LOCUS       sequence-484646-       30498 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob 
            - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 30498)
  AUTHORS   Kreitz J, Yang V, Friedrich MJ, Pham J, Macrae RK, Zhang F
  TITLE     Targeted delivery of diverse biomolecules with engineered bacterial 
            nanosyringes.
  JOURNAL   Nat Biotechnol. 2025 Aug 12. doi: 10.1038/s41587-025-02774-x.
  PUBMED    40796978
REFERENCE   2  (bases 1 to 30498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Aug 12. doi: 10.1038/s41587-025-02774-x."
COMMENT     Sequence Label: pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9
FEATURES             Location/Qualifiers
     source          1..30498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(339..1154)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(339..1154)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1062..1081
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1146..1165
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1458..2606)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2878..3249)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            3282..3391
                     /note="incP origin of transfer"
     rep_origin      3544..4175
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4484..5072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4973..4992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13570..13593
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             13597..13617
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14209..14229
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14254..18357
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             14254..18357
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gagatgatga taagaatggt cagtcaccag ataaaatgat ctctggtcaa ttttaggaaa
       61 gctgaaaaaa cctgaccaaa tgcgtcgtaa attctatgct tagaaagact cggtgccagt
      121 gtaataccta agatatggtt tttatttcca tatctgttcg aaaacttaga aataacaagt
      181 tcaacaataa ccagggactt taaatatcgg atccttgtcg ggaagatgcg tgatctgatc
      241 cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta
      301 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      361 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      421 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      481 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      541 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      601 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      661 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      721 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      781 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      841 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      901 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      961 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1021 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1081 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     1141 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1201 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1261 ctttcgactt ccggcaagct atacgcgccc tagaattgtc aattttaatc ctctgtttat
     1321 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
     1381 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
     1441 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
     1501 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
     1561 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
     1621 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
     1681 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
     1741 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
     1801 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
     1861 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
     1921 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
     1981 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
     2041 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
     2101 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
     2161 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
     2221 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
     2281 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
     2341 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
     2401 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
     2461 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
     2521 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
     2581 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
     2641 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
     2701 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
     2761 ttccgaatct tgccctgcac gaataccagc tccgcgaagt cgctcttctt gatggagcgc
     2821 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
     2881 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
     2941 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
     3001 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
     3061 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
     3121 cagcaggtag gcctacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
     3181 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     3241 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     3301 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     3361 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     3421 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     3481 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgaccctt
     3541 tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc
     3601 aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg
     3661 atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg
     3721 ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac
     3781 gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca
     3841 gacgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac
     3901 tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg
     3961 ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg
     4021 tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt
     4081 tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg
     4141 gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg
     4201 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagccaag ctgacccgct
     4261 agggacgtga agtcgattcc ttcgatggtt agcaatcaaa gactcagagt gctgtctgga
     4321 gcgtgaatct aacggtacgt atctcgattg ctcggtcgct attcgcactc tgcgaaagtt
     4381 cgtaccgctc attcactagg ttgcgaatca tgaccaaaat cccttaacgt gagttttcgt
     4441 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4501 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4561 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4621 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4681 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggtgataagt
     4741 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4801 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4861 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4921 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4981 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5041 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5101 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5161 tggataaccg tattaccgcc tttgagtgag ctgtcgcgaa tgcatctaga tataattaca
     5221 tcttcatcat tcggtatttt aaatgacata tatcctcctt ccgaatagtt ttaatggtga
     5281 tgttcgattg gacagtgttt tccattatta aaattttaag tatcacatca tgtttaatga
     5341 aggattgatg tttattagaa tattcataat agctttgtct ttgcctctat gtaaatgaaa
     5401 ttgcataaaa agcaaattgc attattggca atgtacttat tctggatttt tcggcacgat
     5461 aaattattgg tgtaagtttt atatcatgag taaaaattcg gaatgatatt cctgtcattt
     5521 tgtggcggta gcgttactca agatattatc ctacatcata tgttgattat tgtaatcaat
     5581 cgagctattt tttgtctaaa aaggggcttt tatgtctaca agtacatctc aaattgcggt
     5641 tgaatatcct attcctgtct atcgctttat tgtttctgtc ggagatgaga aaattccatt
     5701 taatagtgtt tcaggattag atattagtta tgacaccatt gaataccgag atggtgttgg
     5761 taattggttc aaaatgccgg gtcagagtca gagcactaat atcaccttgc gtaaaggcgt
     5821 tttcccgggg aaaacagaac tgtttgattg gattaactct attcagctta atcaggtaga
     5881 gaaaaaggat attaccatca gtttaactaa tgatgcaggt accgaattat taatgacctg
     5941 gaatgtttct aatgcttttc ccacttcatt gacttcacct tcatttgatg ccaccagtaa
     6001 tgatattgca gtacaggaaa ttacgctgat ggcagatcgg gtgattatgc aggctgtttg
     6061 aagcattgat atttaatcat ctcatataag ggaactttta tgacaaccgt taccagttat
     6121 cctggcgttt atattgaaga attaaatagc ctggccttgt cagtttcaaa tagcgccaca
     6181 gcggttcctg tttttgctgt ggacgaacaa aaccaatata ttagtgaaga taatgcaatc
     6241 cgtattaatt cgtggatgga ttatcttaat ctgattggca attttaataa tgaagacaaa
     6301 ttagatgttt ctgtgcgtgc ttattttgcc aatggaggtg gatattgtta tctcgtcaaa
     6361 acaacgagtt tagaaaaaat tattccaacc ttggatgatg taaccttatt ggttgctgcg
     6421 ggcgaagata ttaaaacgac agtagatgtt ttatgtcagc caggaaaagg gttattcgca
     6481 gtctttgatg gccctgaaac agagttgact atcaacggtg cggaagaggc aaaacaagcc
     6541 tataccgcca caccattcgc tgcggtttat tatccttggt tgaaagcgga ttgggctaac
     6601 atagatattc cacccagtgc agtgatggcg ggagtttatg catcggtgga tttatcccgt
     6661 ggtgtatgga aagcgcctgc caatgttgcg ttgaaagggg gcctggaacc taaattttta
     6721 gtcacggatg aattgcaggg tgaatataac actggccgcg ctatcaatat gattcgtaat
     6781 ttcagtaaca caggtactac ggtttggggt gcaagaaccc tggaagataa agacaattgg
     6841 cgttatgttc cagtgcgacg cttgtttaat tctgtggagc gggatatcaa gcgtgccatg
     6901 agctttgcta tgttcgagcc taataatcag cctacttggg agcgggtacg ggcggcgatt
     6961 agcaactacc tttatagcct gtggcaacag gggggattag ctggcagcaa agaagaagac
     7021 gcttattttg tgcaaattgg taaaggtata acgatgacac aggagcagat tgatgcaggg
     7081 caaatgattg ttaaagtcgg tttggctgct gtacggcctg cggaatttat cattctccag
     7141 tttacgcaag atgtagaaca gcgttaatca tatgattatg aggagttatc atgtctgcta
     7201 ttctgaaagc gcctggcgtt tatattgaag aagacgcttc cctagcgttg tctgtcagta
     7261 acagcgcgac tgccgtgcct gtttttatcg gaaaatttac tccgacagtg gttgattcaa
     7321 tccaagtctg tacccgtatc agcaactggc ttgaattcac ttcctctttt tccctagctc
     7381 caacagttga gattgttgtc caatctaaca ctgaatctga atctgaatct gaaacttacc
     7441 actatattga gacaatcaat ctatctccag ctgtggaagc attgcgactc tattttcaaa
     7501 atggcggagg agcttgctat atctacccat taaatgatgc tgaagatgaa ttggttctgg
     7561 cggccatacc agaagtcatt gaacagaaag gtgatattac tctgttggtt tgcccggaac
     7621 tcgatctgga ttacaaaact aagatctatg gcgcagtgag ctcactgttg aatgataaca
     7681 aagtgggcta tttcctgatt gcggatagca atgatggaga atctgtgtca ggagtatgga
     7741 atagtgctaa ggccgccgcc tattatcccc agttggaaac taacctaaaa ttttccacgt
     7801 tgcctgggga taaggacatt cgtatcagcg gttatcagga tgatgatgaa acacataaac
     7861 cgaaaaactt ggatgagctc aggacaatca acgaggcgtt ggcacaggat attgatgcaa
     7921 gattgctcga ggagaaacaa cgtgctgtca tcattccgcc aagtgctgcc attgcgggca
     7981 tttattgcca aacggataat cgtcgcggtg tttggaaagc gccagccaac gttgcgctca
     8041 cagggatcgg gagtttgctt gataaggtag acgatgaacg gcagggagag atgaatgaca
     8101 agggaatcaa tgtcatccgt tcatttaccg accgtggttt tatggtctgg ggagcccgta
     8161 cttgtgtgga cgctgccaac atcagctggc gttatattcc tgttcgtcgc ctgttcaatt
     8221 ccgttgaacg agatatccgc caggcgctac gcgctgtgtt gtttgaaact aatagtcagc
     8281 ctacctgggt acgtgctaag gctgccgttg atcaatatct ttataccctt tggcagaaaa
     8341 atgcattgat gggtgctcgc ccggaagaag cttattttgt gcaaattggt caggatatca
     8401 ccatgtccga ggctgatatt aaacagggta agatgatcat gactgttggt ttggcagcag
     8461 tgcggccagc tgagttcatc attctgcaat ttacgcagga tgttgttcag taatctccat
     8521 gactaaacgc caggcactgt attgacagtg cctactctaa ccatcttgga ggaggtgatg
     8581 atgatggaga gactccaacc gggtgtgact ttaacagaaa gtataatcac gatgggtcag
     8641 caagagatac ccagtgctgt gccggtgttt attggttaca ccgttcgtta tccggaacaa
     8701 tcggaagcat cagtccgtat cgacagtttg gccgagtata ccagcctgtt tggtgacgac
     8761 catgtgatga tgtttgctgt caggcactat tttgataatg gcgggcaaca ggcatttgtt
     8821 ttacccctga aggacaatat gccatcagtg gagatgacca cagctgaagc ggaaaatctg
     8881 atagccgcat tgcgctctgc tacggttagc gaagccattg gtgggcatag tcagattaca
     8941 ctgattttgg taccggatat ggctcggctt aatgacagtg atattgatga ctcctcaacc
     9001 caggtaagcc tgtggtccca aggctgggag gcgctgctgc aattgagtca ggttaggccc
     9061 aacctctttg tgctgttaga tgcgccggat aatgttgaac aggcgcagaa gtgtatgaca
     9121 acgctatcgt cagattatcg tcaatggggg gcagcatatt ggcctcgtct ggaaactacc
     9181 tatcagaaag aaatatctgg caaggacaat gaatctcagg gaattttcca ggggactgtt
     9241 ctgtcaccca cagccgcggt cgcagcggta attcaacgca cggataacga cgcgggtgtt
     9301 tggaaagcac cggccaatat tgccttatcc caggttattc gacctgttaa atcttatctt
     9361 cagggaagtg tactgtttaa cagcagcggc acttcgctca atgtgatccg cagtttccca
     9421 ggtaagggca tacgggtatg gggatgccgc actctggaaa acacggataa tacgcagtgg
     9481 cgctatctgc aaacacgtcg gctggtttcc tatgtaacag cgcatttgac ccaattggct
     9541 cgcatgtatg tcttcgagcc aaataatgaa cttacctgga tgaagttaaa aggacaaagt
     9601 tacaactggt tacggcaatt atggttgcag ggtggcttgt atggttcaca ggaggatgag
     9661 gcatttaaca ttctgttagg cgtaaacgag acgatgactg aggatgatgt tcgtgcagga
     9721 aaaatgatca tgaaagttga gttggctgtg ttgtttcctg ccgaatttat tgagatcagt
     9781 ttggtgttta atacccaaac agaggcgctg tcttaagaag gaaaaagtac gatgaacgat
     9841 tattacacac ccgtggtatc ccatcgtttt atggcgagtt ttatttttaa ccgcattccc
     9901 gatccgctgg atattcgttt tcagcgtatc tctggcctta gtcgggaact acaggtgact
     9961 cagtacagtg agggaggaga aaatgcccgt aataactatt tagctgagaa aatccaacac
    10021 ggtacgttga ctttggaacg gggcgtgatg acagtctcgc cattgacctg gatgtttgat
    10081 cgggtattga gtggtgaaaa aatcgcttat gccgatgtgg tggtgatgct actgaatgaa
    10141 aattcactgc cattgtccag ttggacgttg agcaatgcgc tgccggtacg ctggcaaacc
    10201 agcgactttg acgctaacag caatgccata ttggtgaata cccttgaatt gcgttaccag
    10261 gatatgcgct ggcttggagt caaaatatga cagtagaaat cagagagtta cttatccagg
    10321 caaaggtagt gccatcaaca cgaccgactg aatcagaacg gcaaaaccat tctttgatac
    10381 aggaaagtct ggatgaggcg acttgggtgg aaacgataaa acgcgaagtg ttggccgcat
    10441 tacgcgatga ggaagggtgg cgtccatgag tctgattgaa cgtggtttag ctaagctgac
    10501 aattaatgct tataaggata gggaagggaa gatacgggca ggaacgttgc aggccatgta
    10561 taaccctgac tccttgcaac tggattacca aacggattat cagcaatccc aagcgattaa
    10621 tagcgaaaag caaagtagca tttatgtaca ggccaagccc gcagggttat cacttgaatt
    10681 aatttttgat gccacgatgc cgggtaacaa aacccccatt gaagagcagc tcatgcagct
    10741 caagcaactg tgcagtgtgg atgcaaccag taacgagacg cgattcctgc aagttaaatg
    10801 gggcaaaatg cgttgggaaa gtcggggtta ctttgctggc agggccaaga gtttgtctgt
    10861 gaattacact ttgtttgatc gtgatgcgac tcccttgagg gtacgggtaa tattggcatt
    10921 agtggctgat gaaagtctgg tgttgcagga gactgaacaa aatctgcaat ctccggcaaa
    10981 aatcgcatta cgcatacagg atggggtatc tctggctctg atggcagcca gtacggcatc
    11041 aacattgtca ggcggtgtgg attatctgac gctggcctgg caaaacggtc tggataatct
    11101 caatgggttc gttccgggtg aaatattgca ggccaccagg ggagacgaat catgagccac
    11161 caactgaaaa ttattgcaga tggtaaggca ctgtcacttt tggccgcggt agatgtggac
    11221 acctgttatc gggttaacag tataccttct gcgacattga aactgagcgt accggatagg
    11281 ccactctctt ctttcagtca gacggatgtt cagacagaac tggcccactg tcaggtaggg
    11341 aaaaccctgc gtctggaatt gattgatggt agcaaaaaat gggtgctgtt taatggtctt
    11401 attacccgta aggctctgag aattaagaat aagcaattat tgctcactct ggttgtcaag
    11461 catcggttgc aactgatggt ggatacccag cattcacagc tgtttaaaga caaaagcgaa
    11521 aaagcgatct taagcacgct attgaatcag accggaatca atgctcgctt cggaaagata
    11581 gcggcgttag atcaaaagca tgaacagatg gtgcaatttc gttgttcaga ctggcatttt
    11641 ctgttgtgcc gactgtcggc aaccggtgca tggttgttac ctgccataga agacgttcag
    11701 tttgttcaac ctgatgctct gaaatcaaac tcagcctata ccttgaagag caggggggat
    11761 gagaacaaag acatcgttgt caaggatgct tactggcagt ttgacaatca aatcaacccc
    11821 gctttgctgg aagtcagtgg ctgggatatc agtaagcagc aggtacaatc aggcggtcgc
    11881 tacggaaaaa tcgcgttggg taaggcggca ctctctcctg atggattggc atcccttaat
    11941 aaaacgggtt gggacatttg ttatagcagt ccgttaacaa cccaggaaag cggttatctg
    12001 gcacagggat tattgcttaa ccagcgcatt tctggggtga caggagaatt tttgctcaaa
    12061 ggagatgggc gttaccagtt gggagacaac attcagctga ctggatttgg ttcacagtta
    12121 gatggtacgg caagcattac tgaggttcgc caccgtctta atcggcgaat tgattgggaa
    12181 accacggtga gcattggttt acaacatgaa tatttgccga tattacctga tgctcccgaa
    12241 ctacatattg cgacagtagc gaaatatcag caggacagtg cggtgttaaa ccgtatcccc
    12301 attattctgc cggtactgaa tcgtcccaat gaatttttgt gggccagatt ggggaaacct
    12361 tatgctagcc atgaaagcgg tttctgtttt tacccagagc caggtgacga agttattatt
    12421 ggtttttttg aaaatgatcc gcgttatcca gttattttag gtgctatgca taatccgaaa
    12481 aataaggccc cttttgaacc aacccaagat aatagggaaa aagtattgat cgttaaaaaa
    12541 ggtgaagcgc aacaacaatt agtcattgat ggcaaagaga aaatgatccg aattaatgcg
    12601 ggtgaaaatc aaataatgct tcagcaagat aaagacattt ctctgtcaac gaaaaaagaa
    12661 ttaacactga aagcgcagac aatgaatgcc acgatggata aatcattggc aatgtccggg
    12721 aaaaacagtg ttgaaatcaa aggcgcaaaa attaatctta cccaatgaaa ggtgacgatg
    12781 aatggaaaat caaatactga cacaactcta tggtcgtggt tgggcttttc ctccggtctt
    12841 ttcccttgaa aagggggtag agatggctga aggggcggaa gatgtgagac aaagtttgca
    12901 gattctgttt agtactgagc cgggggaacg tcttatgcgt gaaaattatg gctgcggatt
    12961 aaatgatttt atgtttgaaa atatccgcaa tgaacttatt gctgaaattg aatcccatat
    13021 ccatgacaac gtattacgat atgaaccccg ggctgatatg actgatattc aggttcgtca
    13081 atcccctggc atggggaata ctttgcaagt gcaggtcatg tatcgcctga gagggagtga
    13141 tatcaatcaa caaatccagg gagtacttgc actgagtgaa ggccgggtga cggaggtagt
    13201 atgagtagcc cgagcaaaaa gaatggtcgt agcggtccgc agccgcataa acgttgggtt
    13261 tttaccctga ataatccgag cgaagatgaa cgtaaaaaga ttcgtgatct gccgattagc
    13321 ctgttcgatt atttcattgt tggtgaagaa ggcaacgaag aaggtcgtac accgcatctg
    13381 cagggttttg caaattttgt taaaaagcag acctttaaca aagtgaaatg gtatctgggt
    13441 gcccgttgcc atattgaaaa agcaaaaggc accgatcagc agaacaaaga atattgtagc
    13501 aaagaaggta acctgctgat ggaatgtggt gcaccgcgta gccagggtca gcgtggtggc
    13561 agtggtggtg attataagga cgatgacgat aaggatccca agaaaaagcg caaggtaggt
    13621 ggttcaggtg gtggtggtag cggtggtggc ggttctggcg gtggcggtag tggcggaggt
    13681 ggaagcggag gcatgagtga agcgattgtg gtggatggtg acgtgttaca gtttgatccc
    13741 aactttggca atcggcaggt gacggttccc agcccaggaa aaattagcgg cacaggacat
    13801 gcgcaggtaa gtggaaaaaa agtgtgtatt ctgggggatg agaaacaggt cagggtttct
    13861 gcaacctata ttacaacaac acatactacg ccgggaacag gaaccattac tatcagtgct
    13921 ctggatgctg gccagcaggc ccttcagtgt accagtgggg cggctttaat tatcaagggg
    13981 cagcaattta cggcgatgtt tacgcctgaa ttgccagcca tgaataatac agtgactccg
    14041 ccacaaccgg atgttacgac accttcatca ggaaaaggac gttttatcac tcaacaaaat
    14101 tttgctaccg taaatggtgg ttcaggtggt ggtggtagcg gtggtggcgg ttctggcggt
    14161 ggcggtagtg gcggaggtgg aagcggaggc ggtggaagtg gcggtgcacc gaaaaagaaa
    14221 cgcaaagttg gtattcatgg tgttccggca gcaatggata agaaatactc aataggctta
    14281 gatatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct
    14341 aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg
    14401 gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt
    14461 agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag
    14521 atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa
    14581 gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat
    14641 gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg
    14701 gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg
    14761 attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta
    14821 caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa
    14881 gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc
    14941 cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc
    15001 cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat
    15061 acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg
    15121 tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat
    15181 actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat
    15241 caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa
    15301 atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa
    15361 gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta
    15421 ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct
    15481 attccccatc aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt
    15541 tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct
    15601 tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct
    15661 gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa
    15721 tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca
    15781 aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat
    15841 gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt
    15901 gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc
    15961 aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct
    16021 tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat
    16081 gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg
    16141 gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa
    16201 cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt
    16261 attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc
    16321 aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa
    16381 aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt
    16441 agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa
    16501 gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca
    16561 actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa
    16621 gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa
    16681 aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat
    16741 attaatcgtt taagtgatta tgatgtcgat cacattgttc cacaaagttt ccttaaagac
    16801 gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac
    16861 gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc
    16921 aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt
    16981 gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag
    17041 catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt
    17101 attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat
    17161 ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta
    17221 aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc
    17281 tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata
    17341 ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa
    17401 attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact
    17461 ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg
    17521 ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca
    17581 attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa
    17641 aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg
    17701 gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg
    17761 gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa
    17821 gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt
    17881 cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca
    17941 agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca
    18001 gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt
    18061 attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa
    18121 gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt
    18181 attcatttat ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca
    18241 acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat
    18301 caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgacaaa
    18361 cgtccggcag caaccaaaaa ggcaggtcag gccaaaaaga aatagagtat tgactgaatt
    18421 aaatagaatt aacgaaggtg taaataatta tttatttgct gacgaatcgc tgtgacaaat
    18481 aaacacaggt gatgttatgg aattaaatga gttaactaac aaattgtcaa atttggtgcc
    18541 aatgaccgat tttaaattag ataatcgagc cagtttgcaa ttgcttaaat atattgaagc
    18601 gtatacgaag ataataccct ttaattctgg cgataaatat tggaatgact ttttctttat
    18661 gtcaggaaat acgccagaga aacttgcaaa attatatcag aaagaaatag aacccaatgg
    18721 ggagttatta cctcagcagg cttttttgtt ggcggttttg cgtttattgg aaacaccaat
    18781 atccttatta aatgtattac ctgctgctca tcgtgagctc tattatcggg agcttttagg
    18841 cttgtcttcc catgcggcac agcctgatca ggttgcttta tctatggaac tgaattcgac
    18901 agtgatggaa cagctgctcc ctgaaggaac cctgtttgag gctggtcagg atgaacaagg
    18961 caatgcattg caatatgccc tggatgccag tttgctggct aatcgtggat atatcagtga
    19021 cttgcgctgg ttacggaatg acggggaaaa gcaatgggtt acttctgctc catgggattt
    19081 acaggcacag gtgtcactgc cgtctgatgg gatacgatta tttggtaaga caaatagtga
    19141 tcagcaggta tttggtgggg tgttgataac gtcatcactt ctggcgatgg aagcggggat
    19201 aaggaagatc attgttactt ttgagcagga gatgaacacc caagaactgg tggcacaggt
    19261 cagcagtgga aatcaatggc taacattgac gtctgaggta aataagaaag aggtcacact
    19321 gacactgtca gacaaagaac cggcaatcag tgcgccagag gatctggata atctcttttt
    19381 cacgcaaccg gtactcaggc tacagggaaa ggatagtcag gcactgccgg aggtgacggg
    19441 tatcagcgtt tcggaaaagg atgatactaa ggatacctct tttgagatgt atcacttaac
    19501 accatttggt tatagcagtg atatagagcc attggaggaa aatccagcgt tatatttagg
    19561 ctttactgat gtaaagccag ggcaaacact ggcgctgtat tggaaattaa aatccccgca
    19621 gcaaccaacc gtttcctggt attacctgga tcaacataat caatgggctg aattggattc
    19681 atgggtcagt gatggaaccc agaatctgta tcaggatggt acttggcacg ttgagttgcc
    19741 tgtggatgca tccaatcagg cagagcagat gccagttgga cgctattggt tgcgggcagt
    19801 ggtggaggta cccgctcatg agggggcgtt ggggaaggct ccttggctat atggtctaat
    19861 ctataacgcc atgacggcaa ccttggttaa tgtagatagc atcagtgaca gccatttctt
    19921 aacccctttg cctgccagca gcatacagcg gcccgttgaa cccatcattg tgttggcatc
    19981 ggtcaaccag ccttgggcat catggggtgg acgtatacct gaatcctaca gtgccttttt
    20041 tgaacggata gctcaaaacc tgtctcatcg aaaccggtcc ttaacctggg gaaatatggt
    20101 gacattactc aaagagcgtt atgtcagcat ctttgatgtt aagtatccag gtaatgatga
    20161 actcaccaga gtgccagcat tggagcagca gcaactaaca gtgattccag caaaccggta
    20221 caacgatagc gatgattctc tgcgtccggt actgaatcct gctcgtctgc aagagatggc
    20281 tgattggttg cagcagaaag actctccctg ggcctctatt gaggtcagga atccagaata
    20341 cttggatgtg aaaatccatt acgaggtgat ttttaaacct gatgtgaacg aagattttgg
    20401 ctatcgccag ctacagcagc aactgtgtga ggtgtatatg ccttggagca tagatgagca
    20461 gcggcccgtt gtattgaata acagcattaa ttatttccag ttgttagcca ctattcaaca
    20521 gcaaccgctg gttgagcgag tcactcgtct gacactacat cgggctgatt cttctgatga
    20581 gagtgatggt acagcatctg tggaagccaa agataatgaa gtgcttattt tagtctggga
    20641 agaggacgat aatctgcaat accgaggaaa tgactatgag taatcaggat gcactgtttc
    20701 atagcgttaa agacgatatt cactttgata ccttgctgga acaagctcat caggtgattg
    20761 aaaaacaggc tgaaaaactg tggagtgata cggcagagca tgatccgggt atcacatttt
    20821 tgcagggaat cagttacggt gtgtcagatt tggcttaccg acatacatta cccctgaaag
    20881 atttactgac tccggcgccg gatgagcagc agcaagaggg aatttttcct gccgaatttg
    20941 gcccgcataa tacactgact tgtgggccgg tgacagcgga tgattatcgc aaggcattgt
    21001 tagatctaca cagcagcgac agcctggatg gtactcagca ggatgagggg gattttctgt
    21061 tccggagtgt gcaactggtg cgtgaaccgg aaaaacagcg ttatacctat tggtatgatg
    21121 caaccaagag ggaatatagc tttgtcaaca gtgaaggggc taaagagttt accttgcggg
    21181 ggaattactg gttgtatctg gaaccaaccc gttggactca gggtaatatt gccgctgcta
    21241 ccagacaact gacagaattt ttgactaaaa atcgcaatat tggtgaatct gtcagcaaca
    21301 ttatctggct acaaccggtt gatctgccac tgttgctgga tgttgaactg gatgatgatg
    21361 taggtgcaca ggatgtcccc ggtatttttg cggcggtgta tagcaccgca gagcagtatc
    21421 tgatgcctgg agcacagcgt taccgtacgg aagtactgca aaatgctggg atgagcaatg
    21481 atcaaatctt cgaaggtcca ttattggaac atggctggat accagagctg ccggcagccc
    21541 gtgattatac tcaaaggctc actctcaatc ttagccggtt ggtaaatagt ctgcttgaga
    21601 ttgagggcat taaacatgtg aatcgtcttc gtctggatga tagcttcgat aaaactgcta
    21661 ttgaacccgt taagggggat acctggtcgt ggtcgatcaa agagggctat tatccacgtc
    21721 tttggggaga agacccactt aaccaattgg cgcaacaaaa tggcccgctt agggtgatag
    21781 ccaaaggagg gattagcgtc agtgtgagta aagagcaaat ccaggccagt ttacccagtc
    21841 aatcactgat tcaaaatgag ccggtaatat tggcttacgg ccagcaccgt gacgttggca
    21901 gctattatcc cgtcagtgat actttgccgc cttgctatgg actacaacat tctttgtctg
    21961 aaagtgaaca cttattgcca cttcatcaat ttatgttgcc atttgaacaa ttattggcct
    22021 gtggttgtca acagatagcc atgctcccgc ggttactggc ttttcagcgc gaaggttatg
    22081 aggtttgggg tgatcagtgg ccctttaagt caggctcagt gaatgatgac gcccatcaag
    22141 attatgcccc tgcattaaag gatttgttag gacagattgc gctggatagt gatcatgaat
    22201 tggatattat taattacttg ctgggttact ttggcacaca gcgggcaccg cgtaccttta
    22261 cgacacaact cgatgatttt cgtgcggtcc aacagggtta tctggcccag caaccgacat
    22321 tgacttacca ccgctccaat attcgtatcg atcaggtatc gtcgctacaa aaacgtattg
    22381 ctgctcgcat ggggctgggc ggtgagttgt ttaaacctca accggatctg agccaactgc
    22441 ctttttattt gattgaacat cgagcgttgc tgccagtcaa acccaatagt cagtttgata
    22501 aggaacagaa accagcctcg gtgacagagg aggggggcag ccaaacaggt caacattatg
    22561 tggtcattga acagaagggc attgatggca agctgacaca ggggcaagtg atcaatttaa
    22621 ttctgtatga aggagagcag ggagaaaccc aatttacgat acgcggtcag atggtattca
    22681 aaaccgaggg ggataagttt tggttggatg tgaataatag tgcgcaactg gaatataatc
    22741 tggcgcgggt aatgacagca gccaaggcga gtaaactctt ttggcaaaac agcccggtat
    22801 ggatggagga tatgggctat cgtctggcct atgctagtga ccaatcctca ttgcctgtga
    22861 atcaacggcg cttgacccgc acagtgcaaa ctccattccc gccgatggtt gttgtaggta
    22921 gcgaaatcac cctgttaaag caggtgggga tagtcaattt aaaaaaagcg gagtcagaaa
    22981 aactttatgc aaaagttgtt agctttgatc gcattgaagg gaccttgatt attgagcgtt
    23041 tgggtaattc cactctggct tttcctacct cggaagaggc gtggcggtat agttggtatt
    23101 tttcggggga gaaatatgaa aggactgacc gcttttcatt tgtgattagc gtagtagtga
    23161 acagtgactt aattaaattg cccggtgttg atccctataa attggaagaa tgggtgaaag
    23221 aaacgattct taccgaattt ccagctcata tttctatgat tatccattgg atggatcggg
    23281 aagccttttt aaatttcgcc aatacctatc agcgttggca aaataatggt acgccactgg
    23341 gggatgcggc ttattccatt ctagaaagtt tgacacttgg taaattgcca tctgccttaa
    23401 aaggtgttgg cacaatgcgt attgccacat ctagtcaaag agaagaagtg gtgggtagta
    23461 atggtgatca atggaataca gatggaataa cccagaatga attattctat gttcctaaag
    23521 agagctagga aaaataaata tctgccacta atgatgttga attaaatatg ttttctggag
    23581 ttaatcatga acgaaactcg ttataatgca actgtacaag aacaacaaac attatctaat
    23641 ccaaaagctg ttggacctga catcgataaa ttaaaggata aatttaaaga gggcagtatt
    23701 cccctgcaaa ccgatttcaa tgagttaatt gatattgccg atattggacg taaagcctgt
    23761 ggtcaagcgc cacaacaaaa tggcccagga gaaggattga aattggctga tgacggtacg
    23821 cttaatttaa aaataggcac tttttccaat aaagactttt ctccattaat attaaaagat
    23881 gatgttttat ctgtagatct tggtagtggt ctgactaatg aaaccaatgg aatctgtgtc
    23941 ggtcagggcg atggtattac agttaacact agcaatgtag ctgtaaaaca aggtaacgga
    24001 attagcgtta ctagtagtgg tggtgttgcc gttaaagtta gtgctaataa gggacttagc
    24061 gttgatagta gtggtgttgc agttaaagtt aatactgata agggaattag cgttgatggt
    24121 aatggtgttg cagttaaagt taatactagt aaaggaatta gcgttgataa tacaggtgtt
    24181 gcagttatag ctaatgctag taagggaatt agcgttgatg gtagtggtgt tgcagttata
    24241 gctaatacta gtaaaggaat tagcgttgat ggtagtggtg ttgcagttat agctaatact
    24301 agtaaaggaa ttagcgttga taatacaggt gttgcagtta tagctaatgc tagtaaggga
    24361 attagcgttg atggtagtgg tgttgcagtt atagctaata ctagtaaagg aattagcgtt
    24421 gatggtagtg gtgttgcagt tatagctaat actagtaaag gaattagcgt tgatagtagt
    24481 ggtgttgcag ttaaagttaa agctaatggc ggaattaaag tagatgctaa tggtgttgca
    24541 attgatccta ataatgtact ccccaaggga gtgattgtaa tgttctctgg cagtactgca
    24601 ccaactggtt gggcgttatg tgatggcaat aatggtacac caaatttaat cgatcgattt
    24661 attttaggtg ggaaagggac tgatattaat ggagtgagta ctaatacagc ttcaggtact
    24721 aaaaatagta agttattcga tttcagttct gatgaagcta cattaactat tgatggtaaa
    24781 acactgggga gaggcggttc tggcggcggt ggttctggcg gtaacgataa actgaccctg
    24841 tggaccacac cggcaccgag tccgaattgt cgtctgaatg cagaaaaaga tgcaaaactg
    24901 acactggttc tgaccaaatg tggtagccag attctggcaa ccgttagcgt tctggcagtt
    24961 aaaggtagcc tggcaccgat tagcggcacc gttcagagcg cacatctgat tattcgtttt
    25021 gatgaaaatg gcgtgctgct gaataatagc tttctggatc cggaatattg gaattttcgt
    25081 aatggtgatc tgaccgaagg caccgcatat accaatgcag ttggttttat gccgaatctg
    25141 agcgcatatc cgaaaagcca tggtaaaacc gcaaaaagca atattgttag ccaggtgtat
    25201 ctgaacggcg ataaaacaaa accggttaca ctgaccatta cactgaatgg cacccaagaa
    25261 accggtgata ccacaccgag cgcctatagc atgagcttta gctgggattg gagcggtcat
    25321 aactatatca atgaaatttt tgcgaccagc agctacacct ttagctatat tgcacaagaa
    25381 ggtggctctg gtggtggtgg ttcaggtggt cacgatcatg atattaagat aacgggcaca
    25441 ggaaaacatt ctcacaaaaa caaagtaaca gtcccttatt atattctggc tttcatcata
    25501 aagctttaat atatatgaaa aattgaaaat ataaattatc cattaataat aaagaggata
    25561 ttagcatgac ttcggagcca aatctgttaa accggattac aattactatt gaagctaata
    25621 atcaacaagt agctagaaaa gtattgcatg gctccttgct taatcaagct aatataaata
    25681 aattatttaa ttcatacttt aatgaatatg aaattaatag gggtgtttat ttagaaacat
    25741 taatcctgaa tcttggtacg ataaatttcc atgattttaa ttcattgttt cctactctcc
    25801 taaaagctgc attgaataaa gaattcagtc aatatcagat aaacaaccat agggaagaaa
    25861 tgctatttaa tgagacaata tcaaatcaag ctactgataa gtcttacata tttggcgata
    25921 acaaattaat tgatgcagag aatttcattc actttttata tcaaaagcat tccacattaa
    25981 atctagtaga agcaatggga aataatggta ttgaaaaatt aacaaatcag ttaacacaaa
    26041 tagaaaataa atttgcgtta ttattggcaa aaagttgttt gtctgaggaa ggcttaaaac
    26101 gactcttggc tatcaaacaa cccgatttat taatcgctat caatcgcaga ttatctgaaa
    26161 gaataaatag accacaatat caggagaagc ttgtttcctg cggacaactg atatttagtg
    26221 ctctgggata tatacaacag tacaatatac aggaaattcc taaaccggat gaaaaagtta
    26281 ttgcacgcat aacaactgaa cttaataata atggtttgct taatacaata cctattatta
    26341 cactatttcg tcagagtggg attaacgatt catcactaaa tgattggcta aagaaaatct
    26401 ggcaggtgag atcaatttca cagttatgca gaaagtatct ttctgctaag gaataccaat
    26461 atctgtcaga acattttgtt tcaaagagcg tcgataaaaa tagatatgat gaagagcccg
    26521 taaatcagag catattatca aggttgaata ataattccat taaagaagga aataatcaca
    26581 gtcaactctg tactctcagt agactatatt ctgaacccgt tgtattacct gaacaaacca
    26641 ttctacgtca ggttagtaat acagtagatc agagcatatt atcaaggttg aataatgcct
    26701 ccattaaaga aggaaataac caaagtcaac ttcgcactct cagtagacta tattctgagc
    26761 ccgttgcatt acctgaacaa accattccac gtcaggttag taatacaggt atattaattc
    26821 tatggccaat gctacctaca ctatttaacc agcttggtct acttgagaaa aagaaattta
    26881 tccatcgtca ggcccagttt aatgccgttg attttcttga ttacctgatt tggggaaccg
    26941 aagatgtgaa agtggaacga aaggttttga ataatgttct atgtgggtta atggctgatg
    27001 aaattactga accaatgcct attgaaccag aaaaacaatg gataataatt caatggctgg
    27061 acgctattat ctcccaactt tctggctgga aaaagttaag tcgtaatgac gtccgtcaat
    27121 tatttctaca acgaccagga gaattactga tcaatgaaca ggaaattaaa atcacaatac
    27181 agcaacaacc atttgatgct ctgttaactg attggccgtg gccaatgaat atggcttgtt
    27241 ttagctggtt gagtcaacca ttaaccatta cgtggttata accattgacc acaatgactt
    27301 agtctgagta aaaaatatga atatatcgcc tgttttttat gattcattga atcaggataa
    27361 cgaccgtgat ctatcgtttt tatttagcga actggaacga atagatctcg ctcttcaaca
    27421 ccatttttat tgtgtagaaa gtcagcgaag tgagctcctg gatgagtttc tgctcactga
    27481 ggcggaagtg gtgaccaggc tggataagcc acttggtaaa cctcattgga taaatgatga
    27541 ttatctggcg atatcgcaaa agggcaatgt aagcctaatg gcagcgtcca gattaatgga
    27601 tctgatcgaa cgctttgaac tgactgattt tgagcgcgat gttttactat taggcttatt
    27661 gccccatttt gatagccgct attatcgact gttttcgctg attcaagggg gacaacaggg
    27721 tcgattacct tcttttgcgc tggcattgga actgttttgc cactcggcgc tggagaaaca
    27781 ggtacagcaa gcgagttttc tgcaccgggc acctttgatg ggttgccagc tattatccat
    27841 cgatactagt caaaaaacgc tggcctggct ccagactccc tttattactg acagcggggt
    27901 atatcacttt ttactggggc atcactacat tatgccggct ttagaacatt gtgctgagtg
    27961 gttaacaccg acagggattg gctgttatcc tgaaggatta aaacaagtac tgggtaacgt
    28021 attgttatct gacaacgata atattagacc gattgtctta ttacggggaa tggccggcag
    28081 tgccagagct tataccatta ctaatatgat ggcttcagaa gggaagcaaa cactgctggt
    28141 agatatatcc aaacttgctg atagcgatga aaaaaacatt attcttcaga taaagcatat
    28201 tttgcgggaa acccgcatgc atggagcatg tttattatta cggaattttt gcttgttagt
    28261 ggaacagaat aaacaactat tggactccct gtcagagtta ttgaatcaac ctgaattaag
    28321 aattgtttgc ctgattgagc cttattcccc attggtatgg ctgaaaaaga taccggtatt
    28381 actgattgag atgccacttt taacgcctgc ggaaaaagcc agattgttaa ttgccagctt
    28441 accggataat tgttccgagg atattgatac gataacttta agccagcgtt acacttttaa
    28501 cccagaaacc ctgccattga ttttgcaaga ggcccagctt tatcaacagc agcgagatcc
    28561 gctggatatc ttgcagcaat gcgatatacg ccaggcatta aatttgcgtg ctcaacaaaa
    28621 tttcggtcaa ttggcacagc ggattattcc taagcgctca ttaaaggatt tattggtatc
    28681 cgatgagatt gctcagcagt tacgggaaat actcatagca attaagtatc gggaacaggt
    28741 tctggcggga gggtttaaag ataaaattgc ctatggcact ggtatcagcg ccctgtttta
    28801 tggtgattca ggcactggaa aaaccatggc agcagaagtg attgctgacc acattggcgt
    28861 tgacttaata aaagtggatt tatctacagt agtgaataaa tacatcggtg aaacagaaaa
    28921 aaacttatcc cgtattttcg atttggcgga acaggatgca ggggtattat tctttgatga
    28981 agctgacgca ctgtttggta aacgcagtga aactaaagat tcccaggaca gacatgccaa
    29041 tattgaagtt tcttacttat tacagcgcct ggagaattac ccgggtctgg tcattttatc
    29101 caccaataat cgtggtcatt tagacagtgc ttttaatcgt cgttttactt tcattacccg
    29161 ttttacttac ccggatgaaa aaatccgtaa aaaaatgtgg caggaaattt ggcctagaaa
    29221 tataaaaata tcggaagata tcgattttaa cgaattagct caacgaacaa gcgtgactgg
    29281 cgcgaatatc cgcaatattg ctttattgtc ttcattcttt gcttcagagc aggggaatga
    29341 tgaagtcagt aatgaaaata ttgaaattgc attgaagcgt gaattagcta aagtcggacg
    29401 attaacattt taaaagttat cacaatgaaa gtattgaaat attaaataaa tttattacca
    29461 aaaagttatc acgatataat ttaagagagg ttttttatgt taaacacgca aactattatt
    29521 gatgtcaata aggcaatgga tgccatgctg cgcgcatatc tgaatcaaga tattgccatt
    29581 cgttttgatc tacctgaatt ggatactatg caatctgatg cgatggtaag tatctttctt
    29641 tatgacattc atgaagattt acagcttcgc tcggcagaat caagagggtt tgatgtttat
    29701 gccgggaggt tattgcctgg ttgggtaaat attaaatgta actatctgat tacctattgg
    29761 gaagcttcta agccagcgac tgatgccagc agtccggata gccaacctga taaccaggca
    29821 atacaagtga tgtcacaagt attaaatgcc ttgattaata atcgtcaatt ggcaggtatt
    29881 cctggtgctt atactcaggt tgtaccgcct aaagagagtt taaatagcct ggggaatttc
    29941 tggcaatcac tgggtaatcg cccacggctt tctctcaatt attcagtgac agtacctgtt
    30001 agcctaaacg atggtcagga tagcgcgact ccggttaccg cggtttcttc tacagtggaa
    30061 caaacggcat cgctcagtca agaagtggtt agtcatgctt tacgcgaatt actcattacg
    30121 gaattaggag gaggagagga taaccggttg gtactgagta aagttgaatt atccgcagtg
    30181 aaagagacga tgactcaaga cagtccggct cagatgatta tattgttgtc tgtttcaggc
    30241 attacacgac aggaatattt gaaggaaatt gataatatct ttgatcgttg ggtaaataat
    30301 gctgaagtta ttaccactat tgatgattgt gggattagaa ttgaaagtat aacgaaagat
    30361 aatcttgtag gaatttaaat ttttttaatt aaataaagga attaatattg atttgataga
    30421 gcatgtaatc aataacttct atgaaaatgt tgaaaaacaa tacacggaaa cacttaactt
    30481 acaattaaat tgttaaag
//
