LOCUS       sequence-483799-       14173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EGFP and mRFP1 simultaneously in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14173)
  AUTHORS   Guoliang Yuan, Tatiana Serrano-Zayas, Ziyu Dai, Erica I. DeMers, 
            Shuang Deng, Beth A. Hofstad,, Kyle R. Pomraning
  TITLE     Strong Translation Elongation Factor 1-Alpha (tef1-alpha) Promoter 
            Reporter Systems for Aspergillus niger
  JOURNAL   ACS Synthetic Biology Article ASAP
REFERENCE   2  (bases 1 to 14173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology Article ASAP"
COMMENT     Sequence Label: pGY214
FEATURES             Location/Qualifiers
     source          1..14173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1144..1146,1147..1860)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1144..1146,1147..1857)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1186..1207)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1447..1466)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1794..1815
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3242..3916
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     CDS             3242..3916
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(3369..3387)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3637..3656
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3831..3854
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4739..5764
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(5836..5869)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(6339..6355)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6454..6471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6625..7213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6705..6724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7335..8126,8127..8195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7958..7977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8196..8287)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8329..8348
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8354..8373
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    8580..8613
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      8926..14173
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aaggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1201 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1261 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1321 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1381 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1441 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1501 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1561 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1621 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1681 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1741 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1801 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     1861 gcggacattc gatttatgcc gttatgactt ccttaaaaaa gcctttacga atgaaagaaa
     1921 tggaattaga cttgttatgt agttgattct acaatggatt atgattcctg aacttcaaat
     1981 ccgctgttca ttattaatct cagctcttcc cgtaaagcca atgttgaaac tattcgtaaa
     2041 tgtacctcgt tttgcgtgta ccttgcttat cacgtgatat tacatgacct ggacagagtt
     2101 ctgcgcgaaa gtcataacgt aaatcccggg cggtaggtgc gtcccgggcg gaaggtagtt
     2161 ttctcgtcca ccccaacgcg tttatcaacc tcaactttca acaaccatca tgccaccaaa
     2221 agcgcgtaaa acaaagcgag atttgattga gcaagagggc aggatccaat gcgcgattca
     2281 agacattaaa aatggaaaat ttcaaaaaat tgcgcccgca gcgcgtgcat acaaaattca
     2341 tcccaataca agtgtaatgc tagtggaggt ccgttgacct cacagggatt tccaccaaaa
     2401 agtggaccgg gcgggcgcca ctcggccgtg ccacagcagc ctgagagcgg acaaataaca
     2461 acagccgcct gccgcggggt tcggttgcaa acatgaccaa caggccaggc catcatcaac
     2521 ccaccgctgc gttgatgccc aggatttcag tccaataatc cacaatttac caacggatag
     2581 agctaggtga actagataga caggagggcc agagggaggg gaccgagatg aaaaattttc
     2641 gatgaaagag tggtcaaggt ggggtcgtag ttcggcgctc cgagggcgag gaaccaagga
     2701 aaggcgagaa aaggacaggc tgatcgcgct gcgttgctgg gctgcaagcg tgtccagttg
     2761 agtctggaaa aggctccgcc gtgaagattc tgcgttggtc ccgcacctgc gcggtggggg
     2821 cattacccct ccatgtccaa tgatttcaag tcaaagccaa gggttgaagc ccgcccgctt
     2881 agtcgccttc tcgcttgacc cctccatata agtatttccc ctcctccccc tcccacaaat
     2941 ttttcctttc cctttcctcc ctcgtccgct tcagtacgta tatcttcccc ccctctctct
     3001 tccttctcac tcttctctcc ttctttcttg attcatcctc tctctaactg acttctttgc
     3061 tcagcacctc tacgcgttct ggccgtagta tctgagcaat ttttctacag actttttcta
     3121 tctaattcca aaaaagaact tcgagttcat tcacaaccgt catcatgggg tgagtcaaga
     3181 caacgatcat catctgacaa aggaaggaca tgggctaacg gatatcttct acaacagtaa
     3241 ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
     3301 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3361 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     3421 gtcccctcag ttccagtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3481 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3541 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3601 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3661 gggctgggag gcctccaccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3721 caagatgagg ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta
     3781 catggccaag aagcccgtgc agctgcccgg cgcctacaag accgacatca agctggacat
     3841 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca
     3901 ctccaccggc gcctaagcgg acattcgatt tatgccgtta tgacttcctt aaaaaagcct
     3961 ttacgaatga aagaaatgga attagacttg ttatgtagtt gattctacaa tggattatga
     4021 ttcctgaact tcaaatccgc tgttcattat taatctcagc tcttcccgta aagccaatgt
     4081 tgaaactatt cgtaaatgta cctcgttttg cgtgtacctt gcttatcacg tgatattaca
     4141 tgacctggac agagttctgc gcgaaagtca taacgtaaat cccgggcggt aggtgcgtcc
     4201 cgggcggaag gtagttttct cgtccacccc aacgcgttta tcaacctcaa ctttcaacaa
     4261 ccatcatgcc accaaaagcg cgtaaaacaa agcgagattt gattgagcaa gagggcagga
     4321 tccaatgcgc gattcaagac attaaaaatg gaaaatttca aaaaattgcg cccgcagcgc
     4381 gtgcatacaa aattcatccc aatacaagtg taatgctagt ggaggtcaac acatcaatgc
     4441 ctattttggt ttagtcgtcc aggcggtgag cacaaaattt gtgtcgtttg acaagatggt
     4501 tcatttaggc aactggtcag atcagcccca cttgtagcag tagcggcggc gctcgaagtg
     4561 tgactcttat tagcagacag gaacgaggac attattatca tctgctgctt ggtgcacgat
     4621 aacttggtgc gtttgtcaag caaggtaagt ggacgacccg gtcatacctt cttaagttcg
     4681 cccttcctcc ctttatttca gattcaatct gacttaccta ttctacccaa gcatccaaat
     4741 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     4801 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     4861 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     4921 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     4981 ggagttcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     5041 agacctgcct gaaaccgaac tgcccgctgt tctccagccg gtcgcggagg ccatggatgc
     5101 gattgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     5161 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     5221 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     5281 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcatgcgg atttcggctc
     5341 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     5401 gttcggggat tcccaatacg aggtcgccaa catcctcttc tggaggccgt ggttggcttg
     5461 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     5521 cctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     5581 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     5641 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     5701 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     5761 atagagtaga tgccgaccga tccccactac cgcattaaga cctcagcgcg gccgcaaatt
     5821 taaataaaat gaagtgaagt tcctatactt tctagagaat aggaacttct atagtgagtc
     5881 gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag
     5941 tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt
     6001 ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta
     6061 tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa
     6121 aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc
     6181 tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa
     6241 actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt
     6301 actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac
     6361 agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat
     6421 gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt
     6481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6541 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6841 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6961 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7021 tacagagttc ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat
     7081 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7141 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7201 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7261 cgcgcgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca
     7321 gcgtaatgct ctgcttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7441 ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc ggctccggat
     7501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7621 aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt
     7681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7801 gcagtgttat cactcatggt tatggcagcg ctacataatt ctcttactgt catgccatcc
     7861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8161 ggaataaggg cgacacggaa atgttgaata ctcatattct tcctttttca atattattga
     8221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8281 aaacaaatag gggtcagtgt tacaaccaat taaccaattc tgaacattat cgcgagccca
     8341 tttatacctg aatatggctc ataacacccc ttgtttgcct ggcggcagta gcgcggtggt
     8401 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
     8461 gactccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     8521 aagactgggc ctttcgcccg ggctaattat ggggtgtcgc ccttattcga ctctatagtg
     8581 aagttcctat tctctagaaa gtataggaac ttctgaagtg gggatttaaa tgcggccgcg
     8641 ctgagggttt aatcgacgaa gcagctgacg gccagtgcca agcttaacgc gtaccgggcc
     8701 cagtatatgt tccgcagatg actggagctc tgccatacgt gccctctcaa gcaccatttg
     8761 ttccatctac agagactagt caccaactag tctatcaaga ctcacagggt acattgctga
     8821 gaccaactga ccagaggcag ggtagcggat tgacggctcc atctccttca cttacaaggt
     8881 ctattgaaag ccctttagca tcaccaagcg gagaatagat tgttaagctt attttttgta
     8941 tactgttttg tgatagcacg aagtttttcc acggtatctt gttaaaaata tatatttgtg
     9001 gcgggcttac ctacatcaaa ttaataagag actaattata aactaaacac acaagcaagc
     9061 tactttaggg taaaagttta taaatgcttt tgacgtataa acgttgcttg tatttattat
     9121 tacaattaaa ggtggataga aaacctagag actagttaga aactaatctc aggtttgcgt
     9181 taaactaaat cagagcccga gaggttaaca gaacctagaa ggggactaga tatccgggta
     9241 gggaaacaaa aaaaaaaaca agacagccac atattaggga gactagttag aagctagttc
     9301 caggactagg aaaataaaag acaatgatac cacagtctag ttgacaacta gatagattct
     9361 agattgaggc caaagtctct gagatccagg ttagttgcaa ctaatactag ttagtatcta
     9421 gtctcctata actctgaagc tagaataact tactactatt atcctcacca ctgttcagct
     9481 gcgcaaacgg agtgattgca aggtgttcag agactagtta ttgactagtc agtgactagc
     9541 aataactaac aaggtattaa cctaccatgt ctgccatcac cctgcacttc ctcgggctca
     9601 gcagcctttt cctcctcatt ttcatgctca ttttccttgt ttaagactgt gactagtcaa
     9661 agactagtcc agaaccacaa aggagaaatg tcttaccact ttcttcattg cttgtctctt
     9721 ttgcattatc catgtctgca actagttaga gtctagttag tgactagtcc gacgaggact
     9781 tgcttgtctc cggattgttg gaggaactct ccagggcctc aagatccaca acagagcctt
     9841 ctagaagact ggtcaataac tagttggtct ttgtctgagt ctgacttacg aggttgcata
     9901 ctcgctccct ttgcctcgtc aatcgatgag aaaaagcgcc aaaactcgca atatggcttt
     9961 gaaccacacg gtgctgagac tagttagaat ctagtcccaa actagcttgg atagcttacc
    10021 tttgcccttt gcgttgcgac aggtcttgca gggtatggtt cctttctcac cagctgattt
    10081 agctgccttg ctaccctcac ggcggatctg cataaagagt ggctagaggt tataaattag
    10141 cactgatcct aggtacgggg ctgaatgtaa cttgccyttc ctttctcatc gcgcggcaag
    10201 acaggcttgc tcaaattcct accagtcaca ggggtatgca cggcgtacgg accacttgaa
    10261 ctagtcacag attagttagc aactagtctg cattgaatgg ctgtacttac gggccctcgc
    10321 cattgtcctg atcatttcca gcttcaccct cgttgctgca aagtagttag tgactagtca
    10381 aggactagtt gaaatgggag aagaaactca cgaattctcg acacccttag tattgtggtc
    10441 cttggacttg gtgctgctat atattagcta atacactagt tagactcaca gaaacttacg
    10501 cagctcgctt gcgcttcttg gtaggagtcg gggttgggag aacagtgcct tcaaacaagc
    10561 cttcatacca tgctacttga ctagtcaggg actagtcacc aagtaatcta gataggactt
    10621 gcctttggcc tccatcagtt ccttcatagt gggaggtcca ttgtgcaatg taaactccat
    10681 gccgtgggag ttcttatcct tcaagtgctt gaccaatatg tttctgttgg cagagggaac
    10741 ctgtcaacta gttaataact agtcagaaac tagtatagca gtagactcac tgtacgcttg
    10801 aggcatccct tcactcggca gtagacttca tatggatgga tatcaggcac gccattgtcg
    10861 tcctgtggac tagtcagtaa ctaggcttaa agctagtcgg gtcggcttac tatcttgaaa
    10921 tccggcagcg taagctcccc gtccttaact gcctcgagat agtgacagta ctctggggac
    10981 tttcggagat cgttatcgcg aatgctcggc atactaatcg ttgactagtc ttggactagt
    11041 cccgagcaaa aaggattgga ggaggaggag gaaggtgaga gtgagacaaa gagcgaaata
    11101 agagcttcaa aggctatctc taagcagtat gaaggttaag tatctagttc ttgactagat
    11161 ttaaaagaga tttcgactag ttatgtacct ggagtttgga tataggaatg tgttgtggta
    11221 acgaaatgta agggggagga aagaaaaagt cggtcaagag gtaactctaa gtcggccatt
    11281 cctttttggg aggcgctaac cataaacggc atggtcgact tagagttagc tcagggaatt
    11341 tagggagtta tctgcgacca ccgaggaacg gcggaatgcc aaagaatccc gatggagctc
    11401 tagctggcgg ttgacaaccc caccttttgg cgtttctgcg gcgttgcagg cgggactgga
    11461 tacttcgtag aaccagaaag gcaaggcaga acgcgctcag caagagtgtt ggaagtgata
    11521 gcatgatgtg ccttgttaac taggtcaaaa tctgcagtat gcttgatgtt atccaaagtg
    11581 tgagagagga aggtccaaac atacacgatt gggagagggc ctaggtataa gagtttttga
    11641 gtagaacgca tgtgagccca gccatctcga ggagattaaa cacgggccgg catttgatgg
    11701 ctatgttagt accccaatgg aaacggtgag agtccagtgg tcgcagataa ctccctaaat
    11761 tccctgagct aactctaagt cgaccatgcc gtttatggtt agcgcctccc aaaaaggaat
    11821 ggccgactta gagttacctc ttgaccgact ttttctttcc tcccccttac atttcgttac
    11881 cacaacacat tcctatatcc aaactccagg tacataacta gtcgaaatct cttttaaatc
    11941 tagtcaagaa ctagatactt aaccttcata ctgcttagag atagcctttg aagctcttat
    12001 ttcgctcttt gtctcactct caccttcctc ctcctcctcc aatccttttt gctcgggact
    12061 agtccaagac tagtcaacga ttagtatgcc gagcattcgc gataacgatc tccgaaagtc
    12121 cccagagtac tgtcactatc tcgaggcagt taaggacggg gagcttacgc tgccggattt
    12181 caagatagta agccgacccg actagcttta agcctagtta ctgactagtc cacaggacga
    12241 caatggcgtg cctgatatcc atccatatga agtctactgc cgagtgaagg gatgcctcaa
    12301 gcgtacagtg agtctactgc tatactagtt tctgactagt tattaactag ttgacaggtt
    12361 ccctctgcca acagaaacat attggtcaag cacttgaagg acaagaactc ccacggcatg
    12421 gagtttacat tgcacaatgg acctcccact atgaaggaac tgatggaggc caaaggcaag
    12481 tcctatctag attacttggt gactagtccc tgactagtca agtagcatgg tatgaaggct
    12541 tgtttgaagg cactgttctc ccaaccccga ctcctaccaa gaagcgcaag cgagctgcgt
    12601 aagtttctgt gagtctaact agtgtattag ctaatatata gcagcaccaa gtccaaggac
    12661 cacaatacta agggtgtcga gaattcgtga gtttcttctc ccatttcaac tagtccttga
    12721 ctagtcacta actactttgc agcaacgagg gtgaagctgg aaatgatcag gacaatggcg
    12781 agggcccgta agtacagcca ttcaatgcag actagttgct aactaatctg tgactagttc
    12841 aagtggtccg tacgccgtgc atacccctgt gactggtagg aatttgagca agcctgtctt
    12901 gccgcgcgat gagaaaggaa rggcaagtta cattcagccc cgtacctagg atcagtgcta
    12961 atttataacc tctagccact ctttatgcag atccgccgtg agggtagcaa ggcagctaaa
    13021 tcagctggtg agaaaggaac cataccctgc aagacctgtc gcaacgcaaa gggcaaaggt
    13081 aagctatcca agctagtttg ggactagatt ctaactagtc tcagcaccgt gtggttcaaa
    13141 gccatattgc gagttttggc gctttttctc atcgattgac gaggcaaagg gagcgagtat
    13201 gcaacctcgt aagtcagact cagacaaaga ccaactagtt attgaccagt cttctagaag
    13261 gctctgttgt ggatcttgag gccctggaga gttcctccaa caatccggag acaagcaagt
    13321 cctcgtcgga ctagtcacta actagactct aactagttgc agacatggat aatgcaaaag
    13381 agacaagcaa tgaagaaagt ggtaagacat ttctcctttg tggttctgga ctagtctttg
    13441 actagtcaca gtcttaaaca aggaaaatga gcatgaaaat gaggaggaaa aggctgctga
    13501 gcccgaggaa gtgcagggtg atggcagaca tggtaggtta ataccttgtt agttattgct
    13561 agtcactgac tagtcaataa ctagtctctg aacaccttgc aatcactccg tttgcgcagc
    13621 tgaacagtgg tgaggataat agtagtaagt tattctagct tcagagttat aggagactag
    13681 atactaacta gtattagttg caactaacct ggatctcaga gactttggcc tcaatctaga
    13741 atctatctag ttgtcaacta gactgtggta tcattgtctt ttattttcct agtcctggaa
    13801 ctagcttcta actagtctcc ctaatatgtg gctgtcttgt tttttttttt tgtttcccta
    13861 cccggatatc tagtcccctt ctaggttctg ttaacctctc gggctctgat ttagtttaac
    13921 gcaaacctga gattagtttc taactagtct ctaggttttc tatccacctt taattgtaat
    13981 aataaataca agcaacgttt atacgtcaaa agcatttata aacttttacc ctaaagtagc
    14041 ttgcttgtgt gtttagttta taattagtct cttattaatt tgatgtaggt aagcccgcca
    14101 caaatatata tttttaacaa gataccgtgg aaaaacttcg tgctatcaca aaacagtata
    14161 caaaaaataa gct
//
