LOCUS       sequence-48387-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pEntr-Mef2aflbio
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    160..259
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(304..348)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(361..384)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
       61 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
      121 agagattttg agacacgggc cagagctgca gctggatggc aaataatgat tttattttga
      181 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gctttcttat aatgccaact
      241 ttgtacaaga aagctgggtc tagatatctc gagtgcggcc gcctacctcg agctcggcgc
      301 gccttcgtgc cattcgattt tctgagcctc gaagatgtcg ttcaggccgg acccatcacc
      361 tttatcgtca tcgtctttgt agtcactagt ggtcacccac gcgtccatcc tcattcgctt
      421 tacagaaggg ctttctctgt cctcagtgtt tgggggtcgg ccaagcacaa ttggagaatg
      481 gaagtcgccc cgtggatcct cccgatcact gccatcatag gagctactag agctgctcag
      541 actgtccaca ggggagcgcc ccatttcctg tcggggctgg ggctgcgggg gttgtggctg
      601 gggctgcggt ggtggcggcg gctgctgctg ctgctgctgc tgctgctgct gctggaagcc
      661 cgatggggtc atacgatccc gaggaggtga aatcggttcg gacttgatgc tgatgttttg
      721 gttggtatta atggataaat tggaaccctg agataactgc cctccagcaa caagagagct
      781 ga
//
