LOCUS       sequence-484395-        1524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for rtTA-2A-tagBFP driven from the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: CAG-rtTA-2A-tagBFP-bGH_pKG3264
FEATURES             Location/Qualifiers
     source          1..1524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..744
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             754..810
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             823..1524
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 atgagccggc tggacaagag caaagtgatc aacagcgccc tggaactgct gaacggcgtg
       61 ggcatcgagg gcctgaccac ccggaagctg gcccagaaac tgggcgtgga acagcccacc
      121 ctgtactggc acgtgaagaa caagcgggcc ctgctggacg ccctgcccat cgagatgctg
      181 gaccggcacc acacccacag ctgccctctg gaaggcgaga gctggcagga cttcctgcgg
      241 aacaacgcca agagctacag atgcgccctg ctgagccacc gggacggcgc caaagtgcac
      301 ctgggcacca gacccaccga gaagcagtac gagacactgg aaaaccagct ggccttcctg
      361 tgccagcagg gcttcagcct ggaaaacgcc ctgtacgccc tgagcgccgt gggccacttc
      421 accctgggct gcgtgctgga agaacaggaa caccaggtcg ccaaagagga aagagagaca
      481 cccaccaccg acagcatgcc ccccctgctg aagcaggcca tcgagctgtt cgacagacag
      541 ggcgccgagc ccgccttcct gttcggcctg gaactgatca tctgcggcct cgagaagcag
      601 ctgaagtgcg agagcggcgg acccaccgac gccctggacg acttcgacct ggacatgctg
      661 cccgccgatg ccctggatga ttttgatctg gatatgctcc ctgctgacgc tctcgatgac
      721 ttcgatctcg atatgctgcc aggcggaagc ggagctacta acttcagcct gctgaagcag
      781 gctggagacg tggaggagaa ccctggacct gcaccaggaa gtatgagcga gctgattaag
      841 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
      901 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
      961 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     1021 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     1081 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     1141 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     1201 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg
     1261 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     1321 gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac
     1381 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     1441 aacgaaacgt acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
     1501 aaactggggc acaagcttaa ttaa
//
