LOCUS       sequence-483852-       15335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express H3-EGFP and H2B-mRFP1 simultaneously in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15335)
  AUTHORS   Guoliang Yuan, Tatiana Serrano-Zayas, Ziyu Dai, Erica I. DeMers, 
            Shuang Deng, Beth A. Hofstad,, Kyle R. Pomraning
  TITLE     Strong Translation Elongation Factor 1-Alpha (tef1-alpha) Promoter 
            Reporter Systems for Aspergillus niger
  JOURNAL   ACS Synthetic Biology Article ASAP
REFERENCE   2  (bases 1 to 15335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS 
            Synthetic Biology Article ASAP"
COMMENT     Sequence Label: pGY240
FEATURES             Location/Qualifiers
     source          1..15335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1727..1729,1730..2443)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1727..1729,1730..2440)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1769..1790)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2030..2049)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2377..2398
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4404..5078
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIKGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(4531..4549)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4799..4818
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4993..5016
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             5901..6926
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6998..7031)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7501..7517)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7616..7633)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7787..8375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7867..7886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8497..9288,9289..9357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9120..9139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9358..9449)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9491..9510
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9516..9535
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    9742..9775
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      10088..15335
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aaggcccgca ctaagcagac tgcccgtaag tgcaacctca ctgtcctttc ccctcgtgtc
     1201 gttcgaccgc gtcatggtcg cgacacccgt gtctcacatg aggaacatca tcactaacta
     1261 tcatctcaat ataggtaagt ccactggtgg caaggctccc cgtaagcagc tcgcctccaa
     1321 ggctgcccgt aaggccgctc cctccaccgg aggtgtcaag aagcctcacc gttacaagcc
     1381 cggtatgtat acactcatca ttcaaagtgg caaatggcct atccgaacag tcaactaaca
     1441 gtctaccaaa ggtaccgtcg ctctgcgtga aatccgtcgt taccagaaga gcactgagct
     1501 cctgatccgc aagctgccct tccagcgtct tgtccgtgaa attgctcagg acttcaagtc
     1561 ggatctccgc ttccagtcct ccgccatcgg tgctcttcag gagtccgtcg aggcctacct
     1621 cgtctccctc ttcgaggaca ccaacctgtg cgccatccac gccaagcgtg tcaccatcca
     1681 gtccaaggac atccagcttg cccgccgtct ccgtggtgag cgctctgtga gcaagggcga
     1741 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1801 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1861 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1921 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1981 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2041 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2101 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2161 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2221 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2281 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2341 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2401 cgccgccggg atcactctcg gcatggacga gctgtacaag taagcggaca ttcgatttat
     2461 gccgttatga cttccttaaa aaagccttta cgaatgaaag aaatggaatt agacttgtta
     2521 tgtagttgat tctacaatgg attatgattc ctgaacttca aatccgctgt tcattattaa
     2581 tctcagctct tcccgtaaag ccaatgttga aactattcgt aaatgtacct cgttttgcgt
     2641 gtaccttgct tatcacgtga tattacatga cctggacaga gttctgcgcg aaagtcataa
     2701 cgtaaatccc gggcggtagg tgcgtcccgg gcggaaggta gttttctcgt ccaccccaac
     2761 gcgtttatca acctcaactt tcaacaacca tcatgccacc aaaagcgcgt aaaacaaagc
     2821 gagatttgat tgagcaagag ggcaggatcc aatgcgcgat tcaagacatt aaaaatggaa
     2881 aatttcaaaa aattgcgccc gcagcgcgtg catacaaaat tcatcccaat acaagtgtaa
     2941 tgctagtgga ggtccgttga cctcacaggg atttccacca aaaagtggac cgggcgggcg
     3001 ccactcggcc gtgccacagc agcctgagag cggacaaata acaacagccg cctgccgcgg
     3061 ggttcggttg caaacatgac caacaggcca ggccatcatc aacccaccgc tgcgttgatg
     3121 cccaggattt cagtccaata atccacaatt taccaacgga tagagctagg tgaactagat
     3181 agacaggagg gccagaggga ggggaccgag atgaaaaatt ttcgatgaaa gagtggtcaa
     3241 ggtggggtcg tagttcggcg ctccgagggc gaggaaccaa ggaaaggcga gaaaaggaca
     3301 ggctgatcgc gctgcgttgc tgggctgcaa gcgtgtccag ttgagtctgg aaaaggctcc
     3361 gccgtgaaga ttctgcgttg gtcccgcacc tgcgcggtgg gggcattacc cctccatgtc
     3421 caatgatttc aagtcaaagc caagggttga agcccgcccg cttagtcgcc ttctcgcttg
     3481 acccctccat ataagtattt cccctcctcc ccctcccaca aatttttcct ttccctttcc
     3541 tccctcgtcc gcttcagtac gtatatcttc cccccctctc tcttccttct cactcttctc
     3601 tccttctttc ttgattcatc ctctctctaa ctgacttctt tgctcagcac ctctacgcgt
     3661 tctggccgta gtatctgagc aatttttcta cagacttttt ctatctaatt ccaaaaaaga
     3721 acttcgagtt cattcacaac cgtcatcatg gggtgagtca agacaacgat catcatctga
     3781 caaaggaagg acatgggcta acggatatct tctacaacag taagcctccc aaagccgctg
     3841 agaagaagcc cagcaccggt ggcaaggccc cggcaggcgg caaggctccc gctgagaaga
     3901 aggaagccgg caagaagact gctgccgctg cctccggcga caagaagaag cgtggaaaga
     3961 ccaggaagga gacctactcg tcttacatct acaagggtga gtaatcttaa gacttgtatt
     4021 cctagtcaat agttgttgac tgtgttatag tcttgaagca ggtccaccct gataccggta
     4081 tctcgactcg tgctatgtcc atcctgaact cctttgtcaa tggtatgtcg tgaatgaagc
     4141 agggttatac cttcagcaag cacctaacgc ggtctagata tcttcgagcg tgtcgcgacc
     4201 gaggcctcga aacttgccgc ttacaacaag aagtccacca tctcttcacg ggagatccag
     4261 acctcgtaag tcctctgtcg atcaagatag tagcaattcg ctaacacctc tctagtgtga
     4321 ggctcatcct tcccggtgaa ttggcgaagc acgcggtgtc ggaaggcacc aaggctgtca
     4381 cgaagtactc gtcgtctgcc aaagcctcct ccgaggacgt catcaaggag ttcatgcgct
     4441 tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcaag ggcgagggcg
     4501 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
     4561 tgcccttcgc ctgggacatc ctgtcccctc agttccagta cggctccaag gcctacgtga
     4621 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     4681 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     4741 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     4801 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcggatg taccccgagg
     4861 acggcgccct gaagggcgag atcaagatga ggctgaagct gaaggacggc ggccactacg
     4921 acgccgaggt caagaccacc tacatggcca agaagcccgt gcagctgccc ggcgcctaca
     4981 agaccgacat caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     5041 acgagcgcgc cgagggccgc cactccaccg gcgcctaagc ggacattcga tttatgccgt
     5101 tatgacttcc ttaaaaaagc ctttacgaat gaaagaaatg gaattagact tgttatgtag
     5161 ttgattctac aatggattat gattcctgaa cttcaaatcc gctgttcatt attaatctca
     5221 gctcttcccg taaagccaat gttgaaacta ttcgtaaatg tacctcgttt tgcgtgtacc
     5281 ttgcttatca cgtgatatta catgacctgg acagagttct gcgcgaaagt cataacgtaa
     5341 atcccgggcg gtaggtgcgt cccgggcgga aggtagtttt ctcgtccacc ccaacgcgtt
     5401 tatcaacctc aactttcaac aaccatcatg ccaccaaaag cgcgtaaaac aaagcgagat
     5461 ttgattgagc aagagggcag gatccaatgc gcgattcaag acattaaaaa tggaaaattt
     5521 caaaaaattg cgcccgcagc gcgtgcatac aaaattcatc ccaatacaag tgtaatgcta
     5581 gtggaggtca acacatcaat gcctattttg gtttagtcgt ccaggcggtg agcacaaaat
     5641 ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc cacttgtagc
     5701 agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat
     5761 catctgctgc ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa gtggacgacc
     5821 cggtcatacc ttcttaagtt cgcccttcct ccctttattt cagattcaat ctgacttacc
     5881 tattctaccc aagcatccaa atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5941 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     6001 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     6061 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     6121 ttccggaagt gcttgacatt ggggagttca gcgagagcct gacctattgc atctcccgcc
     6181 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctccagc
     6241 cggtcgcgga ggccatggat gcgattgctg cggccgatct tagccagacg agcgggttcg
     6301 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     6361 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     6421 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     6481 tcgtgcatgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     6541 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcctct
     6601 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6661 cggagcttgc aggatcgccg cgcctccggg cgtatatgct ccgcattggt cttgaccaac
     6721 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6781 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6841 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6901 gcactcgtcc gagggcaaag gaatagagta gatgccgacc gatccccact accgcattaa
     6961 gacctcagcg cggccgcaaa tttaaataaa atgaagtgaa gttcctatac tttctagaga
     7021 ataggaactt ctatagtgag tcgaataagg gcgacacaaa atttattcta aatgcataat
     7081 aaatactgat aacatcttat agtttgtatt atattttgta ttatcgttga catgtataat
     7141 tttgatatca aaaactgatt ttccctttat tattttcgag atttattttc ttaattctct
     7201 ttaacaaact agaaatattg tatatacaaa aaatcataaa taatagatga atagtttaat
     7261 tataggtgtt catcaatcga aaaagcaacg tatcttattt aaagtgcgtt gcttttttct
     7321 catttataag gttaaataat tctcatatat caagcaaagt gacaggcgcc cttaaatatt
     7381 ctgacaaatg ctctttccct aaactccccc cataaaaaaa cccgccgaag cgggttttta
     7441 cgttatttgc ggattaacga ttactcgtta tcagaaccgc ccagggggcc cgagcttaag
     7501 actggccgtc gttttacaac acagaaagag tttgtagaaa cgcaaaaagg ccatccgtca
     7561 ggggccttct gcttagtttg atgcctggca gttccctact ctcgccttcc gcttcctcgc
     7621 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7681 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7741 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7801 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7861 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7921 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7981 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8041 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8101 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8161 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtgggctaac tacggctaca
     8221 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8281 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8341 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8401 ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt taagggattt tggtcatgag
     8461 cttgcgccgt cccgtcaagt cagcgtaatg ctctgcttac caatgcttaa tcagtgaggc
     8521 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8581 gataactacg atacgggagg gcttaccatc tggccccagc gctgcgatga taccgcgaga
     8641 accacgctca ccggctccgg atttatcagc aataaaccag ccagccggaa gggccgagcg
     8701 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8761 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat
     8821 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8881 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8941 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cgctacataa
     9001 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9061 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9121 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9181 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9241 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9301 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatatt
     9361 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9421 atttgaatgt atttagaaaa ataaacaaat aggggtcagt gttacaacca attaaccaat
     9481 tctgaacatt atcgcgagcc catttatacc tgaatatggc tcataacacc ccttgtttgc
     9541 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
     9601 gtagcgccga tggtagtgtg gggactcccc atgcgagagt agggaactgc caggcatcaa
     9661 ataaaacgaa aggctcagtc gaaagactgg gcctttcgcc cgggctaatt atggggtgtc
     9721 gcccttattc gactctatag tgaagttcct attctctaga aagtatagga acttctgaag
     9781 tggggattta aatgcggccg cgctgagggt ttaatcgacg aagcagctga cggccagtgc
     9841 caagcttaac gcgtaccggg cccagtatat gttccgcaga tgactggagc tctgccatac
     9901 gtgccctctc aagcaccatt tgttccatct acagagacta gtcaccaact agtctatcaa
     9961 gactcacagg gtacattgct gagaccaact gaccagaggc agggtagcgg attgacggct
    10021 ccatctcctt cacttacaag gtctattgaa agccctttag catcaccaag cggagaatag
    10081 attgttaagc ttattttttg tatactgttt tgtgatagca cgaagttttt ccacggtatc
    10141 ttgttaaaaa tatatatttg tggcgggctt acctacatca aattaataag agactaatta
    10201 taaactaaac acacaagcaa gctactttag ggtaaaagtt tataaatgct tttgacgtat
    10261 aaacgttgct tgtatttatt attacaatta aaggtggata gaaaacctag agactagtta
    10321 gaaactaatc tcaggtttgc gttaaactaa atcagagccc gagaggttaa cagaacctag
    10381 aaggggacta gatatccggg tagggaaaca aaaaaaaaaa caagacagcc acatattagg
    10441 gagactagtt agaagctagt tccaggacta ggaaaataaa agacaatgat accacagtct
    10501 agttgacaac tagatagatt ctagattgag gccaaagtct ctgagatcca ggttagttgc
    10561 aactaatact agttagtatc tagtctccta taactctgaa gctagaataa cttactacta
    10621 ttatcctcac cactgttcag ctgcgcaaac ggagtgattg caaggtgttc agagactagt
    10681 tattgactag tcagtgacta gcaataacta acaaggtatt aacctaccat gtctgccatc
    10741 accctgcact tcctcgggct cagcagcctt ttcctcctca ttttcatgct cattttcctt
    10801 gtttaagact gtgactagtc aaagactagt ccagaaccac aaaggagaaa tgtcttacca
    10861 ctttcttcat tgcttgtctc ttttgcatta tccatgtctg caactagtta gagtctagtt
    10921 agtgactagt ccgacgagga cttgcttgtc tccggattgt tggaggaact ctccagggcc
    10981 tcaagatcca caacagagcc ttctagaaga ctggtcaata actagttggt ctttgtctga
    11041 gtctgactta cgaggttgca tactcgctcc ctttgcctcg tcaatcgatg agaaaaagcg
    11101 ccaaaactcg caatatggct ttgaaccaca cggtgctgag actagttaga atctagtccc
    11161 aaactagctt ggatagctta cctttgccct ttgcgttgcg acaggtcttg cagggtatgg
    11221 ttcctttctc accagctgat ttagctgcct tgctaccctc acggcggatc tgcataaaga
    11281 gtggctagag gttataaatt agcactgatc ctaggtacgg ggctgaatgt aacttgcctt
    11341 tcctttctca tcgcgcggca agacaggctt gctcaaattc ctaccagtca caggggtatg
    11401 cacggcgtac ggaccacttg aactagtcac agattagtta gcaactagtc tgcattgaat
    11461 ggctgtactt acgggccctc gccattgtcc tgatcatttc cagcttcacc ctcgttgctg
    11521 caaagtagtt agtgactagt caaggactag ttgaaatggg agaagaaact cacgaattct
    11581 cgacaccctt agtattgtgg tccttggact tggtgctgct atatattagc taatacacta
    11641 gttagactca cagaaactta cgcagctcgc ttgcgcttct tggtaggagt cggggttggg
    11701 agaacagtgc cttcaaacaa gccttcatac catgctactt gactagtcag ggactagtca
    11761 ccaagtaatc tagataggac ttgcctttgg cctccatcag ttccttcata gtgggaggtc
    11821 cattgtgcaa tgtaaactcc atgccgtggg agttcttatc cttcaagtgc ttgaccaata
    11881 tgtttctgtt ggcagaggga acctgtcaac tagttaataa ctagtcagaa actagtatag
    11941 cagtagactc actgtacgct tgaggcatcc cttcactcgg cagtagactt catatggatg
    12001 gatatcaggc acgccattgt cgtcctgtgg actagtcagt aactaggctt aaagctagtc
    12061 gggtcggctt actatcttga aatccggcag cgtaagctcc ccgtccttaa ctgcctcgag
    12121 atagtgacag tactctgggg actttcggag atcgttatcg cgaatgctcg gcatactaat
    12181 cgttgactag tcttggacta gtcccgagca aaaaggattg gaggaggagg aggaaggtga
    12241 gagtgagaca aagagcgaaa taagagcttc aaaggctatc tctaagcagt atgaaggtta
    12301 agtatctagt tcttgactag atttaaaaga gatttcgact agttatgtac ctggagtttg
    12361 gatataggaa tgtgttgtgg taacgaaatg taagggggag gaaagaaaaa gtcggtcaag
    12421 aggtaactct aagtcggcca ttcctttttg ggaggcgcta accataaacg gcatggtcga
    12481 cttagagtta gctcagggaa tttagggagt tatctgcgac caccgaggaa cggcggaatg
    12541 ccaaagaatc ccgatggagc tctagctggc ggttgacaac cccacctttt ggcgtttctg
    12601 cggcgttgca ggcgggactg gatacttcgt agaaccagaa aggcaaggca gaacgcgctc
    12661 agcaagagtg ttggaagtga tagcatgatg tgccttgtta actaggtcaa aatctgcagt
    12721 atgcttgatg ttatccaaag tgtgagagag gaaggtccaa acatacacga ttgggagagg
    12781 gcctaggtat aagagttttt gagtagaacg catgtgagcc cagccatctc gaggagatta
    12841 aacacgggcc ggcatttgat ggctatgtta gtaccccaat ggaaacggtg agagtccagt
    12901 ggtcgcagat aactccctaa attccctgag ctaactctaa gtcgaccatg ccgtttatgg
    12961 ttagcgcctc ccaaaaagga atggccgact tagagttacc tcttgaccga ctttttcttt
    13021 cctccccctt acatttcgtt accacaacac attcctatat ccaaactcca ggtacataac
    13081 tagtcgaaat ctcttttaaa tctagtcaag aactagatac ttaaccttca tactgcttag
    13141 agatagcctt tgaagctctt atttcgctct ttgtctcact ctcaccttcc tcctcctcct
    13201 ccaatccttt ttgctcggga ctagtccaag actagtcaac gattagtatg ccgagcattc
    13261 gcgataacga tctccgaaag tccccagagt actgtcacta tctcgaggca gttaaggacg
    13321 gggagcttac gctgccggat ttcaagatag taagccgacc cgactagctt taagcctagt
    13381 tactgactag tccacaggac gacaatggcg tgcctgatat ccatccatat gaagtctact
    13441 gccgagtgaa gggatgcctc aagcgtacag tgagtctact gctatactag tttctgacta
    13501 gttattaact agttgacagg ttccctctgc caacagaaac atattggtca agcacttgaa
    13561 ggacaagaac tcccacggca tggagtttac attgcacaat ggacctccca ctatgaagga
    13621 actgatggag gccaaaggca agtcctatct agattacttg gtgactagtc cctgactagt
    13681 caagtagcat ggtatgaagg cttgtttgaa ggcactgttc tcccaacccc gactcctacc
    13741 aagaagcgca agcgagctgc gtaagtttct gtgagtctaa ctagtgtatt agctaatata
    13801 tagcagcacc aagtccaagg accacaatac taagggtgtc gagaattcgt gagtttcttc
    13861 tcccatttca actagtcctt gactagtcac taactacttt gcagcaacga gggtgaagct
    13921 ggaaatgatc aggacaatgg cgagggcccg taagtacagc cattcaatgc agactagttg
    13981 ctaactaatc tgtgactagt tcaagtggtc cgtacgccgt gcatacccct gtgactggta
    14041 ggaatttgag caagcctgtc ttgccgcgcg atgagaaagg aagggcaagt tacattcagc
    14101 cccgtaccta ggatcagtgc taatttataa cctctagcca ctctttatgc agatccgccg
    14161 tgagggtagc aaggcagcta aatcagctgg tgagaaagga accataccct gcaagacctg
    14221 tcgcaacgca aagggcaaag gtaagctatc caagctagtt tgggactaga ttctaactag
    14281 tctcagcacc gtgtggttca aagccatatt gcgagttttg gcgctttttc tcatcgattg
    14341 acgaggcaaa gggagcgagt atgcaacctc gtaagtcaga ctcagacaaa gaccaactag
    14401 ttattgacca gtcttctaga aggctctgtt gtggatcttg aggccctgga gagttcctcc
    14461 aacaatccgg agacaagcaa gtcctcgtcg gactagtcac taactagact ctaactagtt
    14521 gcagacatgg ataatgcaaa agagacaagc aatgaagaaa gtggtaagac atttctcctt
    14581 tgtggttctg gactagtctt tgactagtca cagtcttaaa caaggaaaat gagcatgaaa
    14641 atgaggagga aaaggctgct gagcccgagg aagtgcaggg tgatggcaga catggtaggt
    14701 taataccttg ttagttattg ctagtcactg actagtcaat aactagtctc tgaacacctt
    14761 gcaatcactc cgtttgcgca gctgaacagt ggtgaggata atagtagtaa gttattctag
    14821 cttcagagtt ataggagact agatactaac tagtattagt tgcaactaac ctggatctca
    14881 gagactttgg cctcaatcta gaatctatct agttgtcaac tagactgtgg tatcattgtc
    14941 ttttattttc ctagtcctgg aactagcttc taactagtct ccctaatatg tggctgtctt
    15001 gttttttttt tttgtttccc tacccggata tctagtcccc ttctaggttc tgttaacctc
    15061 tcgggctctg atttagttta acgcaaacct gagattagtt tctaactagt ctctaggttt
    15121 tctatccacc tttaattgta ataataaata caagcaacgt ttatacgtca aaagcattta
    15181 taaactttta ccctaaagta gcttgcttgt gtgtttagtt tataattagt ctcttattaa
    15241 tttgatgtag gtaagcccgc cacaaatata tatttttaac aagataccgt ggaaaaactt
    15301 cgtgctatca caaaacagta tacaaaaaat aagct
//
