LOCUS       sequence-484633-        7792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse RBP2 (accession ID NP_001074857.1), CMV promoter-enhancer, 
            downstream WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Kapoor R, Schwenzer N, Dresbach T, Lehnart SE, Moser T
  TITLE     Establishing synthetic ribbon-type active zones in a heterologous 
            expression system
  JOURNAL   eLife13:RP98254
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "eLife13:RP98254"
COMMENT     Sequence Label: pAAV-CMV-RBP2
FEATURES             Location/Qualifiers
     source          1..7792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     enhancer        323..626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             4273..4302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    4326..4914
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4379..4399)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4797..4808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4947..4964)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4953..5160
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5281..5736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5368..5387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5578..5599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5803..5822)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5922..5944
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5982..6000)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6068..6172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6173..6241,6242..7033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6391..6410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7204..7792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7693..7712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcaggctg
      241 cagagaggga gtggccaact ccatcactag gggttcctca gatctaggat cacgcgttct
      301 agatcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcggccgggc ccagcacccc
      661 aaggcggcca acgccaaaac tctcccccct cctcttcctc aatctcgctc tcgctctttt
      721 tttttttcgc aaaaggaggg gagagggggt aaaaaaatgc tgcactgtgc ggcgaagccg
      781 gtgagtgagc ggcgcggggc caatcagcgt gcgccgttcc gaaagttgcc ttttatggct
      841 cgagcggccg gccgcggcgg cgccctataa aacccagcgg cgcgacgcgc caccaccgcc
      901 gagaccgcgt ccgccccgcg agcacagagc ctcgcctttg ccgatccgcc gcccgtccac
      961 acccgccgcc aggtaagccc ggccagccga ccggggcagg cggctcacgg cccggccgca
     1021 ggcggccgcg ccaccatgcg agaggctgct gagcggcggc aacagctgga gttggagcat
     1081 gagcaagccc tggccttcct caatgccaag cagcaggaga tccagctact gcagcaggct
     1141 caggttgaag ctaagaaaga gcatgaaggt gctgtgcagc tgttagagaa caccctggac
     1201 tgcatgcagt ccaaggtccg agagctggaa gagaaatgcc gtgtgcagag tgagcagttt
     1261 aacttgttgt cccgggacct ggaaaagttc cggcaacaca ctgggagtat tgacctgctg
     1321 ggcagcagct cagtggccct gctggatgtt cccttggccc ctggcaagcc tttccctcag
     1381 tacatgaatg gactagccac ctccatccac aaaggtcacg agggccccac tggacactac
     1441 tctgtgattg gtgactatat tccgctgtct ggggacaagc tggagtctcc gtgtgtgaag
     1501 ccctccttcc tcttgcgatc cagcagccca agatgcagat ttgagtccga gatggacaat
     1561 gaccggaact ctaacaactc caagcaaagc agctcgggga aggtgcacct gtgtgtggcc
     1621 cgctacagtt acaacccctt cgatgggccc aatgagaacc cagaagctga gctgcctctc
     1681 acggcgggaa agtacctcta tgtctatggg gacatggatg aagatgggtt ctatgaagga
     1741 gagcttctgg atgggcaaag gggcttggtg ccctccaact ttgtggattt tatccaggac
     1801 aatgagtcgc ggctggctgg tactctgggg agtgagcagg accagaactt tctcaaccac
     1861 tctggcatca gtctggagcg cgacagcatc cttcaccttc actccccaac tcaagtggac
     1921 tcggggatca ccgacaatgg tggggggacc ctggacgtga acatcgacga catcggagaa
     1981 gacaccgtgc cttaccctag gaaaatcacc cttatcaaac agcttgccaa aagtgtcatc
     2041 gtgggctggg agccccctgc tgtgcctccc ggctggggca ccgtgagcag ttataatgtg
     2101 ctggtggaca aagagacacg catgagcctt gccctgggta ggagaactaa ggcgctgatc
     2161 gagaaactta acacagctgc ctgcacctac cgcatctccg tgcagtgcgt cacgagccgg
     2221 ggaaactcag acgagctgca gtgcactctg ctggtgggca aggatgtggt ggtggcaccg
     2281 tcccagctgc gtgtggacaa catcacacag atctctgccc agctctcgtg gctgccgacc
     2341 aacagtaact acagccatat catcttcctc aacgaagaag aactggacat cgtgaaggca
     2401 gccaggtaca agtaccaatt cttcaacctc aggcctaata tggcctacaa ggtgaaggtc
     2461 ttggcccagc cacaccagat gccctggcag ctgccgctgg agcagagaga aaagaaggag
     2521 gcctgtgtgg agttctccac gctgcctgca ggacctccag ccccaccaca agatgtcact
     2581 gtccacgctg gggccacagc cgcctctgtt caggtctcct ggaagccccc tgcactgact
     2641 cccactgggt tgtccaatgg agcaaatgtc acaggatacg gcgtgtacgc caaagggcag
     2701 agggtggctg aggtcatcgc ccccacggca gatggcacgg cagtggagct gatccggctg
     2761 cgaagcctag aggccaaggc cgtgagtgtg cgtaccctgt ctgtacaggg agagtccatg
     2821 gattctgccc tcgctgccat cccccctgac ctcctggtgc ctccagcccc ccacccgagg
     2881 actgctcccc caccaaagcc attagcaagt gacatggata ccaaagacca acacctgggg
     2941 ccccacgtca aagtggatga gtcctgggag cagagccggt caccgggccc tgcacatggc
     3001 cacatgttgg aaccacctga catgcacagc gctggcccag gcagaaggtc accctcgccc
     3061 agccggatcc ttcctcagcc acaaggagcc cccgtgtcta ccactgtcgc caaggccatg
     3121 gcccgtgaag ctgcacagag ggtggctgag agcaacaggt tagagaaaag gagcctcttt
     3181 ctagagcaaa gcagtgcggg gcaatatacc aactcggacg aggaggacgg ctacgcctcc
     3241 cccgaggtca agaggagagg cacctcagtg gatgacttcc tcaaagggtc agagctgggc
     3301 aagcagcccc actgttgcca tggagatgag taccacacag agagcagccg ggggtcagac
     3361 ctgtcggaca tcatggagga ggatgaggag gagctatact cagagatgca gctggaggat
     3421 gggggccgcc gtcggcccag cggtacctct cacaacgccc tcaagatttt aggaaactcc
     3481 acgttgatgg gacgagcaga ccggatggaa cacgtgagcc gaaggtattc acacagtggc
     3541 ggagggtctc ataggcaccg cccagcgatg gctccatcca ttgatgaata caccgggcga
     3601 gaccatcttt ctccagactt ctatgatgag tccgaaactg accctggtgc tgaggagctc
     3661 ccagcccgta tctttgtggc tctgtttgac tatgacccac tgaccatgtc cccaaaccca
     3721 gacgctgctg aagaggagct tcccttcaaa gaaggacaga tcatcaaggt atatggagac
     3781 aaagacgcag atggcttcta ccgtggggag acctgtgcca ggctcggcct cattccctgt
     3841 aacatggtct ctgagatcca tgcggatgat gaggagatga tggatcagct gctgaggcaa
     3901 ggcttcctcc ctctgaacac gcctgtggag aaaatagaga gaagtagaag aagcggccgg
     3961 ggtcactctg tacccacacg aagaatggtg gctctctacg actatgatcc tagggaaagc
     4021 tctcctaacg tggatgttga ggctgaactt ccattttgca caggagacat tattactgtt
     4081 tttggtgaaa tcgatgaaga tggattttat tatggagagc tgaatgggca aaaaggcctc
     4141 gtgccttcca atttcctgga agaagtgcct gatgatgtgg aggtccacct ttctgatgct
     4201 ccgccccact actcccacga cccgcccatg cgctccaagg ccaaaaggaa gaagagtgtt
     4261 catttcacac ccgaacaaaa acttatttct gaagaagatc tgtaagcggc cgcaagctta
     4321 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4381 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4441 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4501 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4561 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4621 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4681 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4741 tgctcgcctg tgttgccacc tggattctgc gcgggacgcc cttctgctac gtcccttcgg
     4801 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4861 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4921 accgtcgact agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4981 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5041 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5101 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5161 gagatctgag gaacccctag tgatggagtt ggccactccc tctctgcagc ctggcgtaat
     5221 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5281 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5341 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5401 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5461 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5521 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5581 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5641 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5701 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     5761 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     5821 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5881 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5941 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     6001 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7201 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7261 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7321 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7381 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7441 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7501 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7561 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7621 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7681 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7741 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aa
//
