LOCUS       sequence-484327-         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  263-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern 
            in the presence of ZFa and editable by Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: (263-bp-DIAL-YB_TATA)-mGL-BGH_pKG2847
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(123..156)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    274..298
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     353..372
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(420..453)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agatctgaat tggagtgagg acgtgtagag tgaggacgtg cggagtgagg acgtgtagag
       61 tgaggacgtg cggagtgagg acgtgtagag tgaggacgtg ccctcctctg gatcaaatcc
      121 gaataacttc gtatagcata cattatacga agttatctcg agtcgctcgg tacgatttaa
      181 attgaattct gcagtcgacg gtaccgcggg cccgggatcc tgatagtgac ctgttcgttg
      241 caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa agcaggctcc
      301 accatgggaa ccaattcagt cgactggatc cggtacctcc tacagctcct gggcaacgtg
      361 ctggttattg tgctgtctca tcattttggc aaagaattga ttaattcgag cgaacgcgta
      421 taacttcgta tagcatacat tatacgaagt tatcggattt gatctgatca agcaactcta
      481 gagggtatat aatgggggcc actagtctac taccagagct catcgctagc
//
