LOCUS       sequence-483813-       12117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EGFP in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Guoliang Yuan, Tatiana Serrano-Zayas, Ziyu Dai, Erica I. DeMers, 
            Shuang Deng, Beth A. Hofstad,, Kyle R. Pomraning
  TITLE     Strong Translation Elongation Factor 1-Alpha (tef1-alpha) Promoter 
            Reporter Systems for Aspergillus niger
  JOURNAL   ACS Synthetic Biology Article ASAP
REFERENCE   2  (bases 1 to 12117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology Article ASAP"
COMMENT     Sequence Label: pGY199
FEATURES             Location/Qualifiers
     source          1..12117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1143..1145,1146..1859)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1143..1145,1146..1856)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1185..1206)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1446..1465)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1793..1814
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2682..3707
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(3779..3812)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4282..4298)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4397..4414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4568..5156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4648..4667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5278..6069,6070..6138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5901..5920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6139..6230)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6272..6291
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6297..6316
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    6523..6556
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      6869..12116
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 aacaatctat tctccgcttg gtgatgctaa agggctttca atagaccttg taagtgaagg
       61 agatggagcc gtcaatccgc taccctgcct ctggtcagtt ggtctcagca atgtaccctg
      121 tgagtcttga tagactagtt ggtgactagt ctctgtagat ggaacaaatg gtgcttgaga
      181 gggcacgtat ggcagagctc cagtcatctg cggaacatat actgggcccg ggaagatctc
      241 atggtcatag ctgtttccgc tgagggttta atcgttgacc tcacagggat ttccaccaaa
      301 aagtggaccg ggcgggcgcc actcggccgt gccacagcag cctgagagcg gacaaataac
      361 aacagccgcc tgccgcgggg ttcggttgca aacatgacca acaggccagg ccatcatcaa
      421 cccaccgctg cgttgatgcc caggatttca gtccaataat ccacaattta ccaacggata
      481 gagctaggtg aactagatag acaggagggc cagagggagg ggaccgagat gaaaaatttt
      541 cgatgaaaga gtggtcaagg tggggtcgta gttcggcgct ccgagggcga ggaaccaagg
      601 aaaggcgaga aaaggacagg ctgatcgcgc tgcgttgctg ggctgcaagc gtgtccagtt
      661 gagtctggaa aaggctccgc cgtgaagatt ctgcgttggt cccgcacctg cgcggtgggg
      721 gcattacccc tccatgtcca atgatttcaa gtcaaagcca agggttgaag cccgcccgct
      781 tagtcgcctt ctcgcttgac ccctccatat aagtatttcc cctcctcccc ctcccacaaa
      841 tttttccttt ccctttcctc cctcgtccgc ttcagtacgt atatcttccc cccctctctc
      901 ttccttctca ctcttctctc cttctttctt gattcatcct ctctctaact gacttctttg
      961 ctcagcacct ctacgcgttc tggccgtagt atctgagcaa tttttctaca gactttttct
     1021 atctaattcc aaaaaagaac ttcgagttca ttcacaaccg tcatcatggg gtgagtcaag
     1081 acaacgatca tcatctgaca aaggaaggac atgggctaac ggatatcttc tacaacagta
     1141 aggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1201 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1261 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1321 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1381 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1441 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1501 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1561 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     1621 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1681 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1741 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1801 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     1861 cggacattcg atttatgccg ttatgacttc cttaaaaaag cctttacgaa tgaaagaaat
     1921 ggaattagac ttgttatgta gttgattcta caatggatta tgattcctga acttcaaatc
     1981 cgctgttcat tattaatctc agctcttccc gtaaagccaa tgttgaaact attcgtaaat
     2041 gtacctcgtt ttgcgtgtac cttgcttatc acgtgatatt acatgacctg gacagagttc
     2101 tgcgcgaaag tcataacgta aatcccgggc ggtaggtgcg tcccgggcgg aaggtagttt
     2161 tctcgtccac cccaacgcgt ttatcaacct caactttcaa caaccatcat gccaccaaaa
     2221 gcgcgtaaaa caaagcgaga tttgattgag caagagggca ggatccaatg cgcgattcaa
     2281 gacattaaaa atggaaaatt tcaaaaaatt gcgcccgcag cgcgtgcata caaaattcat
     2341 cccaatacaa gtgtaatgct agtggaggtc aacacatcaa tgcctatttt ggtttagtcg
     2401 tccaggcggt gagcacaaaa tttgtgtcgt ttgacaagat ggttcattta ggcaactggt
     2461 cagatcagcc ccacttgtag cagtagcggc ggcgctcgaa gtgtgactct tattagcaga
     2521 caggaacgag gacattatta tcatctgctg cttggtgcac gataacttgg tgcgtttgtc
     2581 aagcaaggta agtggacgac ccggtcatac cttcttaagt tcgcccttcc tccctttatt
     2641 tcagattcaa tctgacttac ctattctacc caagcatcca aatgaaaaag cctgaactca
     2701 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     2761 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     2821 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     2881 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggagttc agcgagagcc
     2941 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     3001 aactgcccgc tgttctccag ccggtcgcgg aggccatgga tgcgattgct gcggccgatc
     3061 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     3121 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     3181 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     3241 actgccccga agtccggcac ctcgtgcatg cggatttcgg ctccaacaat gtcctgacgg
     3301 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     3361 acgaggtcgc caacatcctc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     3421 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcgcctccgg gcgtatatgc
     3481 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     3541 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     3601 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     3661 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt agatgccgac
     3721 cgatccccac taccgcatta agacctcagc gcggccgcaa atttaaataa aatgaagtga
     3781 agttcctata ctttctagag aataggaact tctatagtga gtcgaataag ggcgacacaa
     3841 aatttattct aaatgcataa taaatactga taacatctta tagtttgtat tatattttgt
     3901 attatcgttg acatgtataa ttttgatatc aaaaactgat tttcccttta ttattttcga
     3961 gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa aaaatcataa
     4021 ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac gtatcttatt
     4081 taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata tcaagcaaag
     4141 tgacaggcgc ccttaaatat tctgacaaat gctctttccc taaactcccc ccataaaaaa
     4201 acccgccgaa gcgggttttt acgttatttg cggattaacg attactcgtt atcagaaccg
     4261 cccagggggc ccgagcttaa gactggccgt cgttttacaa cacagaaaga gtttgtagaa
     4321 acgcaaaaag gccatccgtc aggggccttc tgcttagttt gatgcctggc agttccctac
     4381 tctcgccttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4441 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4501 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4741 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4981 ggtgggctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgacgcgcgc gtaactcacg
     5221 ttaagggatt ttggtcatga gcttgcgccg tcccgtcaag tcagcgtaat gctctgctta
     5281 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5341 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5401 cgctgcgatg ataccgcgag aaccacgctc accggctccg gatttatcag caataaacca
     5461 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5521 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5581 tgttgccatc gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5641 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5701 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5761 ggttatggca gcgctacata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5821 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5881 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5941 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6001 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6061 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6121 gaaatgttga atactcatat tcttcctttt tcaatattat tgaagcattt atcagggtta
     6181 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggtcag
     6241 tgttacaacc aattaaccaa ttctgaacat tatcgcgagc ccatttatac ctgaatatgg
     6301 ctcataacac cccttgtttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
     6361 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggactccc catgcgagag
     6421 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgc
     6481 ccgggctaat tatggggtgt cgcccttatt cgactctata gtgaagttcc tattctctag
     6541 aaagtatagg aacttctgaa gtggggattt aaatgcggcc gcgctgaggg tttaatcgac
     6601 gaagcagctg acggccagtg ccaagcttaa cgcgtaccgg gcccagtata tgttccgcag
     6661 atgactggag ctctgccata cgtgccctct caagcaccat ttgttccatc tacagagact
     6721 agtcaccaac tagtctatca agactcacag ggtacattgc tgagaccaac tgaccagagg
     6781 cagggtagcg gattgacggc tccatctcct tcacttacaa ggtctattga aagcccttta
     6841 gcatcaccaa gcggagaata gattgttaag cttatttttt gtatactgtt ttgtgatagc
     6901 acgaagtttt tccacggtat cttgttaaaa atatatattt gtggcgggct tacctacatc
     6961 aaattaataa gagactaatt ataaactaaa cacacaagca agctacttta gggtaaaagt
     7021 ttataaatgc ttttgacgta taaacgttgc ttgtatttat tattacaatt aaaggtggat
     7081 agaaaaccta gagactagtt agaaactaat ctcaggtttg cgttaaacta aatcagagcc
     7141 cgagaggtta acagaaccta gaaggggact agatatccgg gtagggaaac aaaaaaaaaa
     7201 acaagacagc cacatattag ggagactagt tagaagctag ttccaggact aggaaaataa
     7261 aagacaatga taccacagtc tagttgacaa ctagatagat tctagattga ggccaaagtc
     7321 tctgagatcc aggttagttg caactaatac tagttagtat ctagtctcct ataactctga
     7381 agctagaata acttactact attatcctca ccactgttca gctgcgcaaa cggagtgatt
     7441 gcaaggtgtt cagagactag ttattgacta gtcagtgact agcaataact aacaaggtat
     7501 taacctacca tgtctgccat caccctgcac ttcctcgggc tcagcagcct tttcctcctc
     7561 attttcatgc tcattttcct tgtttaagac tgtgactagt caaagactag tccagaacca
     7621 caaaggagaa atgtcttacc actttcttca ttgcttgtct cttttgcatt atccatgtct
     7681 gcaactagtt agagtctagt tagtgactag tccgacgagg acttgcttgt ctccggattg
     7741 ttggaggaac tctccagggc ctcaagatcc acaacagagc cttctagaag actggtcaat
     7801 aactagttgg tctttgtctg agtctgactt acgaggttgc atactcgctc cctttgcctc
     7861 gtcaatcgat gagaaaaagc gccaaaactc gcaatatggc tttgaaccac acggtgctga
     7921 gactagttag aatctagtcc caaactagct tggatagctt acctttgccc tttgcgttgc
     7981 gacaggtctt gcagggtatg gttcctttct caccagctga tttagctgcc ttgctaccct
     8041 cacggcggat ctgcataaag agtggctaga ggttataaat tagcactgat cctaggtacg
     8101 gggctgaatg taacttgccy ttcctttctc atcgcgcggc aagacaggct tgctcaaatt
     8161 cctaccagtc acaggggtat gcacggcgta cggaccactt gaactagtca cagattagtt
     8221 agcaactagt ctgcattgaa tggctgtact tacgggccct cgccattgtc ctgatcattt
     8281 ccagcttcac cctcgttgct gcaaagtagt tagtgactag tcaaggacta gttgaaatgg
     8341 gagaagaaac tcacgaattc tcgacaccct tagtattgtg gtccttggac ttggtgctgc
     8401 tatatattag ctaatacact agttagactc acagaaactt acgcagctcg cttgcgcttc
     8461 ttggtaggag tcggggttgg gagaacagtg ccttcaaaca agccttcata ccatgctact
     8521 tgactagtca gggactagtc accaagtaat ctagatagga cttgcctttg gcctccatca
     8581 gttccttcat agtgggaggt ccattgtgca atgtaaactc catgccgtgg gagttcttat
     8641 ccttcaagtg cttgaccaat atgtttctgt tggcagaggg aacctgtcaa ctagttaata
     8701 actagtcaga aactagtata gcagtagact cactgtacgc ttgaggcatc ccttcactcg
     8761 gcagtagact tcatatggat ggatatcagg cacgccattg tcgtcctgtg gactagtcag
     8821 taactaggct taaagctagt cgggtcggct tactatcttg aaatccggca gcgtaagctc
     8881 cccgtcctta actgcctcga gatagtgaca gtactctggg gactttcgga gatcgttatc
     8941 gcgaatgctc ggcatactaa tcgttgacta gtcttggact agtcccgagc aaaaaggatt
     9001 ggaggaggag gaggaaggtg agagtgagac aaagagcgaa ataagagctt caaaggctat
     9061 ctctaagcag tatgaaggtt aagtatctag ttcttgacta gatttaaaag agatttcgac
     9121 tagttatgta cctggagttt ggatatagga atgtgttgtg gtaacgaaat gtaaggggga
     9181 ggaaagaaaa agtcggtcaa gaggtaactc taagtcggcc attccttttt gggaggcgct
     9241 aaccataaac ggcatggtcg acttagagtt agctcaggga atttagggag ttatctgcga
     9301 ccaccgagga acggcggaat gccaaagaat cccgatggag ctctagctgg cggttgacaa
     9361 ccccaccttt tggcgtttct gcggcgttgc aggcgggact ggatacttcg tagaaccaga
     9421 aaggcaaggc agaacgcgct cagcaagagt gttggaagtg atagcatgat gtgccttgtt
     9481 aactaggtca aaatctgcag tatgcttgat gttatccaaa gtgtgagaga ggaaggtcca
     9541 aacatacacg attgggagag ggcctaggta taagagtttt tgagtagaac gcatgtgagc
     9601 ccagccatct cgaggagatt aaacacgggc cggcatttga tggctatgtt agtaccccaa
     9661 tggaaacggt gagagtccag tggtcgcaga taactcccta aattccctga gctaactcta
     9721 agtcgaccat gccgtttatg gttagcgcct cccaaaaagg aatggccgac ttagagttac
     9781 ctcttgaccg actttttctt tcctccccct tacatttcgt taccacaaca cattcctata
     9841 tccaaactcc aggtacataa ctagtcgaaa tctcttttaa atctagtcaa gaactagata
     9901 cttaaccttc atactgctta gagatagcct ttgaagctct tatttcgctc tttgtctcac
     9961 tctcaccttc ctcctcctcc tccaatcctt tttgctcggg actagtccaa gactagtcaa
    10021 cgattagtat gccgagcatt cgcgataacg atctccgaaa gtccccagag tactgtcact
    10081 atctcgaggc agttaaggac ggggagctta cgctgccgga tttcaagata gtaagccgac
    10141 ccgactagct ttaagcctag ttactgacta gtccacagga cgacaatggc gtgcctgata
    10201 tccatccata tgaagtctac tgccgagtga agggatgcct caagcgtaca gtgagtctac
    10261 tgctatacta gtttctgact agttattaac tagttgacag gttccctctg ccaacagaaa
    10321 catattggtc aagcacttga aggacaagaa ctcccacggc atggagttta cattgcacaa
    10381 tggacctccc actatgaagg aactgatgga ggccaaaggc aagtcctatc tagattactt
    10441 ggtgactagt ccctgactag tcaagtagca tggtatgaag gcttgtttga aggcactgtt
    10501 ctcccaaccc cgactcctac caagaagcgc aagcgagctg cgtaagtttc tgtgagtcta
    10561 actagtgtat tagctaatat atagcagcac caagtccaag gaccacaata ctaagggtgt
    10621 cgagaattcg tgagtttctt ctcccatttc aactagtcct tgactagtca ctaactactt
    10681 tgcagcaacg agggtgaagc tggaaatgat caggacaatg gcgagggccc gtaagtacag
    10741 ccattcaatg cagactagtt gctaactaat ctgtgactag ttcaagtggt ccgtacgccg
    10801 tgcatacccc tgtgactggt aggaatttga gcaagcctgt cttgccgcgc gatgagaaag
    10861 gaarggcaag ttacattcag ccccgtacct aggatcagtg ctaatttata acctctagcc
    10921 actctttatg cagatccgcc gtgagggtag caaggcagct aaatcagctg gtgagaaagg
    10981 aaccataccc tgcaagacct gtcgcaacgc aaagggcaaa ggtaagctat ccaagctagt
    11041 ttgggactag attctaacta gtctcagcac cgtgtggttc aaagccatat tgcgagtttt
    11101 ggcgcttttt ctcatcgatt gacgaggcaa agggagcgag tatgcaacct cgtaagtcag
    11161 actcagacaa agaccaacta gttattgacc agtcttctag aaggctctgt tgtggatctt
    11221 gaggccctgg agagttcctc caacaatccg gagacaagca agtcctcgtc ggactagtca
    11281 ctaactagac tctaactagt tgcagacatg gataatgcaa aagagacaag caatgaagaa
    11341 agtggtaaga catttctcct ttgtggttct ggactagtct ttgactagtc acagtcttaa
    11401 acaaggaaaa tgagcatgaa aatgaggagg aaaaggctgc tgagcccgag gaagtgcagg
    11461 gtgatggcag acatggtagg ttaatacctt gttagttatt gctagtcact gactagtcaa
    11521 taactagtct ctgaacacct tgcaatcact ccgtttgcgc agctgaacag tggtgaggat
    11581 aatagtagta agttattcta gcttcagagt tataggagac tagatactaa ctagtattag
    11641 ttgcaactaa cctggatctc agagactttg gcctcaatct agaatctatc tagttgtcaa
    11701 ctagactgtg gtatcattgt cttttatttt cctagtcctg gaactagctt ctaactagtc
    11761 tccctaatat gtggctgtct tgtttttttt ttttgtttcc ctacccggat atctagtccc
    11821 cttctaggtt ctgttaacct ctcgggctct gatttagttt aacgcaaacc tgagattagt
    11881 ttctaactag tctctaggtt ttctatccac ctttaattgt aataataaat acaagcaacg
    11941 tttatacgtc aaaagcattt ataaactttt accctaaagt agcttgcttg tgtgtttagt
    12001 ttataattag tctcttatta atttgatgta ggtaagcccg ccacaaatat atatttttaa
    12061 caagataccg tggaaaaact tcgtgctatc acaaaacagt atacaaaaaa taagctt
//
