LOCUS       sequence-484387-         506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  loxPcon TET-DIAL Reporter Lentivirus with YB_TATA expressing 
            mCherry-HRasG12V in the presence of DOX and rtTA and editable by Cre
            recombinase. Contains rtTA-TagBFP expressed divergenty..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: 
            LentiX-(loxPcon-TET-DIAL-YB-TATA)-mCherry-HRasG12V-bGH-EFS-rtTA-TagB
            FP-WPRE
FEATURES             Location/Qualifiers
     source          1..506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    78..111
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(128..506)
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     primer_bind     complement(195..219)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    264..282
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    300..318
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    336..354
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    372..390
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    408..426
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    444..462
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    480..498
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gctagcgatg agctctggta gtagactagt ggcccccatt atataccctc tagagttgct
       61 tgatcagatc aaatccgata acttcgtata atgtatgcta tacgaagtta tacgcgttcg
      121 ctcgaatttt acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg
      181 gaattgctcc aggcgatctg acggttcact aaacgagctc tgcttttata ggcgcccacc
      241 gtacacgcct aaagcttata cgttctctat cactgatagg gagtaaactg gatatacgtt
      301 ctctatcact gatagggagt aaactgtaga tacgttctct atcactgata gggagtaaac
      361 tggtcatacg ttctctatca ctgataggga gtaaactcct tatacgttct ctatcactga
      421 tagggagtaa agtctgcata cgttctctat cactgatagg gagtaaactc ttcatacgtt
      481 ctctatcact gatagggagt aaactc
//
