LOCUS       sequence-484671-        6601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The nucleotides coding human GALNTL5 variant #1 and 3xHA tag 
            sequence were inserted under the CAG promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Noda T, Uriu R, Mashiko D, Shinohara H, Qu Y, Taira A, Matzuk RM, 
            Tahala D, Nakano M, Araki K, Yu Z, Zhang Y, Matzuk MM, Ikawa M
  TITLE     GALNTL5 binds GalNAc and is required for migration through the 
            uterotubal junction and sperm-zona pellucida binding.
  JOURNAL   Nat Commun. 2025 Sep 17;16(1):8264. doi: 10.1038/s41467-025-63805-4.
  PUBMED    40962834
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-09-17"; volume: "16(1):8264. doi"; pages: " 
            10.1038/s41467-025-63805-4"
COMMENT     Sequence Label: pCAG1.1-human GALNTL5-3xHA
FEATURES             Location/Qualifiers
     source          1..6601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1617..1639
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1699..1718
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3105..3194
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(3281..3300)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3346..3401
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3400..3419)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3756..3775)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3757..3775)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3785..3802)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3785..3801)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3794..3816)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3943..4398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4030..4049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4240..4261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4424..4528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4529..4597,4598..5389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4747..4766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5560..6148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6049..6068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6302..6319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6436..6457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6472..6477,6478..6495,6496..6502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6510..6526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6515..6537
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6534..6550
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6534..6550
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6569..6589
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6571..6589
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg ggggggggcg cgccccgggg gggggggggg
      541 gggggggggg gggggggggg ggcggggggg gggggggggg ggcgcgccgg gcgggggggg
      601 ggggcccgcg caatgccctt tttctggtcg ccggcggggg ccgcgctccg ccaccgacgc
      661 cgtcgggcgt gggcggtggg tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
      721 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
      781 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
      841 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
      901 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
      961 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1021 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     1081 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     1141 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     1201 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     1261 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     1321 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     1381 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     1441 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     1501 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     1561 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     1621 cggcttctgg cgtgtgaccg gcggctctag cctctgctaa ccatgttcat gccttcttct
     1681 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaat
     1741 ctagagccgc catgagaaat gccataattc aaggtttatt ctatgggtcc ttgacatttg
     1801 ggatctggac agctctgtta ttcatatatt tgcaccataa tcatgtgagc agctggcaga
     1861 agaaaagcca ggagcctctg tcagcttggt cccctggaaa aaaagtgcat cagcaaatta
     1921 tctatggctc agagcaaata ccaaaacctc atgtaatagt caaaaggact gatgaagata
     1981 aagcaaagtc tatgttaggt acagatttta accatacaaa cccagaactt cataaagaac
     2041 ttttaaaata tggatttaat gtgattatca gtagaagctt gggcatcgaa agagaagtgc
     2101 cagataccag gagtaaaatg tgtcttcaaa aacattaccc agcccgcctc ccgactgcca
     2161 gcattgtcat ttgcttctat aatgaagaat gtaatgcctt gtttcagacc atgtccagtg
     2221 tcatgaacct cacgccacac tattttcttg aagaaattat tttggtagat gacatgagca
     2281 aagttgatga tttgaaagaa aaactagact atcacctgga aacttttcgg ggaaaggtta
     2341 aaataataag aaacaaaaag agagaggggc tgattcgagc aaggctgatt ggagcttctc
     2401 atgcttcagg ggatgttctg gtgttcctgg acagccactg tgaggtgaac agagtatggc
     2461 tggagcccct gctgcatgcc attgccaagg accccaaaat ggtggtgtgc cccctgatag
     2521 atgtcattga tgatagaact ctggagtata agccctctcc tcttgtaagg ggaacttttg
     2581 attggaacct acaatttaaa tgggataatg ttttctctta tgagatggat ggaccagaag
     2641 gatctactaa accaatccgg tcacctgcaa tgtctggagg aatttttgct atacgtcggc
     2701 attattttaa tgaaattgga cagtatgaca aggatatgga tttttgggga agagaaaatt
     2761 tggaactttc actaaggatc tggatgtgtg gaggccaact ctttataatc ccctgctctc
     2821 gagtaggaca tatcagtaag aaacaaactg gaaaaccttc tacaatcatc agtgctatga
     2881 cacataacta cctaagactg gtgcacgttt ggctggatga atataaggag cagttttttc
     2941 ttcgaaagcc tggtctgaaa tatgtcacct acggaaatat tcgcgagcgt gttgagttaa
     3001 ggaaacgact gggttgcaag tcatttcagt ggtatttgga taatgtcttc ccagagttgg
     3061 aggcatctgt gaacagcctg gaattcgata tcgggggcgg gggctaccca tacgatgttc
     3121 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg
     3181 ttccagatta cgcttagctc gagactcctc aggtgcaggc tgcctatcag aaggtggtgg
     3241 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     3301 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     3361 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     3421 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     3481 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     3541 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     3601 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     3661 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     3721 gaagatccct cgacttaatt aaggtaccca attcgcccta tagtgagtcg tattacgcgc
     3781 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     3841 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     3901 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     3961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4021 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4081 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4141 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4201 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4261 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4321 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4381 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4441 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4501 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4561 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4621 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4681 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4741 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4801 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4861 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4921 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4981 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5041 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5101 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5161 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5221 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5281 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5341 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5401 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5461 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6181 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6241 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6301 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata tgcaaaccgc ctctccccgc
     6361 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6421 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6481 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     6541 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     6601 g
//
