LOCUS       sequence-48374-e         474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Yamamoto Y, Takeshita H, Sawa H
  TITLE     Multiple Wnts redundantly control polarity orientation in 
            Caenorhabditis elegans epithelial stem cells.
  JOURNAL   PLoS Genet. 2011 Oct;7(10):e1002308. Epub 2011 Oct 13.
  PUBMED    22022276
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2011-10"; volume: "7(10)"; pages: "e1002308. Epub 
            2011 Oct 13"
COMMENT     Sequence Label: pPD95.77-wVenus
FEATURES             Location/Qualifiers
     source          1..474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..26)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15..31
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(39..45,46..63,64..69))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    84..105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(222..239)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
       61 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      121 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      181 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      241 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      301 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      361 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      421 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataa
//
