LOCUS       sequence-483985-        1191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human PHF13 with PEST1 deletion and N terminal tet 
            operator in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pCDNA-4TO-PHF13∆PEST1(del52-88)
FEATURES             Location/Qualifiers
     source          1..1191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     37..61
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(1038..1055)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tgtaggtctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
       61 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      121 cctccggact ctagcgttta aacttaagct tggtaccgag ctcggatcca tggactctga
      181 ctcttgcgcc gccgccttcc acccggagga atactccccc agttgcaaga ggcgcaggac
      241 cgtggaagac ttcaacaaat tctgcacctt tgtcttggcc tatgctggct acatccctta
      301 tccgaaggag gaactccctt tacgctgctt tagccacctg cagcctactc tcttgcagcg
      361 agccaagccc agtaacttcc tgctggacag aaagaaaacg gacaagctga agaagaagaa
      421 gaagaggaag cgcagggaca gtgatgcgcc tgggaaagag gggtacaggg ggggcttgct
      481 gaagctggaa gccgctgacc cctacgtgga gacccccacg agtcccacct tgcaggatat
      541 cccccaggct cccagcgacc cctgctcggg ctgggactcc gatactccct cgagtggatc
      601 ttgtgccact gtgtcacctg atcaggtcaa agaaataaaa actgaaggca aacggactat
      661 cgtccggcag ggaaagcagg tggtgttccg agatgaggac agcactggca atgatgagga
      721 catcatggtg gactcagatg acgattcctg ggacctcgtg acctgcttct gcatgaagcc
      781 atttgccggc cgccccatga tcgagtgtaa tgagtgccac acctggattc acctgtcctg
      841 tgcgaaaatc cggaaatcca atgttccaga agtgtttgtc tgccaaaagt gccgggactc
      901 caagtttgac atccgccgtt ccaaccgctc gcggacgggc tcccggaagc tgttcctgga
      961 ctgaccggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt
     1021 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     1081 ctcccccgtg ccttccttga ccctggaagg tgccctccac tgtcctttcc taataaatga
     1141 ggaattgcat cgcatgtctg agtaggtgtc atctatctgg gggggggggg g
//
