LOCUS       sequence-484341-         521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  203-bp DIAL Reporter Plasmid with CMV53 expressing mGreenLantern in 
            the presence of ZFa and editable by Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: (203-bp-DIAL-CMV53)-mGL-BGH_pKG3682
FEATURES             Location/Qualifiers
     source          1..521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(123..156)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     293..312
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(360..393)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        434..478
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 agatctgaat tggagtgagg acgtgtagag tgaggacgtg cggagtgagg acgtgtagag
       61 tgaggacgtg cggagtgagg acgtgtagag tgaggacgtg ccctcctctg gatcaaatcc
      121 gaataacttc gtatagcata cattatacga agttatctcg agtcgctcgg tacgatttaa
      181 attgaattct gcagtcgacg gtaccgcggg cccgggactt tgtacaaaaa agcaggctcc
      241 accatgggaa ccaattcagt cgactggatc cggtacctcc tacagctcct gggcaacgtg
      301 ctggttattg tgctgtctca tcattttggc aaagaattga ttaattcgag cgaacgcgta
      361 taacttcgta tagcatacat tatacgaagt tatcggattt gatctgatca agcaaccaac
      421 aaaatgtcgt aacaagggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      481 tttagtgaac cgctagtcta ctaccagagc tcatcgctag c
//
