LOCUS       sequence-483943-        1259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human PHF13 (100-200) with Flag-tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pFlag-CMV4-PHF13 (100-200)
FEATURES             Location/Qualifiers
     source          1..1259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    413..1035
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(612..631)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(1059..1079)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ctattagcag agctcgttta gtgaaccgtc agaattaatt cagatcgatc taccatggac
       61 tacaaagacg atgacgacaa gcttgcggcc gcgaattcga tggccaagcc cagtaacttc
      121 ctgctggaca gaaagaaaac ggacaagctg aagaagaaga agaagaggaa gcgcagggac
      181 agtgatgcgc ctgggaaaga ggggtacagg gggggcttgc tgaagctgga agccgctgac
      241 ccctacgtgg agacccccac gagtcccacc ttgcaggata tcccccaggc tcccagcgac
      301 ccctgctcgg gctgggactc cgatactccc tcgagtggat cttgtgccac tgtgtcacct
      361 gatcaggtca aagaaataaa aactgaaggc aaacggacta tctgaggatc ccgggtggca
      421 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
      481 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
      541 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
      601 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
      661 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
      721 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
      781 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
      841 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa
      901 ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc
      961 ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta
     1021 gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc agttagggtg
     1081 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     1141 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     1201 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccattccgc ccctaaatc
//
