LOCUS       sequence-484333-         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  203-bp DIAL Reporter Plasmid with SV40 expressing mGreenLantern in 
            the presence of ZFa and editable by Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: (203-bp-DIAL-SV40)-mGL-BGH_pKG3687
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(123..156)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     293..312
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(360..393)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        421..617
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      468..603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agatctgaat tggagtgagg acgtgtagag tgaggacgtg cggagtgagg acgtgtagag
       61 tgaggacgtg cggagtgagg acgtgtagag tgaggacgtg ccctcctctg gatcaaatcc
      121 gaataacttc gtatagcata cattatacga agttatctcg agtcgctcgg tacgatttaa
      181 attgaattct gcagtcgacg gtaccgcggg cccgggactt tgtacaaaaa agcaggctcc
      241 accatgggaa ccaattcagt cgactggatc cggtacctcc tacagctcct gggcaacgtg
      301 ctggttattg tgctgtctca tcattttggc aaagaattga ttaattcgag cgaacgcgta
      361 taacttcgta tagcatacat tatacgaagt tatcggattt gatctgatca agcaactagt
      421 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
      481 ctccgcccag ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag
      541 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
      601 gcctaggctt ttgcaaaaag cttaatccgc tagtctacta ccagagctca tcgctagc
//
