LOCUS       sequence-484353-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  380-bp DIAL (YB_TATA) Reporter Lentivirus (with divergent SNAP) 
            expressing mCherry-HRasG12V in the presence of ZFa and editable by 
            Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: 
            LentiX_((380-bp-DIAL)-mCh-HRasG12V-BGH)rev_DIV_pKG3848
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(154..187)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(288..307)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(521..540)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    complement(568..601)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 attggagtga ggacgtgtag agtgaggacg tgcggagtga ggacgtgtag agtgaggacg
       61 tgcggagtga ggacgtgtag agtgaggacg tgccctcctc tggatcaaat ccgaattcgc
      121 tacataccct cgtaaactct ggatcaaatc cgaataactt cgtatagcat acattatacg
      181 aagttatctc gagtcgctcg gtacgattta aattgaattc tgcagtcgac ggtaccgcgg
      241 gcccgggact gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
      301 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
      361 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
      421 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
      481 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
      541 catagttaag ccagccccga cacataaata acttcgtata gcatacatta tacgaagtta
      601 tcggatttga tctgatcaag caagccactc taagcattat agtctctaga gggtatataa
      661 tgggggccac tagtctacta ccagagctca tcgctagc
//
