LOCUS       sequence-483823-       12654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express H2B-mRFP1 in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Guoliang Yuan, Tatiana Serrano-Zayas, Ziyu Dai, Erica I. DeMers, 
            Shuang Deng, Beth A. Hofstad,, Kyle R. Pomraning
  TITLE     Strong Translation Elongation Factor 1-Alpha (tef1-alpha) Promoter 
            Reporter Systems for Aspergillus niger
  JOURNAL   ACS Synthetic Biology Article ASAP
REFERENCE   2  (bases 1 to 12654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology Article ASAP"
COMMENT     Sequence Label: pGY224
FEATURES             Location/Qualifiers
     source          1..12654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1723..2397
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIKGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(1850..1868)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2118..2137
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2312..2335
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3220..4245
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4317..4350)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4820..4836)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4935..4952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5106..5694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5186..5205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5816..6607,6608..6676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6439..6458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6677..6768)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6810..6829
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6835..6854
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    7061..7094
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      7407..12654
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aagcctccca aagccgctga gaagaagccc agcaccggtg gcaaggcccc ggcaggcggc
     1201 aaggctcccg ctgagaagaa ggaagccggc aagaagactg ctgccgctgc ctccggcgac
     1261 aagaagaagc gtggaaagac caggaaggag acctactcgt cttacatcta caagggtgag
     1321 taatcttaag acttgtattc ctagtcaata gttgttgact gtgttatagt cttgaagcag
     1381 gtccaccctg ataccggtat ctcgactcgt gctatgtcca tcctgaactc ctttgtcaat
     1441 ggtatgtcgt gaatgaagca gggttatacc ttcagcaagc acctaacgcg gtctagatat
     1501 cttcgagcgt gtcgcgaccg aggcctcgaa acttgccgct tacaacaaga agtccaccat
     1561 ctcttcacgg gagatccaga cctcgtaagt cctctgtcga tcaagatagt agcaattcgc
     1621 taacacctct ctagtgtgag gctcatcctt cccggtgaat tggcgaagca cgcggtgtcg
     1681 gaaggcacca aggctgtcac gaagtactcg tcgtctgcca aagcctcctc cgaggacgtc
     1741 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
     1801 gagatcaagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1861 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca gttccagtac
     1921 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1981 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     2041 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     2101 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     2161 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg
     2221 aaggacggcg gccactacga cgccgaggtc aagaccacct acatggccaa gaagcccgtg
     2281 cagctgcccg gcgcctacaa gaccgacatc aagctggaca tcacctccca caacgaggac
     2341 tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg cgcctaagcg
     2401 gacattcgat ttatgccgtt atgacttcct taaaaaagcc tttacgaatg aaagaaatgg
     2461 aattagactt gttatgtagt tgattctaca atggattatg attcctgaac ttcaaatccg
     2521 ctgttcatta ttaatctcag ctcttcccgt aaagccaatg ttgaaactat tcgtaaatgt
     2581 acctcgtttt gcgtgtacct tgcttatcac gtgatattac atgacctgga cagagttctg
     2641 cgcgaaagtc ataacgtaaa tcccgggcgg taggtgcgtc ccgggcggaa ggtagttttc
     2701 tcgtccaccc caacgcgttt atcaacctca actttcaaca accatcatgc caccaaaagc
     2761 gcgtaaaaca aagcgagatt tgattgagca agagggcagg atccaatgcg cgattcaaga
     2821 cattaaaaat ggaaaatttc aaaaaattgc gcccgcagcg cgtgcataca aaattcatcc
     2881 caatacaagt gtaatgctag tggaggtcaa cacatcaatg cctattttgg tttagtcgtc
     2941 caggcggtga gcacaaaatt tgtgtcgttt gacaagatgg ttcatttagg caactggtca
     3001 gatcagcccc acttgtagca gtagcggcgg cgctcgaagt gtgactctta ttagcagaca
     3061 ggaacgagga cattattatc atctgctgct tggtgcacga taacttggtg cgtttgtcaa
     3121 gcaaggtaag tggacgaccc ggtcatacct tcttaagttc gcccttcctc cctttatttc
     3181 agattcaatc tgacttacct attctaccca agcatccaaa tgaaaaagcc tgaactcacc
     3241 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     3301 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     3361 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     3421 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggagttcag cgagagcctg
     3481 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     3541 ctgcccgctg ttctccagcc ggtcgcggag gccatggatg cgattgctgc ggccgatctt
     3601 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     3661 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     3721 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     3781 tgccccgaag tccggcacct cgtgcatgcg gatttcggct ccaacaatgt cctgacggac
     3841 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     3901 gaggtcgcca acatcctctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     3961 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc gcctccgggc gtatatgctc
     4021 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     4081 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     4141 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     4201 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg
     4261 atccccacta ccgcattaag acctcagcgc ggccgcaaat ttaaataaaa tgaagtgaag
     4321 ttcctatact ttctagagaa taggaacttc tatagtgagt cgaataaggg cgacacaaaa
     4381 tttattctaa atgcataata aatactgata acatcttata gtttgtatta tattttgtat
     4441 tatcgttgac atgtataatt ttgatatcaa aaactgattt tccctttatt attttcgaga
     4501 tttattttct taattctctt taacaaacta gaaatattgt atatacaaaa aatcataaat
     4561 aatagatgaa tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt atcttattta
     4621 aagtgcgttg cttttttctc atttataagg ttaaataatt ctcatatatc aagcaaagtg
     4681 acaggcgccc ttaaatattc tgacaaatgc tctttcccta aactcccccc ataaaaaaac
     4741 ccgccgaagc gggtttttac gttatttgcg gattaacgat tactcgttat cagaaccgcc
     4801 cagggggccc gagcttaaga ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac
     4861 gcaaaaaggc catccgtcag gggccttctg cttagtttga tgcctggcag ttccctactc
     4921 tcgccttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4981 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5041 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5101 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5161 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5221 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5281 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5341 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5401 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5461 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5521 tgggctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5581 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5641 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5701 cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgcgcgt aactcacgtt
     5761 aagggatttt ggtcatgagc ttgcgccgtc ccgtcaagtc agcgtaatgc tctgcttacc
     5821 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5881 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg
     5941 ctgcgatgat accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc
     6001 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6061 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6121 ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6181 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6241 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6301 ttatggcagc gctacataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6361 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6421 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6481 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6541 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6601 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6661 aatgttgaat actcatattc ttcctttttc aatattattg aagcatttat cagggttatt
     6721 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggtcagtg
     6781 ttacaaccaa ttaaccaatt ctgaacatta tcgcgagccc atttatacct gaatatggct
     6841 cataacaccc cttgtttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     6901 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggactcccca tgcgagagta
     6961 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgccc
     7021 gggctaatta tggggtgtcg cccttattcg actctatagt gaagttccta ttctctagaa
     7081 agtataggaa cttctgaagt ggggatttaa atgcggccgc gctgagggtt taatcgacga
     7141 agcagctgac ggccagtgcc aagcttaacg cgtaccgggc ccagtatatg ttccgcagat
     7201 gactggagct ctgccatacg tgccctctca agcaccattt gttccatcta cagagactag
     7261 tcaccaacta gtctatcaag actcacaggg tacattgctg agaccaactg accagaggca
     7321 gggtagcgga ttgacggctc catctccttc acttacaagg tctattgaaa gccctttagc
     7381 atcaccaagc ggagaataga ttgttaagct tattttttgt atactgtttt gtgatagcac
     7441 gaagtttttc cacggtatct tgttaaaaat atatatttgt ggcgggctta cctacatcaa
     7501 attaataaga gactaattat aaactaaaca cacaagcaag ctactttagg gtaaaagttt
     7561 ataaatgctt ttgacgtata aacgttgctt gtatttatta ttacaattaa aggtggatag
     7621 aaaacctaga gactagttag aaactaatct caggtttgcg ttaaactaaa tcagagcccg
     7681 agaggttaac agaacctaga aggggactag atatccgggt agggaaacaa aaaaaaaaac
     7741 aagacagcca catattaggg agactagtta gaagctagtt ccaggactag gaaaataaaa
     7801 gacaatgata ccacagtcta gttgacaact agatagattc tagattgagg ccaaagtctc
     7861 tgagatccag gttagttgca actaatacta gttagtatct agtctcctat aactctgaag
     7921 ctagaataac ttactactat tatcctcacc actgttcagc tgcgcaaacg gagtgattgc
     7981 aaggtgttca gagactagtt attgactagt cagtgactag caataactaa caaggtatta
     8041 acctaccatg tctgccatca ccctgcactt cctcgggctc agcagccttt tcctcctcat
     8101 tttcatgctc attttccttg tttaagactg tgactagtca aagactagtc cagaaccaca
     8161 aaggagaaat gtcttaccac tttcttcatt gcttgtctct tttgcattat ccatgtctgc
     8221 aactagttag agtctagtta gtgactagtc cgacgaggac ttgcttgtct ccggattgtt
     8281 ggaggaactc tccagggcct caagatccac aacagagcct tctagaagac tggtcaataa
     8341 ctagttggtc tttgtctgag tctgacttac gaggttgcat actcgctccc tttgcctcgt
     8401 caatcgatga gaaaaagcgc caaaactcgc aatatggctt tgaaccacac ggtgctgaga
     8461 ctagttagaa tctagtccca aactagcttg gatagcttac ctttgccctt tgcgttgcga
     8521 caggtcttgc agggtatggt tcctttctca ccagctgatt tagctgcctt gctaccctca
     8581 cggcggatct gcataaagag tggctagagg ttataaatta gcactgatcc taggtacggg
     8641 gctgaatgta acttgccytt cctttctcat cgcgcggcaa gacaggcttg ctcaaattcc
     8701 taccagtcac aggggtatgc acggcgtacg gaccacttga actagtcaca gattagttag
     8761 caactagtct gcattgaatg gctgtactta cgggccctcg ccattgtcct gatcatttcc
     8821 agcttcaccc tcgttgctgc aaagtagtta gtgactagtc aaggactagt tgaaatggga
     8881 gaagaaactc acgaattctc gacaccctta gtattgtggt ccttggactt ggtgctgcta
     8941 tatattagct aatacactag ttagactcac agaaacttac gcagctcgct tgcgcttctt
     9001 ggtaggagtc ggggttggga gaacagtgcc ttcaaacaag ccttcatacc atgctacttg
     9061 actagtcagg gactagtcac caagtaatct agataggact tgcctttggc ctccatcagt
     9121 tccttcatag tgggaggtcc attgtgcaat gtaaactcca tgccgtggga gttcttatcc
     9181 ttcaagtgct tgaccaatat gtttctgttg gcagagggaa cctgtcaact agttaataac
     9241 tagtcagaaa ctagtatagc agtagactca ctgtacgctt gaggcatccc ttcactcggc
     9301 agtagacttc atatggatgg atatcaggca cgccattgtc gtcctgtgga ctagtcagta
     9361 actaggctta aagctagtcg ggtcggctta ctatcttgaa atccggcagc gtaagctccc
     9421 cgtccttaac tgcctcgaga tagtgacagt actctgggga ctttcggaga tcgttatcgc
     9481 gaatgctcgg catactaatc gttgactagt cttggactag tcccgagcaa aaaggattgg
     9541 aggaggagga ggaaggtgag agtgagacaa agagcgaaat aagagcttca aaggctatct
     9601 ctaagcagta tgaaggttaa gtatctagtt cttgactaga tttaaaagag atttcgacta
     9661 gttatgtacc tggagtttgg atataggaat gtgttgtggt aacgaaatgt aagggggagg
     9721 aaagaaaaag tcggtcaaga ggtaactcta agtcggccat tcctttttgg gaggcgctaa
     9781 ccataaacgg catggtcgac ttagagttag ctcagggaat ttagggagtt atctgcgacc
     9841 accgaggaac ggcggaatgc caaagaatcc cgatggagct ctagctggcg gttgacaacc
     9901 ccaccttttg gcgtttctgc ggcgttgcag gcgggactgg atacttcgta gaaccagaaa
     9961 ggcaaggcag aacgcgctca gcaagagtgt tggaagtgat agcatgatgt gccttgttaa
    10021 ctaggtcaaa atctgcagta tgcttgatgt tatccaaagt gtgagagagg aaggtccaaa
    10081 catacacgat tgggagaggg cctaggtata agagtttttg agtagaacgc atgtgagccc
    10141 agccatctcg aggagattaa acacgggccg gcatttgatg gctatgttag taccccaatg
    10201 gaaacggtga gagtccagtg gtcgcagata actccctaaa ttccctgagc taactctaag
    10261 tcgaccatgc cgtttatggt tagcgcctcc caaaaaggaa tggccgactt agagttacct
    10321 cttgaccgac tttttctttc ctccccctta catttcgtta ccacaacaca ttcctatatc
    10381 caaactccag gtacataact agtcgaaatc tcttttaaat ctagtcaaga actagatact
    10441 taaccttcat actgcttaga gatagccttt gaagctctta tttcgctctt tgtctcactc
    10501 tcaccttcct cctcctcctc caatcctttt tgctcgggac tagtccaaga ctagtcaacg
    10561 attagtatgc cgagcattcg cgataacgat ctccgaaagt ccccagagta ctgtcactat
    10621 ctcgaggcag ttaaggacgg ggagcttacg ctgccggatt tcaagatagt aagccgaccc
    10681 gactagcttt aagcctagtt actgactagt ccacaggacg acaatggcgt gcctgatatc
    10741 catccatatg aagtctactg ccgagtgaag ggatgcctca agcgtacagt gagtctactg
    10801 ctatactagt ttctgactag ttattaacta gttgacaggt tccctctgcc aacagaaaca
    10861 tattggtcaa gcacttgaag gacaagaact cccacggcat ggagtttaca ttgcacaatg
    10921 gacctcccac tatgaaggaa ctgatggagg ccaaaggcaa gtcctatcta gattacttgg
    10981 tgactagtcc ctgactagtc aagtagcatg gtatgaaggc ttgtttgaag gcactgttct
    11041 cccaaccccg actcctacca agaagcgcaa gcgagctgcg taagtttctg tgagtctaac
    11101 tagtgtatta gctaatatat agcagcacca agtccaagga ccacaatact aagggtgtcg
    11161 agaattcgtg agtttcttct cccatttcaa ctagtccttg actagtcact aactactttg
    11221 cagcaacgag ggtgaagctg gaaatgatca ggacaatggc gagggcccgt aagtacagcc
    11281 attcaatgca gactagttgc taactaatct gtgactagtt caagtggtcc gtacgccgtg
    11341 catacccctg tgactggtag gaatttgagc aagcctgtct tgccgcgcga tgagaaagga
    11401 arggcaagtt acattcagcc ccgtacctag gatcagtgct aatttataac ctctagccac
    11461 tctttatgca gatccgccgt gagggtagca aggcagctaa atcagctggt gagaaaggaa
    11521 ccataccctg caagacctgt cgcaacgcaa agggcaaagg taagctatcc aagctagttt
    11581 gggactagat tctaactagt ctcagcaccg tgtggttcaa agccatattg cgagttttgg
    11641 cgctttttct catcgattga cgaggcaaag ggagcgagta tgcaacctcg taagtcagac
    11701 tcagacaaag accaactagt tattgaccag tcttctagaa ggctctgttg tggatcttga
    11761 ggccctggag agttcctcca acaatccgga gacaagcaag tcctcgtcgg actagtcact
    11821 aactagactc taactagttg cagacatgga taatgcaaaa gagacaagca atgaagaaag
    11881 tggtaagaca tttctccttt gtggttctgg actagtcttt gactagtcac agtcttaaac
    11941 aaggaaaatg agcatgaaaa tgaggaggaa aaggctgctg agcccgagga agtgcagggt
    12001 gatggcagac atggtaggtt aataccttgt tagttattgc tagtcactga ctagtcaata
    12061 actagtctct gaacaccttg caatcactcc gtttgcgcag ctgaacagtg gtgaggataa
    12121 tagtagtaag ttattctagc ttcagagtta taggagacta gatactaact agtattagtt
    12181 gcaactaacc tggatctcag agactttggc ctcaatctag aatctatcta gttgtcaact
    12241 agactgtggt atcattgtct tttattttcc tagtcctgga actagcttct aactagtctc
    12301 cctaatatgt ggctgtcttg tttttttttt ttgtttccct acccggatat ctagtcccct
    12361 tctaggttct gttaacctct cgggctctga tttagtttaa cgcaaacctg agattagttt
    12421 ctaactagtc tctaggtttt ctatccacct ttaattgtaa taataaatac aagcaacgtt
    12481 tatacgtcaa aagcatttat aaacttttac cctaaagtag cttgcttgtg tgtttagttt
    12541 ataattagtc tcttattaat ttgatgtagg taagcccgcc acaaatatat atttttaaca
    12601 agataccgtg gaaaaacttc gtgctatcac aaaacagtat acaaaaaata agct
//
