LOCUS       sequence-483815-        9734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sleeping Beauty XPRESSO vector for optogenetic CoChR-eGFP 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Wexler Y, Grinstein H, Huber I, Glatstein S, Ghiringhelli M, Edri O,
            Landesberg M, Shiff D, Arbel G, Rosh I, Choudhary A, Stern S, 
            Gepstein L
  TITLE     XPRESSO: Rapid genetic engineering of human pluripotent stem cells 
            for durable overexpression using a modular anti-silencing vector.
  JOURNAL   Stem Cell Reports. 2025 Aug 19:102603. doi: 
            10.1016/j.stemcr.2025.102603.
  PUBMED    40845851
REFERENCE   2  (bases 1 to 9734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2025 Aug 19:102603. doi: 10.1016/j.stemcr.2025.102603."
COMMENT     Sequence Label: pSB-XPRESSO-CoChR-EGFP
FEATURES             Location/Qualifiers
     source          1..9734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    324..548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     537..554
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    587..1175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             693..704
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1102..1122
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1206..1919,1920..1922))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(1209..1919,1920..1922))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1251..1272)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1600..1619
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(1941..2804)
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFSIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVILIHLSNLTGLKDDYSKRTMRLLVSDVGTIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSKNCWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     primer_bind     complement(2880..2899)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          2907..3923
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(2961..2983)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(3924..4200)
                     /label=chicken beta-actin promoter
     enhancer        4202..4581
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             5752..6453
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5885..5903)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6153..6172
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6347..6370
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             6463..6519
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6520..7119
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6520..6539)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7016..7036
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7219..7238)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7284..7339
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7338..7357)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7731..7748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7902..8490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7982..8001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8661..9452,9453..9521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9284..9303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9522..9626)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agtaggccca
      301 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt
      361 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
      421 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
      481 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
      541 aggcacagtc gaggctgatc agcgagctca ggtcgacggt atcgatgcgg ggaggcggcc
      601 caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc
      661 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
      721 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
      781 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
      841 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
      901 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
      961 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     1021 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     1081 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     1141 aatctttcac aaattttgta atccagaggt tgattatcga aagcttggcc tgacaggccg
     1201 tctcattact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     1261 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
     1321 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     1381 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
     1441 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
     1501 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     1561 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     1621 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     1681 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     1741 acgagggtgg gccagggcac gggcagcttg ccggtggtgc aaatgaactt cagggtcagc
     1801 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     1861 tcgccgtcta gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     1921 actgctacta ccggtgccgc cactgtctcc tcgtcctcct ggtccaccat ggtctccact
     1981 tccatctcct cgccggccac gttgatcttg gtcttcttcc ggatgtcgcc gtagatgatg
     2041 atgtgctggt ggatcagcac tctcagatag tgtcccagca ggccccaaca attcttgctc
     2101 atgaggtcga tgatggtgtg gccaatggta gagccgtaca cgctgatggc gtcgaatcct
     2161 tctggtccca cgacgaacag cacggggaac atgccccaag acaggaagaa cagccaagcc
     2221 atgatccgca cgacggttct aggtctgccc tttggcacca cgtggtagct ctcgatatac
     2281 accttggcgg cgtggaagaa ggtgttggcg ccgtagatgc aacccagcac gaagaagatg
     2341 accttgacgt agcctgtgct catggcgctt gtagctcccc acacgatggt tcccacgtct
     2401 gacaccagca gcctcatggt ccgcttgctg tagtcgtcct tcaggccggt caggttgctc
     2461 aggtggatca ggatgacggg acaagtcagc agccactctg cgtatctcag ccactggact
     2521 cggtgtccgt tagccaggta cagcatgctg gggtcgtcga actcgtggaa gaactcgatg
     2581 atgaccttgg tcagctcgac gcaacagacg tagacctcct cccaaccgca agtggctctc
     2641 caagtctggt aggcgtagaa catcaggatc aggatgctga agccgaaggc gaaccactgg
     2701 aggatgttgg ccaccagctg ctctgtatcg cttcccaggc tgtcggtcca agccaggcaa
     2761 aagcactggt cgataggcac aatggcgctg ccgtttccca gcatggtgcg gtggcggcct
     2821 cagaggccaa cagcggccgc gatatcctcg aggaattctt tgccaaaatg atgagacagc
     2881 acaataacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg aacatggtta
     2941 gcagaggctc tagagccgcc ggtcacacgc cagaagccga accccgccct gccccgtccc
     3001 ccccgaaggc agccgtcccc ccgcggacag ccccgaggct ggagagggag aaggggacgg
     3061 cggcgcggcg acgcacgaag gccctccccg cccatttcct tcctgccggc gccgcaccgc
     3121 ttcgccccgc gcccgctaga gggggtgcgg cggcgcctcc cagatttcgg ctccgcacag
     3181 atttgggaca aaggaagtcc ctgcgccctc tcgcacgatt accataaaag gcaatggctg
     3241 cggctcgccg cgcctcgaca gccgccggcg ctccgggggc cgccgcgccc ctcccccgag
     3301 cccyccccgg cccgaggcgg ccccgccccg cccggcaccc ccacctgccg ccaccccccg
     3361 cccggcacgg cgagccccgc gccacgcccc gtacggagcc ccgcacccga agccgggccg
     3421 tgctcagcaa ctcggggagg ggggtgcagg gggggttgca gcccgaccga cgcgcccaca
     3481 ccccctgctc acccccccac gcacacaccc cgcacgcagc ctttgttccc ctcgcagccc
     3541 cccccgcacc gcggggcacc gcccccggcc gcgctcccct cgcgcacact gcggagcgca
     3601 caaagccccg cgccgcgccc gcagcgctca cagccgccgg gcagcgcgga gccgcacgcg
     3661 gcgctcccca cgcacacaca cacgcacgca ccccccgagc cgctcccccc gcacaaaggg
     3721 ccctcccgga gcccctcaag gctttcacgc agccacagaa aagaaacaag ccgtcattaa
     3781 accaagcgct aattacagcc cggaggagaa gggccgtccc gcccgctcac ctgtgggagt
     3841 aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg cggcggagcg gggcacgggg
     3901 cgaaggcagc gcgcagcgac tcccgcccgc cgcgcgcttc gctttttata gggccgccgc
     3961 cgccgccgcc tcgccataaa aggaaacttt cggagcgcgc cgctctgatt ggctgccgcc
     4021 scacctctcc scctcscccc sccccccccc tccccccccc ccsccccccc tgggcgcgcc
     4081 cccccccccc ccccgccccc atcgctgcac aaaataatta aaaaataaat aaatacaaaa
     4141 ttgggggtgg ggaggggggg gagatgggga gagtgaagca gaacgtgggg ctcacctcga
     4201 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     4261 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     4321 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     4381 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     4441 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     4501 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     4561 aactagtcaa taatcaatgt cgactcgtat tcccgggctg ccaagcagaa aagccaccgc
     4621 tgaggagact ccggtcactg tcctcgcccc gcctccccct tccctcccct tggggaccac
     4681 cgggcgccac gccgcgaacg gtaagtgccg cggtcgtcgg cgcctccgcc ctccccctag
     4741 ggccccaatt cccagcgggc gcggcgccgg cccctccccc cgccgcgcgc gcgcccgctg
     4801 ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc ctccccccgg cggccccgcg
     4861 cgcagctccc ggctccctcc cccttcggat gtggcttgag ctgtaggcgc ggagggccgg
     4921 agacgctgca gacccgcgac ccggagcagc tcggaggcgg tgaagtcggt ggctttcctt
     4981 ctctctagct ctcgctcgct ggtggtgctt cagatgccac acgcgtagct tataataccg
     5041 gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc
     5101 cactgacggg caccggagcc tctaggcgag accctgtctc acaaaataaa gtaagcccgg
     5161 actgagtgcg gaaaggcggg cctggcgggt ctggtctccc catgcgggcc accagaggcc
     5221 ctgcagcctt cagtcgcttg aaggggtaat ggcgcttcca ctcacaaaca tggcggacag
     5281 agcgtgtgaa cgagatgaac agcccctcaa aaatatggcc gccgaggctg gacggccgtg
     5341 ccccagcagc accgcctccg cgccccacgt gatctctcgc cgggcacagc gctgaccgcg
     5401 gaggtccaac cggaagaatg tccggattgg acattcggaa gagggcccgc cttccctggg
     5461 gaatctctgc gcacgcgcag aacgcttcga ccaatgaaaa cacaggaagc cgtccgcgca
     5521 accgcgttgc gtcacttctg ccgcccctgt ttcaaggtat atagccgtag acggaacttc
     5581 gcctttctct cggccttagc gccatttttt tgggtgagtg ttttttggtt cctgcgttgg
     5641 gattccgtgt acaatccata gacatctgac ctcggcactt agcatcatca cagcaaacta
     5701 actgtagcct ttctctcttt ccctgtagaa acctctgcac ctgaggccac catgttgagc
     5761 aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     5821 aacggccatg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     5881 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     5941 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     6001 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     6061 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     6121 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gacaatgggc
     6181 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     6241 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     6301 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     6361 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     6421 ctgttcctgt acggcatgga cgagctgtac aagggcagtg gagctactaa cttcagcctg
     6481 ctgaagcagg ctggtgacgt cgaggagaat cctggcccca tgaccgagta caagcccacg
     6541 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6601 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6661 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6721 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6781 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6841 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6901 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6961 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     7021 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     7081 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat tcgaaggcct gtcgtgaatt
     7141 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     7201 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     7261 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     7321 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     7381 gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc catgaactag
     7441 ctactcggga ccccttaccg aaacatcgcc gcattctgca gaggagtcga gtgtatgtaa
     7501 acttctgacc cactgggaat gtgatgaaag aaataaaagc tgaaatgaat cattctctct
     7561 actattattc tgatatttca cattcttaaa ataaagtggt gatcctaact gacctaagac
     7621 agggaatttt tactaggatt aaatgtcagg aattgtgaaa aagtgagttt aaatgtattt
     7681 ggctaaggtg tatgtaaact tccgacttca actgtatagg gatccgcttc ctcgctcact
     7741 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7801 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7861 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7921 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7981 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8041 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8101 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8161 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8221 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8281 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8341 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8401 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8461 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8641 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8701 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8761 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8821 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8881 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8941 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9001 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9061 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9121 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9181 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9241 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9301 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9361 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9421 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9481 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9541 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9601 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg
     9661 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
     9721 attttgttaa aatt
//
