LOCUS       sequence-48389-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pEntr-Mef2aflbio
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..185
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(230..274)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(287..310)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
       61 acgggccaga gctgcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg
      121 ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagc
      181 tgggtctaga tatctcgagt gcggccgcct acctcgagct cggcgcgcct tcgtgccatt
      241 cgattttctg agcctcgaag atgtcgttca ggccggaccc atcaccttta tcgtcatcgt
      301 ctttgtagtc actagtggtc acccacgcgt ccatcctcat tcgctttaca gaagggcttt
      361 ctctgtcctc agtgtttggg ggtcggccaa gcacaattgg agaatggaag tcgccccgtg
      421 gatcctcccg atcactgcca tcataggagc tactagagct gctcagactg tccacagggg
      481 agcgccccat ttcctgtcgg ggctggggct gcgggggttg tggctggggc tgcggtggtg
      541 gcggcggctg ctgctgctgc tgctgctgct gctgctgctg gaagcccgat ggggtcatac
      601 gatcccgagg aggtgaaatc ggttcggact tgatgctgat gttttggttg gtattaatgg
      661 ataaattgga accctgagat aactgccctc cagcaacaag agagctgagg gctgcttgtc
      721 ctaggtggtg ctgctgccag gccgacacct gtcccagcga cagcattcct ggcgagttga
      781 agccttgaag ggctgacagg tcagcgctgg tcagtgaata atcagtgttg taggcagtcg
      841 g
//
