LOCUS       sequence-483806-       12700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express H3-EGFP in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Guoliang Yuan, Tatiana Serrano-Zayas, Ziyu Dai, Erica I. DeMers, 
            Shuang Deng, Beth A. Hofstad,, Kyle R. Pomraning
  TITLE     Strong Translation Elongation Factor 1-Alpha (tef1-alpha) Promoter 
            Reporter Systems for Aspergillus niger
  JOURNAL   ACS Synthetic Biology Article ASAP
REFERENCE   2  (bases 1 to 12700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology Article ASAP"
COMMENT     Sequence Label: pGY220
FEATURES             Location/Qualifiers
     source          1..12700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1727..1729,1730..2443)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1727..1729,1730..2440)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1769..1790)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2030..2049)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2377..2398
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3266..4291
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4363..4396)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4866..4882)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4981..4998)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5152..5740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5232..5251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5862..6653,6654..6722))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6485..6504
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6723..6814)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6856..6875
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6881..6900
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    7107..7140
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      7453..12700
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aaggcccgca ctaagcagac tgcccgtaag tgcaacctca ctgtcctttc ccctcgtgtc
     1201 gttcgaccgc gtcatggtcg cgacacccgt gtctcacatg aggaacatca tcactaacta
     1261 tcatctcaat ataggtaagt ccactggtgg caaggctccc cgtaagcagc tcgcctccaa
     1321 ggctgcccgt aaggccgctc cctccaccgg aggtgtcaag aagcctcacc gttacaagcc
     1381 cggtatgtat acactcatca ttcaaagtgg caaatggcct atccgaacag tcaactaaca
     1441 gtctaccaaa ggtaccgtcg ctctgcgtga aatccgtcgt taccagaaga gcactgagct
     1501 cctgatccgc aagctgccct tccagcgtct tgtccgtgaa attgctcagg acttcaagtc
     1561 ggatctccgc ttccagtcct ccgccatcgg tgctcttcag gagtccgtcg aggcctacct
     1621 cgtctccctc ttcgaggaca ccaacctgtg cgccatccac gccaagcgtg tcaccatcca
     1681 gtccaaggac atccagcttg cccgccgtct ccgtggtgag cgctctgtga gcaagggcga
     1741 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1801 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1861 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1921 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1981 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2041 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2101 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2161 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2221 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2281 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2341 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2401 cgccgccggg atcactctcg gcatggacga gctgtacaag taagcggaca ttcgatttat
     2461 gccgttatga cttccttaaa aaagccttta cgaatgaaag aaatggaatt agacttgtta
     2521 tgtagttgat tctacaatgg attatgattc ctgaacttca aatccgctgt tcattattaa
     2581 tctcagctct tcccgtaaag ccaatgttga aactattcgt aaatgtacct cgttttgcgt
     2641 gtaccttgct tatcacgtga tattacatga cctggacaga gttctgcgcg aaagtcataa
     2701 cgtaaatccc gggcggtagg tgcgtcccgg gcggaaggta gttttctcgt ccaccccaac
     2761 gcgtttatca acctcaactt tcaacaacca tcatgccacc aaaagcgcgt aaaacaaagc
     2821 gagatttgat tgagcaagag ggcaggatcc aatgcgcgat tcaagacatt aaaaatggaa
     2881 aatttcaaaa aattgcgccc gcagcgcgtg catacaaaat tcatcccaat acaagtgtaa
     2941 tgctagtgga ggtcaacaca tcaatgccta ttttggttta gtcgtccagg cggtgagcac
     3001 aaaatttgtg tcgtttgaca agatggttca tttaggcaac tggtcagatc agccccactt
     3061 gtagcagtag cggcggcgct cgaagtgtga ctcttattag cagacaggaa cgaggacatt
     3121 attatcatct gctgcttggt gcacgataac ttggtgcgtt tgtcaagcaa ggtaagtgga
     3181 cgacccggtc ataccttctt aagttcgccc ttcctccctt tatttcagat tcaatctgac
     3241 ttacctattc tacccaagca tccaaatgaa aaagcctgaa ctcaccgcga cgtctgtcga
     3301 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     3361 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     3421 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     3481 cccgattccg gaagtgcttg acattgggga gttcagcgag agcctgacct attgcatctc
     3541 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     3601 ccagccggtc gcggaggcca tggatgcgat tgctgcggcc gatcttagcc agacgagcgg
     3661 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     3721 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     3781 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     3841 gcacctcgtg catgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     3901 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     3961 cctcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     4021 gcatccggag cttgcaggat cgccgcgcct ccgggcgtat atgctccgca ttggtcttga
     4081 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     4141 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     4201 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     4261 ccccagcact cgtccgaggg caaaggaata gagtagatgc cgaccgatcc ccactaccgc
     4321 attaagacct cagcgcggcc gcaaatttaa ataaaatgaa gtgaagttcc tatactttct
     4381 agagaatagg aacttctata gtgagtcgaa taagggcgac acaaaattta ttctaaatgc
     4441 ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc gttgacatgt
     4501 ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta ttttcttaat
     4561 tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata gatgaatagt
     4621 ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt gcgttgcttt
     4681 tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag gcgcccttaa
     4741 atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc cgaagcgggt
     4801 ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg gggcccgagc
     4861 ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa aaaggccatc
     4921 cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc cttccgcttc
     4981 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5041 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg ctaactacgg
     5581 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5701 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5761 tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg gattttggtc
     5821 atgagcttgc gccgtcccgt caagtcagcg taatgctctg cttaccaatg cttaatcagt
     5881 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5941 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc gatgataccg
     6001 cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc cggaagggcc
     6061 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6121 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc catcgctaca
     6181 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6241 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6301 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcgcta
     6361 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6421 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6481 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6541 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6601 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6661 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6721 atattcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6781 tacatatttg aatgtattta gaaaaataaa caaatagggg tcagtgttac aaccaattaa
     6841 ccaattctga acattatcgc gagcccattt atacctgaat atggctcata acaccccttg
     6901 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
     6961 acgccgtagc gccgatggta gtgtggggac tccccatgcg agagtaggga actgccaggc
     7021 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgcccgggc taattatggg
     7081 gtgtcgccct tattcgactc tatagtgaag ttcctattct ctagaaagta taggaacttc
     7141 tgaagtgggg atttaaatgc ggccgcgctg agggtttaat cgacgaagca gctgacggcc
     7201 agtgccaagc ttaacgcgta ccgggcccag tatatgttcc gcagatgact ggagctctgc
     7261 catacgtgcc ctctcaagca ccatttgttc catctacaga gactagtcac caactagtct
     7321 atcaagactc acagggtaca ttgctgagac caactgacca gaggcagggt agcggattga
     7381 cggctccatc tccttcactt acaaggtcta ttgaaagccc tttagcatca ccaagcggag
     7441 aatagattgt taagcttatt ttttgtatac tgttttgtga tagcacgaag tttttccacg
     7501 gtatcttgtt aaaaatatat atttgtggcg ggcttaccta catcaaatta ataagagact
     7561 aattataaac taaacacaca agcaagctac tttagggtaa aagtttataa atgcttttga
     7621 cgtataaacg ttgcttgtat ttattattac aattaaaggt ggatagaaaa cctagagact
     7681 agttagaaac taatctcagg tttgcgttaa actaaatcag agcccgagag gttaacagaa
     7741 cctagaaggg gactagatat ccgggtaggg aaacaaaaaa aaaaacaaga cagccacata
     7801 ttagggagac tagttagaag ctagttccag gactaggaaa ataaaagaca atgataccac
     7861 agtctagttg acaactagat agattctaga ttgaggccaa agtctctgag atccaggtta
     7921 gttgcaacta atactagtta gtatctagtc tcctataact ctgaagctag aataacttac
     7981 tactattatc ctcaccactg ttcagctgcg caaacggagt gattgcaagg tgttcagaga
     8041 ctagttattg actagtcagt gactagcaat aactaacaag gtattaacct accatgtctg
     8101 ccatcaccct gcacttcctc gggctcagca gccttttcct cctcattttc atgctcattt
     8161 tccttgttta agactgtgac tagtcaaaga ctagtccaga accacaaagg agaaatgtct
     8221 taccactttc ttcattgctt gtctcttttg cattatccat gtctgcaact agttagagtc
     8281 tagttagtga ctagtccgac gaggacttgc ttgtctccgg attgttggag gaactctcca
     8341 gggcctcaag atccacaaca gagccttcta gaagactggt caataactag ttggtctttg
     8401 tctgagtctg acttacgagg ttgcatactc gctccctttg cctcgtcaat cgatgagaaa
     8461 aagcgccaaa actcgcaata tggctttgaa ccacacggtg ctgagactag ttagaatcta
     8521 gtcccaaact agcttggata gcttaccttt gccctttgcg ttgcgacagg tcttgcaggg
     8581 tatggttcct ttctcaccag ctgatttagc tgccttgcta ccctcacggc ggatctgcat
     8641 aaagagtggc tagaggttat aaattagcac tgatcctagg tacggggctg aatgtaactt
     8701 gccyttcctt tctcatcgcg cggcaagaca ggcttgctca aattcctacc agtcacaggg
     8761 gtatgcacgg cgtacggacc acttgaacta gtcacagatt agttagcaac tagtctgcat
     8821 tgaatggctg tacttacggg ccctcgccat tgtcctgatc atttccagct tcaccctcgt
     8881 tgctgcaaag tagttagtga ctagtcaagg actagttgaa atgggagaag aaactcacga
     8941 attctcgaca cccttagtat tgtggtcctt ggacttggtg ctgctatata ttagctaata
     9001 cactagttag actcacagaa acttacgcag ctcgcttgcg cttcttggta ggagtcgggg
     9061 ttgggagaac agtgccttca aacaagcctt cataccatgc tacttgacta gtcagggact
     9121 agtcaccaag taatctagat aggacttgcc tttggcctcc atcagttcct tcatagtggg
     9181 aggtccattg tgcaatgtaa actccatgcc gtgggagttc ttatccttca agtgcttgac
     9241 caatatgttt ctgttggcag agggaacctg tcaactagtt aataactagt cagaaactag
     9301 tatagcagta gactcactgt acgcttgagg catcccttca ctcggcagta gacttcatat
     9361 ggatggatat caggcacgcc attgtcgtcc tgtggactag tcagtaacta ggcttaaagc
     9421 tagtcgggtc ggcttactat cttgaaatcc ggcagcgtaa gctccccgtc cttaactgcc
     9481 tcgagatagt gacagtactc tggggacttt cggagatcgt tatcgcgaat gctcggcata
     9541 ctaatcgttg actagtcttg gactagtccc gagcaaaaag gattggagga ggaggaggaa
     9601 ggtgagagtg agacaaagag cgaaataaga gcttcaaagg ctatctctaa gcagtatgaa
     9661 ggttaagtat ctagttcttg actagattta aaagagattt cgactagtta tgtacctgga
     9721 gtttggatat aggaatgtgt tgtggtaacg aaatgtaagg gggaggaaag aaaaagtcgg
     9781 tcaagaggta actctaagtc ggccattcct ttttgggagg cgctaaccat aaacggcatg
     9841 gtcgacttag agttagctca gggaatttag ggagttatct gcgaccaccg aggaacggcg
     9901 gaatgccaaa gaatcccgat ggagctctag ctggcggttg acaaccccac cttttggcgt
     9961 ttctgcggcg ttgcaggcgg gactggatac ttcgtagaac cagaaaggca aggcagaacg
    10021 cgctcagcaa gagtgttgga agtgatagca tgatgtgcct tgttaactag gtcaaaatct
    10081 gcagtatgct tgatgttatc caaagtgtga gagaggaagg tccaaacata cacgattggg
    10141 agagggccta ggtataagag tttttgagta gaacgcatgt gagcccagcc atctcgagga
    10201 gattaaacac gggccggcat ttgatggcta tgttagtacc ccaatggaaa cggtgagagt
    10261 ccagtggtcg cagataactc cctaaattcc ctgagctaac tctaagtcga ccatgccgtt
    10321 tatggttagc gcctcccaaa aaggaatggc cgacttagag ttacctcttg accgactttt
    10381 tctttcctcc cccttacatt tcgttaccac aacacattcc tatatccaaa ctccaggtac
    10441 ataactagtc gaaatctctt ttaaatctag tcaagaacta gatacttaac cttcatactg
    10501 cttagagata gcctttgaag ctcttatttc gctctttgtc tcactctcac cttcctcctc
    10561 ctcctccaat cctttttgct cgggactagt ccaagactag tcaacgatta gtatgccgag
    10621 cattcgcgat aacgatctcc gaaagtcccc agagtactgt cactatctcg aggcagttaa
    10681 ggacggggag cttacgctgc cggatttcaa gatagtaagc cgacccgact agctttaagc
    10741 ctagttactg actagtccac aggacgacaa tggcgtgcct gatatccatc catatgaagt
    10801 ctactgccga gtgaagggat gcctcaagcg tacagtgagt ctactgctat actagtttct
    10861 gactagttat taactagttg acaggttccc tctgccaaca gaaacatatt ggtcaagcac
    10921 ttgaaggaca agaactccca cggcatggag tttacattgc acaatggacc tcccactatg
    10981 aaggaactga tggaggccaa aggcaagtcc tatctagatt acttggtgac tagtccctga
    11041 ctagtcaagt agcatggtat gaaggcttgt ttgaaggcac tgttctccca accccgactc
    11101 ctaccaagaa gcgcaagcga gctgcgtaag tttctgtgag tctaactagt gtattagcta
    11161 atatatagca gcaccaagtc caaggaccac aatactaagg gtgtcgagaa ttcgtgagtt
    11221 tcttctccca tttcaactag tccttgacta gtcactaact actttgcagc aacgagggtg
    11281 aagctggaaa tgatcaggac aatggcgagg gcccgtaagt acagccattc aatgcagact
    11341 agttgctaac taatctgtga ctagttcaag tggtccgtac gccgtgcata cccctgtgac
    11401 tggtaggaat ttgagcaagc ctgtcttgcc gcgcgatgag aaaggaargg caagttacat
    11461 tcagccccgt acctaggatc agtgctaatt tataacctct agccactctt tatgcagatc
    11521 cgccgtgagg gtagcaaggc agctaaatca gctggtgaga aaggaaccat accctgcaag
    11581 acctgtcgca acgcaaaggg caaaggtaag ctatccaagc tagtttggga ctagattcta
    11641 actagtctca gcaccgtgtg gttcaaagcc atattgcgag ttttggcgct ttttctcatc
    11701 gattgacgag gcaaagggag cgagtatgca acctcgtaag tcagactcag acaaagacca
    11761 actagttatt gaccagtctt ctagaaggct ctgttgtgga tcttgaggcc ctggagagtt
    11821 cctccaacaa tccggagaca agcaagtcct cgtcggacta gtcactaact agactctaac
    11881 tagttgcaga catggataat gcaaaagaga caagcaatga agaaagtggt aagacatttc
    11941 tcctttgtgg ttctggacta gtctttgact agtcacagtc ttaaacaagg aaaatgagca
    12001 tgaaaatgag gaggaaaagg ctgctgagcc cgaggaagtg cagggtgatg gcagacatgg
    12061 taggttaata ccttgttagt tattgctagt cactgactag tcaataacta gtctctgaac
    12121 accttgcaat cactccgttt gcgcagctga acagtggtga ggataatagt agtaagttat
    12181 tctagcttca gagttatagg agactagata ctaactagta ttagttgcaa ctaacctgga
    12241 tctcagagac tttggcctca atctagaatc tatctagttg tcaactagac tgtggtatca
    12301 ttgtctttta ttttcctagt cctggaacta gcttctaact agtctcccta atatgtggct
    12361 gtcttgtttt ttttttttgt ttccctaccc ggatatctag tccccttcta ggttctgtta
    12421 acctctcggg ctctgattta gtttaacgca aacctgagat tagtttctaa ctagtctcta
    12481 ggttttctat ccacctttaa ttgtaataat aaatacaagc aacgtttata cgtcaaaagc
    12541 atttataaac ttttacccta aagtagcttg cttgtgtgtt tagtttataa ttagtctctt
    12601 attaatttga tgtaggtaag cccgccacaa atatatattt ttaacaagat accgtggaaa
    12661 aacttcgtgc tatcacaaaa cagtatacaa aaaataagct
//
