LOCUS       sequence-484565-       12622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning gene libraries from oligonucleotide pools.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Dvir G, Xing Z, Beldman I, Rivera A, Wheeldon I, Cutler SR
  TITLE     Synthesis of large single-transcript pathways from oligonucleotide 
            pools: Design of STARBURST, an autobioluminescent reporter.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Aug 5;122(31):e2508109122. doi: 
            10.1073/pnas.2508109122. Epub 2025 Jul 29.
  PUBMED    40729380
REFERENCE   2  (bases 1 to 12622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-08-5"; volume: "122(31):e2508109122. 
            doi"; pages: " 10.1073/pnas.2508109122. Epub 2025 Jul 29"
COMMENT     Sequence Label: pPlantPOP
FEATURES             Location/Qualifiers
     source          1..12622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    188..212
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(258..510)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(550..1344)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(661..680)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1271..1290
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1378..1557)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2457..2491
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2500..2511
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3191..3262
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3278..3305
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        4558..4902
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4846..4866
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4976..5653
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5156..5176)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5568..5591
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      5677..5929
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5964..5982)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6171..6195
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7496..8125
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8554..9627
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9693..9887
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10123..10145)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10231..10371
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10286..10305
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10386..10403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10557..11145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10637..10656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11233..12024)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
       61 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
      121 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
      181 tggctggtgg caggatatat tgtggtgtaa acataacgga tccggtctca ggagagcgat
      241 cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta
      301 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat
      361 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
      421 gtaattcaac agaaattata tgataatcat ggcaagaccg gcaacaggat tcaatcttaa
      481 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagagt ccgaacccca
      541 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gctatgcgct gcgaatcggg
      601 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
      661 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
      721 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcgtcgcc
      781 gtgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg cgaacagttc
      841 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
      901 catccgagta cgtgctcgct cgatgcggtg tttcgcttgg tggtcgaatg ggcaggtagc
      961 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     1021 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     1081 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     1141 cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt cggtcttgac
     1201 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     1261 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     1321 gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat gagactctaa
     1381 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     1441 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     1501 ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt gcggttctgt
     1561 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcatac cgtgactccc
     1621 ttaattctca tgtatctccg tcaggagtct caatggtaac tttactcttt atttaaccat
     1681 acattttttt ttattttttt cactttgttc ttcatccact attgttcttt gttcatcttg
     1741 aacaaaagct ccctccttct ttgttcttca tccaccattg ttcttcatca atcatttcgc
     1801 tgtcaggagg tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc cggcacacac
     1861 gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa taaggcaatt
     1921 agccaaaaac aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt attattagct
     1981 attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct
     2041 tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca atttctcaaa
     2101 atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt
     2161 attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata tgttctttgg
     2221 tttagattct gttaatctta gatcgaagac gattttctgg gtttgatcgt tagatatcat
     2281 cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa tttgagtttt
     2341 gtcgaataat tactcttcga tttgtgattt ctatctagat ctggtgttag tttctagttt
     2401 gtgcgatcga atttgtcgat taatctgagt ttttctgatt aacagaatga gagacgttta
     2461 cggctagctc agtcctaggt atagtgctag ctactagaga aagaggagaa atactaaatg
     2521 agtgtgatcg ctaaacaaat gacctacaag gtttatatgt caggcacggt caatggacac
     2581 tactttgagg tcgaaggcga tggaaaaggt aagccctacg agggggagca gacggtaaag
     2641 ctcactgtca ccaagggcgg acctctgcca tttgcttggg atattttatc accacagtgt
     2701 cagtacggaa gcataccatt caccaagtac cctgaggaca tccctgacta tgtaaagcag
     2761 tcattcccgg agggctatac atgggagagg atcatgaact ttgaagatgg tgcagtgtgt
     2821 actgtcagca atgattccag catccaaggc aactgtttca tctaccatgt caagttctct
     2881 ggtttgaact ttcctcccaa tggacctgtc atgcagaaga aaacacaggg ctgggaaccc
     2941 aacactgagc gtctgtttgc acgagatgga atgctgctag gaaacaactt tatggctctg
     3001 aagttagaag gaggcggtca ctatttgtgt gaatttaaaa ctacttacaa ggcaaagaag
     3061 cctgtgaaga tgccagggta tcactatgtt gaccgcaaac tggatgtaac caatcacaac
     3121 aaggattaca cttcggttga gcagtgtgaa atttccattg cacgcaaacc tgtggtcgcc
     3181 taataagctt caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     3241 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     3301 ctgcgcgtct ctgcttagct ttcgttcgta tcatcggttt cgacaacgtt cgtcaagttc
     3361 aatgcatcag tttcattgcg cacacaccag aatcctactg agtttgagta ttatggcatt
     3421 gggaaaactg tttttcttgt accatttgtt gtgcttgtaa tttactgtgt tttttattcg
     3481 gttttcgcta tcgaactgtg aaatggaaat ggatggagaa gagttaatga atgatatggt
     3541 ccttttgttc attctcaaat taatattatt tgttttttct cttatttgtt gtgtgttgaa
     3601 tttgaaatta taagagatat gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg
     3661 cctctaatga ccgaagttaa tatgaggagt aaaacacttg tagttgtacc attatgctta
     3721 ttcactaggc aacaaatata ttttcagacc tagaaaagct gcaaatgtta ctgaatacaa
     3781 gtatgtcctc ttgtgtttta gacatttatg aactttcctt tatgtaattt tccagaatcc
     3841 ttgtcagatt ctaatcattg ctttataatt atagttatac tcatggattt gtagttgagt
     3901 atgaaaatat tttttaatgc attttatgac ttgccgctgt caggagacta gagccaagct
     3961 gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag
     4021 aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct
     4081 cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct
     4141 gatcaagacg atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt
     4201 taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt
     4261 tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag
     4321 gcaagtaata gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga
     4381 gtcaaaaatt cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat
     4441 acagagtctt ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga
     4501 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     4561 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     4621 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     4681 taaaggaaag gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc
     4741 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     4801 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     4861 cccttcctct atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca
     4921 attaccacaa caaaacaaac aacaaacaac attacaattt actattctag tcgaaatggc
     4981 ctcctccgag aacgtcatca ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt
     5041 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggccacaa
     5101 caccgtgaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     5161 cccccagttc cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta
     5221 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     5281 cggcgtggcg accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt
     5341 gaagttcatc ggcgtgaact tcccctccga cggccccgtg atgcagaaga agacgatggg
     5401 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagacaca
     5461 caaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat
     5521 ggccaagaag cccgtgcagc tgcccggcta ctactacgtg gacgccaagc tggacatcac
     5581 ctcccacaac gaggactaca ccatcgtgga gcagtacgag cgcaccgagg gccgccacca
     5641 cctgttcctg taggcttgga atggatcttc gatcccgatc gttcaaacat ttggcaataa
     5701 agtttcttaa gattgaatcc tgttgccggt cttgcgacga ttatcatata atttctgttg
     5761 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     5821 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     5881 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattgcca
     5941 agctaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6001 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6061 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6121 ataaatgctt caataatggg accgactcgc gctgtcatga gaccggatcc tgacaggata
     6181 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
     6241 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
     6301 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
     6361 tcagtgcagc cgtcatctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
     6421 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
     6481 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
     6541 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
     6601 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
     6661 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
     6721 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
     6781 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
     6841 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     6901 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
     6961 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     7021 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     7081 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     7141 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     7201 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     7261 tcgcggccgg gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg ctgcatgaaa
     7321 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
     7381 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
     7441 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
     7501 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
     7561 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
     7621 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
     7681 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
     7741 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     7801 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     7861 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     7921 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     7981 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     8041 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     8101 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     8161 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     8221 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     8281 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     8341 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     8401 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     8461 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa
     8521 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
     8581 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
     8641 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
     8701 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
     8761 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
     8821 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
     8881 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
     8941 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
     9001 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
     9061 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
     9121 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
     9181 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
     9241 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
     9301 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
     9361 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
     9421 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
     9481 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
     9541 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
     9601 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
     9661 gcaggggaaa aaggtcgaaa aggactcttt cctgtggata gcacgtacat tgggaaccca
     9721 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
     9781 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
     9841 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
     9901 cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
     9961 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
    10021 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
    10081 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    10141 cccggtgacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    10201 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    10261 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10321 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
    10381 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10441 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10501 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10561 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10621 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10681 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10741 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10861 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10921 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10981 tacggctaca ctagaagrac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11041 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11161 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11221 cattctaggt gattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
    11281 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
    11341 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
    11401 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
    11461 aagcactaca tttcgctcat caccagccca gtcgggcggc gagttccata gcgttaaggt
    11521 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
    11581 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
    11641 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
    11701 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
    11761 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
    11821 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
    11881 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
    11941 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
    12001 ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg
    12061 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
    12121 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
    12181 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
    12241 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
    12301 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc
    12361 atttctgtcc tggctggaac agaacttatt atttccttcc tcttttctac agtatttaaa
    12421 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    12481 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    12541 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
    12601 ttacaatcaa catgctaccc tc
//
