LOCUS       sequence-484465-       12889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 12889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRQ(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..12889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             8494..8529
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             8539..8562
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             10222..10266
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             10267..10293
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(10433..10450)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10439..10550
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10668..10688)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10673..11002
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10853..10988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10915..10934
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11050..11097
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11116..11715
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11116..11135)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11612..11632
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    11845..11966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11882..11901)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11936..11955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(12019..12810,12811..12879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12642..12661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(12880..12889,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga gagatatcaa
     2761 gtggatctgg tgcctgacag cggcttcgtg accattagag atctcggcgg aggcgagtat
     2821 caggtggacg tgtcaaatgt ggtgcccggc acaagatacg acatcaccat cagcagcatc
     2881 agcaccacct acacaagccc cgtgaccaga atcgtgacca ccaacgtgac aaagcccgga
     2941 cctcctgtgt ttctggctgg cgaaagagtg ggatctgccg gcatcctgct gagctggaat
     3001 acccctccta atcctaacgg ccggatcatc agctacatcg tgaagtacaa agaagtgtgc
     3061 ccctggatgc agaccgtgta cacacaagtg cggagcaagc ccgatagcct ggaagtgctg
     3121 ctgaccaacc tgaatcctgg cacaacctac gagatcaagg tggccgccga aaattctgcc
     3181 ggaatcggcg tgttcagcga cccctttctg ttccaaactg ccgagtctgc ccctggcaag
     3241 gtggtcaatc tgaccgtgga agcctacaac gccagcgccg tgaagctgat ctggtatctg
     3301 cctagacagc ccaacggcaa gatcaccagc ttcaagatca gcgtgaagca cgctagaagc
     3361 ggcatcgtgg tcaaggacgt gtccatcaga gtggaagata tcctgaccgg caagctgccc
     3421 gagtgcaacg agaatagcga gagcttcctg tggtccaccg cctctccatc tccaacactg
     3481 ggaagagtga cccctccaag cagaacaacc cacagcagca gcaccctgac acagaacgag
     3541 atcagcagcg tgtggaaaga gcccatcagc ttcgtcgtga cccacctgag gccttacacc
     3601 acatacctgt ttgaggtgtc cgccgtgaca accgaggccg gctacatcga ttctacaatc
     3661 gtgcggaccc ctgagtctgt gcctgaggga cctcctcaga actgcgtgac aggcaatatc
     3721 accggcaaga gcttcagcat cctgtgggac cctccaacca tcgtgaccgg aaagttcagc
     3781 tatagagtgg aactgtacgg ccccagcggc agaatcctgg ataacagcac caaggacctg
     3841 aagttcgcct tcaccaatct gacacccttc actatgtacg atgtgtatat cgccgctgag
     3901 acaagcgccg gcacaggccc taagagcaac atctctgtgt tcacccctcc agatgtgcct
     3961 ggcgccgtgt ttgatctgca gctggccgag gtggaaagca cccaagttcg gatcacctgg
     4021 aagaagccac ggcagcctaa cggcatcatc aaccagtata gagtgaaggt gctggtgccc
     4081 gagacaggca tcatcctgga aaacaccctg ctgaccggga acaacgagta catcaacgac
     4141 cccatggctc ccgagatcgt gaacatcgtg gaacccatgg tcggactgta tgagggctct
     4201 gccgagatgt ctagcgatct gcacagcctg gccacattca tctacaactc tcaccccgac
     4261 aagaacttcc ccgccagaaa tagagccgag gaccagacaa gccctgtggt caccaccaga
     4321 aaccagtaca tcaccgatat tgccgccgag cagctgagct acgtgatcag acgactggtg
     4381 cccttcaccg agcacatgat ctccgtgtcc gcctttacca tcatgggcga aggacctcca
     4441 acagtgctga gcgtgcgaac aagacagcag gtccccagct ccatcaagat catcaattac
     4501 aagaacatct ccagctcctc catcctgctc tactgggacc caccagagta ccctaatgga
     4561 aagatcaccc actacaccat ctacgccatg gaactggaca ccaaccgggc cttccagatc
     4621 acaaccatcg acaacagctt tctgatcacc ggcctgaaga agtacaccaa gtacaagatg
     4681 agagtggccg ccagcacaca cgtgggagag tctagcctga gcgaagagaa cgacatcttt
     4741 gtgcggacca gcgaggacga gcctgaaagc tctcctcagg acgtggaagt gatcgacgtg
     4801 accgccgatg agatcagact gaagtggtcc ccacctgaaa agcccaatgg catcattatc
     4861 gcctacgagg tgctgtacaa gaatatcgac accctgtaca tgaagaacac ctccaccacc
     4921 gatatcatcc tgcggaacct gcggcctcac acactgtaca atatcagcgt gcggagctac
     4981 accagattcg gccacggaaa tcaggtgtcc tctctgctgt ccgtgcggac atctgagaca
     5041 gtgccagata gcgcccctga gaacatcacc tataagaaca tcagctccgg cgagatcgag
     5101 ctgtcctttc tgcctcctag ctctcccaac gggatcatta agaagtatac catctacctg
     5161 aagcggagca acggcaacga ggaacggacc atcaatacca ccagcctgac tcagaacatc
     5221 aaggtgctca aaaagtacac gcagtatatc atcgaagtgt ccgcaagcac actgaaaggc
     5281 gagggcgtta gaagcgcccc tatcagcatt ctgaccgaag aggatgcccc tgacagccct
     5341 ccacaggatt tttccgtgaa gcagctgtcc ggcgtgaccg tgaaactgtc ttggcaacct
     5401 cctctggaac ccaacggaat tatcctgtac tacaccgtgt acgtgtggaa ccggtccagc
     5461 ctgaaaacca tcaatgtgac cgaaaccagc ctggaactga gcgacctgga ctacaacgtg
     5521 gaatactccg cctacgtgac agccagcaca agatttggcg acggcaagac cagaagcaac
     5581 atcattagct ttcagacccc tgaaggcgcc cctagcgatc ctcctaagga tgtgtactac
     5641 gccaacctga gcagctccag catcattctg ttctggaccc cacctagcaa gccaaatggg
     5701 atcatccagt actatagcgt gtactaccgg aacaccagcg gcaccttcat gcagaacttc
     5761 accctgcacg aagtgaccaa cgacttcgac aacatgaccg tgtccaccat catcgacaag
     5821 ctgaccatct tcagctacta caccttctgg ctgaccgcct ccacaagcgt cggcaatgga
     5881 aacaagagca gcgacatcat cgaggtgtac acagaccagg acatccctga gggctttgtg
     5941 ggcaacctga cctacgagtc tatcagctcc accgccatca acgtgtcatg ggtgccacca
     6001 gcacagccta atggcctggt gttctactac gtgtccctga tcctgcagca gacccctaga
     6061 catgttcggc ctccactggt cacatacgag cggagcatct acttcgataa cctggaaaag
     6121 tataccgact acatcctgaa gatcacaccc agcaccgaga agggcttcag cgacacatac
     6181 acagcccagc tgtacatcaa gactgaagag gacgtgcccg aaacaagccc tatcatcaac
     6241 accttcaaga acctgtccag caccagcgtg ctgctttctt gggacccgcc tgtgaaacct
     6301 aacggcgcca tcatctccta cgacctgaca ctgcagggcc ccaacgagaa ctacagcttc
     6361 atcaccagcg acaactacat catcctcgaa gaactgagcc cctttacgct gtactccttc
     6421 tttgccgccg ctcggacaag aaaaggcctg ggacctagct ctatcctgtt cttctacacc
     6481 gacgagagcg tgccactggc tcctccacag aatctgaccc tgatcaactg cacctccgac
     6541 ttcgtgtggc tcaaatggtc cccttctcct ctgcctggcg gaatcgtgaa ggtgtacagc
     6601 tttaagatcc acgagcacga gacagacacg atctactaca aaaacatcag cgggttcaag
     6661 accgaggcca agctcgttgg actggaacct gtgtccacct actctatcag agtgtccgct
     6721 ttcaccaaag tcggaaacgg caaccagttc tccaacgtcg tgaagttcac cacacaagag
     6781 tccgtgccag acgtggtgca gaacatgcag tgtatggcca caagctggca gagcgtgctg
     6841 gtcaaatggg accctcctaa gaaggccaac ggaatcatca cacagtacat ggtcaccgtg
     6901 gaacggaact ccaccaaggt gtcaccccag gaccacatgt ataccttcat caagctgctg
     6961 gccaacacca gctacgtgtt caaagtgcgg gcctctacat ctgctggcga aggcgacgag
     7021 tccacctgtc atgtgtctac cctgcctgaa accgtgccta gcgtgcccac caatatcgcc
     7081 ttttccgatg tgcagagcac ctccgccaca ctgacatgga tcagacctga taccatcctg
     7141 ggctacttcc agaactacaa gattaccaca cagctgcggg cccagaaatg caaagaatgg
     7201 gagtccgagg aatgcgtcga gtaccagaag atccagtacc tgtacgaggc ccacctgacc
     7261 gaggaaacag tgtacggact gaagaaattc cggtggtaca gattccaggt ggcagcctct
     7321 acaaacgccg gctacggcaa tgccagcaac tggatcagca caaagaccct gccagggcct
     7381 cctgatggcc ctcctgaaaa tgtgcatgtg gtggctacaa gccctttcag catctctatc
     7441 tcttggagcg agcccgccgt gatcacaggc ccaacatgtt acctgatcga tgtgaagtcc
     7501 gtggacaacg acgagttcaa catctccttt atcaagagca atgaggaaaa caagaccatc
     7561 gagattaagg acctcgagat cttcacccgg tacagcgtgg tcatcaccgc cttcactgga
     7621 aacatctccg ccgcctatgt ggaaggcaag tccagcgccg agatgatcgt gactacactg
     7681 gaaagcgccc caaaggaccc acctaacaat atgaccttcc agaaaatccc cgacgaagtc
     7741 acaaagttcc agctgacctt cctgccacca agccagccaa acggaaacat tcaggtgtac
     7801 caggctctgg tgtacagaga ggatgatcct accgccgtgc agatccacaa tctgtccatc
     7861 atccagaaaa cgaacacctt cgtgatcgcc atgctcgagg gactcaaagg cggccacacc
     7921 tacaacattt ccgtgtacgc cgtgaatagc gctggcgccg gacctaaggt gcccatgaga
     7981 atcaccatgg acatcaaggc ccctgccaga cctaagacca agccaacacc tatctacgac
     8041 gccaccggaa agctgctcgt gacaagcacc accatcacaa tcagaatgcc catctgctac
     8101 tacagcgacg atcacggccc catcaagaac gtgcaggtcc tggttacaga aacaggcgcc
     8161 cagcacgatg gcaacgtgac caagtggtac gacgcctact tcaacaaggc cagaccttac
     8221 ttcaccaacg agggcttccc caatcctcct tgtaccgagg gaaagaccaa gttcagcggc
     8281 aacgaagaga tctacatcat tggcgccgac aacgcctgca tgatccccgg aaatgaggac
     8341 aagatctgca acggccctct gaagcccaag aagcagtatc tgttcaagtt ccgggccacc
     8401 aacatcatgg gacagttcac cgacagcgac tactctgacc cagtgaaaac actcggcgag
     8461 ggcctgtctg agagaaccgt tgaaggcgcg ccggaagatc aagttgatcc aagattgatt
     8521 gatggcaagg gcggatctct ggaggtgctg ttccagggac caggcggaag cggcaatccc
     8581 ggcggcgatc tggcccccca gatgctgcgc gagctgcagg agaccaatgc cgccctgcag
     8641 gacgtgcgcg agctgctgcg ccaccgcgtg aaggagatca ccttcctgaa gaacaccgtg
     8701 atggagtgcg acgcctgcgg tatgcagccc tctagaggct ccggctccat catcccagtt
     8761 gaggaggaga acccggactt ctggaaccgc gaggcagccg aggccctggg tgccgccaag
     8821 aagctgcagc ctgcacagac agccgccaag aacctcatca tcttcctggg cgatgggatg
     8881 ggggtgtcta cggtgacagc tgccaggatc ctaaaagggc agaagaagga caaactgggg
     8941 cctgagatac ccctggccat ggaccgcttc ccatatgtgg ctctgtccaa gacatacaat
     9001 gtagacaaac atgtgccaga cagtggagcc acagccacgg cctacctgtg cggggtcaag
     9061 ggcaacttcc agaccattgg cttgagtgca gccgcccgct ttaaccagtg caacacgaca
     9121 cgcggcaacg aggtcatctc cgtgatgaat cgggccaaga aagcagggaa gtcagtggga
     9181 gtggtaacca ccacacgagt gcagcacgcc tcgccagccg gcacctacgc ccacacggtg
     9241 aaccgcaact ggtactcgga cgccgacgtg cctgcctcgg cccgccagga ggggtgccag
     9301 gacatcgcta cgcagctcat ctccaacatg gacattgacg tgatcctagg tggaggccga
     9361 aagtacatgt ttcgcatggg aaccccagac cctgagtacc cagatgacta cagccaaggt
     9421 gggaccaggc tggacgggaa gaatctggtg caggaatggc tggcgaagcg ccagggtgcc
     9481 cggtatgtgt ggaaccgcac tgagctcatg caggcttccc tggacccgtc tgtgacccat
     9541 ctcatgggtc tctttgagcc tggagacatg aaatacgaga tccaccgaga ctccacactg
     9601 gacccctccc tgatggagat gacagaggct gccctgcgcc tgctgagcag gaacccccgc
     9661 ggcttcttcc tcttcgtgga gggtggtcgc atcgaccatg gtcatcatga aagcagggct
     9721 taccgggcac tgactgagac gatcatgttc gacgacgcca ttgagagggc gggccagctc
     9781 accagcgagg aggacacgct gagcctcgtc actgccgacc actcccacgt cttctccttc
     9841 ggaggctacc ccctgcgagg gagctccatc ttcgggctgg cccctggcaa ggcccgggac
     9901 aggaaggcct acacggtcct cctatacgga aacggtccag gctatgtgct caaggacggc
     9961 gcccggccgg atgttaccga gagcgagagc gggagccccg agtatcggca gcagtcagca
    10021 gtgcccctgg acgaagagac ccacgcaggc gaggacgtgg cggtgttcgc gcgcggcccg
    10081 caggcgcacc tggttcacgg cgtgcaggag cagaccttca tagcgcacgt catggccttc
    10141 gccgcctgcc tggagcccta caccgcctgc gacctggcgc cccccgccgg caccaccgac
    10201 gccgcgcacc cgggtggcgg aggactgaac gacatcttcg aggctcagaa aatcgaatgg
    10261 cacgaacatc accatcacca tcaccatcac cattaaggcc ggcctgtagt cactggcaca
    10321 acgcgttacc cggtaagcca atcgggtata cacggtcgtc atactgcaga cagggttctt
    10381 ctactttgca agatagtctt gagtagtaaa ataaatagat agagaaaatc agcctcgact
    10441 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
    10501 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc acaacactca
    10561 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    10621 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
    10681 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    10741 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10801 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    10861 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10921 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
    10981 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
    11041 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
    11101 gtgaggaact aaaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
    11161 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
    11221 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
    11281 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
    11341 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
    11401 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
    11461 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
    11521 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
    11581 gtgcccgcct tcctggagac atccgcgccc cgcaacctcc ccttctacga gcggctcggc
    11641 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
    11701 aagcccggtg cctgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
    11761 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    11821 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
    11881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    11941 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    12001 ttggcgtaat catggtcatt accaatgctt aatcagtgag gcacctatct cagcgatctg
    12061 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    12121 gggcttacca tctggcccca gcgctgcgat gataccgcga gaaccacgct caccggctcc
    12181 ggatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    12241 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    12301 agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc
    12361 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    12421 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    12481 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    12541 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    12601 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    12661 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    12721 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    12781 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    12841 aaagggaata agggcgacac ggaaatgttg aatactcata ttcttcctt
//
