc
     3541 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     3601 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     3661 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     3721 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     3781 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     3841 aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatcattggc
     3901 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
     3961 gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta
     4021 agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc
     4081 cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc
     4141 cgcttccttt agcagccctt gcgccctgaa ttttgttaaa attcgcgtta aatttttgtt
     4201 aaatcagctc attttttaac caataggccg aaatcgacgc g
//
