LOCUS       sequence-48391-e        6762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Nabhan JF, Hu R, Oh RS, Cohen SN, Lu Q
  TITLE     Formation and release of arrestin domain-containing protein 
            1-mediated microvesicles (ARMMs) at plasma membrane by recruitment 
            of TSG101 protein.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4146-51. Epub 2012 Feb
            6.
  PUBMED    22315426
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-03-13"; volume: "109(11)"; pages: 
            "4146-51. Epub 2012 Feb 6"
COMMENT     Sequence Label: pCI-neo.ARRDC1
FEATURES             Location/Qualifiers
     source          1..6762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    62..183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(73..92)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     127..146
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      369..824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(456..475)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     666..687
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        941..1298
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        941..1132
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(964..984)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1149..1284
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1349..2143
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1403..1422)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2013..2032
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2207..2255
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(2296..2315)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2415..2437
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2475..2493)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2561..2665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2666..2734,2735..3526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2884..2903)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3697..4285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4186..4205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4496..4875
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4876..5087
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5038..5058
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5084..5108
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5248..5380
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5363..5382
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5425..5443
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagagtcga cccgggcggc cgcttccctt tagtgagggt taatgcttcg agcagacatg
       61 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      121 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      181 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
      241 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcc gataaggatc gatccgggct
      301 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      361 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      421 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      481 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      541 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      601 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      661 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
      721 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
      781 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
      841 ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca gcaccatggc
      901 ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc
      961 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     1081 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     1141 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     1201 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     1261 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttgattct tctgacacaa
     1321 cagtctcgaa cttaaggcta gagccaccat gattgaacaa gatggattgc acgcaggttc
     1381 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     1441 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     1501 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     1561 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     1621 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     1681 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     1741 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     1801 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     1861 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     1921 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     1981 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     2041 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     2101 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     2161 gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt
     2221 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatccgcgt
     2281 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     2341 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2401 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2461 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2521 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2581 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2641 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2701 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2761 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2821 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2881 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2941 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3001 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3061 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3121 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3181 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3241 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3301 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3361 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3421 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3481 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3541 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3601 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3661 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3721 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3781 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3841 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3901 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3961 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4021 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4081 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4141 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4201 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4261 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4321 cttttgctgg ccttttgctc acatggctcg acagatcttc aatattggcc attagccata
     4381 ttattcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
     4441 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttggcat
     4501 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     4561 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     4621 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     4681 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     4741 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     4801 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
     4861 atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt
     4921 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4981 caaaatcaac gggactttcc aaaatgtcgt aacaactgcg atcgcccgcc ccgttgacgc
     5041 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     5101 gtcagatcac tagaagcttt attgcggtag tttatcacag ttaaattgct aacgcagtca
     5161 gtgcttctga cacaacagtc tcgaacttaa gctgcagtga ctctcttaag gtagccttgc
     5221 agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga
     5281 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
     5341 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat
     5401 tacagctctt aaggctagag tacttaatac gactcactat aggctagcct cgagaattca
     5461 tggggcgagt gcagctcttc gagatcagcc tgagccacgg ccgcgtcgtc tacagccccg
     5521 gggagccgtt ggctgggacc gtgcgcgtgc gcctgggggc accgctgccg ttccgagcca
     5581 tccgggtgac ctgcataggt tcctgcgggg tctccaacaa ggctaatgac acagcgtggg
     5641 tagtggagga gggttacttc aacagttccc tgtcgctggc agacaagggg agcctgcccg
     5701 ctggagagca cagcttcccc ttccagttcc tgcttcctgc cactgcaccc acgtcctttg
     5761 agggtccttt cgggaagatc gtgcaccagg tgagggccgc catccacacg ccacggtttt
     5821 ccaaggatca caagtgcagc ctcgtgttct atatcttgag ccccttgaac ctgaacagca
     5881 tcccagacat tgagcaaccc aacgtggcct ctgccaccaa gaagttctcc tacaagctgg
     5941 tgaagacggg cagcgtggtc ctcacagcca gcactgatct ccgcggctat gtggtggggc
     6001 aggcactgca gctgcatgcc gacgttgaga accagtcagg caaggacacc agccctgtgg
     6061 tggccagtct gctgcagaaa gtgtcctata aggccaagcg ctggatccac gacgtacgga
     6121 ccattgcgga ggtggagggt gcgggcgtca aggcctggcg gcgggcgcag tggcacgagc
     6181 agatcctggt gcctgccttg ccccagtcgg ccctgccggg ctgcagcctc atccacatcg
     6241 actactactt acaggtctct ctgaaggcgc cggaagctac tgtgaccctc ccggtcttca
     6301 ttggcaatat tgctgtgaac catgccccag tgagcccccg gccaggcctg gggctgcctc
     6361 ctggggcccc acccctggtg gtgccttccg caccacccca ggaggaggct gaggctgagg
     6421 ctgcggctgg cggcccccac ttcttggacc ccgtcttcct ctccaccaag agccattcgc
     6481 agcggcagcc cctgctggcc accttgagtt ctgtgcctgg tgcgccggag ccctgccctc
     6541 aggatggcag ccctgcctca cacccgctgc accctccctt gtgcatttca acaggtgcca
     6601 ctgtccccta ctttgcagag ggctccgggg ggccagtgcc cactaccagc accttgattc
     6661 ttcctccaga gtacagttct tggggctacc cctatgaggc cccaccgtct tatgagcaga
     6721 gctgcggcgg cgtggaaccc agcctgaccc ctgagagctg at
//
