LOCUS       sequence-483949-        1236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human PHF13 N terminal domain fused to mEYFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pEYFP-C1-PHF13_NTD (21-70)
FEATURES             Location/Qualifiers
     source          1..1236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(60..62,63..65,66..776)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(105..126)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(366..385)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     713..734
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    777..842
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1128..1209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1165..1184)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcttatagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca
       61 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      121 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      181 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      241 tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
      301 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      361 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      421 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      481 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
      541 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      601 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      661 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      721 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
      781 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
      841 ccatgaggcg caggaccgtg gaagacttca acaaattctg cacctttgtc ttggcctatg
      901 ctggctacat cccttatccg aaggaggaac tccctttaag gagcagcccc agccctgcta
      961 acagcactgc tggtaccatt gacagcgacg gctggtgagg atccaccgga tctagataac
     1021 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     1081 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1141 cagcttataa gggttacaaa taaagcaata gcatcacaaa tttcccaaat aaagcatttt
     1201 tttcactgca ttctagttgg ggtttgtcca aactca
//
