LOCUS       sequence-484677-       10158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual sgRNA targeting CTRL expressing dAPEX-BFP targeting to ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Thayer JA, Petersen JD, Huang X, Gruel Budet LM, Hawrot J, Ramos DM,
            Sekine S, Li Y, Ward ME, Narendra DP
  TITLE     A unified mechanism for mitochondrial damage sensing in 
            PINK1-Parkin-mediated mitophagy.
  JOURNAL   EMBO J. 2025 Nov 20. doi: 10.1038/s44318-025-00604-z.
  PUBMED    41266657
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2025 Nov 20. doi: 10.1038/s44318-025-00604-z."
COMMENT     Sequence Label: pLenti_DualsgRNA_CTRL_ER-dAPEX-BFP2
FEATURES             Location/Qualifiers
     source          1..10158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             118..828
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    complement(863..896)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    952..1540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1005..1025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1423..1434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1543..1559)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1544..1560)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2069..2249
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(2311..2899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2391..2410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3070..3861,3862..3930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3693..3712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3931..4035)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4110..4129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4301..4680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4682..4880
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4835..4855
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4898..5078
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5125..5250
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5747..5980
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6165..6209
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6507..6624
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(6877..6910)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     6914..6934
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     7159..7179
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7330..7349
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        8221..9399
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8452..9390
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9347..9367
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      9406..9415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     9412..9471
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             join(9475..10158,1..63)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQKAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPEVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 gccttctttg ctgattacgc tgaggctcac caaaagcttt ccgagcttgg gtttgctgat
       61 gccggcggta gcagccgtag tagcagcagt ggtggtggtg gtagtggtgg cggtggcatg
      121 gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc
      181 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
      241 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
      301 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
      361 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
      421 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
      481 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
      541 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
      601 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca
      661 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
      721 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
      781 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaataa ggacgagctg
      841 taagaattcg tcgagggacc taataacttc gtatagcata cattatacga agttatacat
      901 gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct
      961 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     1021 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1081 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     1141 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     1201 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     1261 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     1321 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     1381 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     1441 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     1501 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     1561 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     1621 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     1681 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     1741 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     1801 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     1861 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     1921 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     1981 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     2041 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     2101 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     2161 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     2221 cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag
     2281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2821 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2881 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2941 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3001 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3061 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3121 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3181 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3241 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3301 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3361 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3421 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3481 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3541 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3601 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3661 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3721 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3781 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3841 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3901 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3961 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4021 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     4081 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4141 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     4201 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     4261 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     4321 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4381 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4441 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4501 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4561 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4621 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4681 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4741 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     4801 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     4861 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     4921 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4981 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5041 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     5101 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     5161 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     5221 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     5281 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     5341 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     5401 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     5461 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     5521 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     5581 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     5641 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     5701 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     5761 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     5821 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     5881 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     5941 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     6001 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     6061 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     6121 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     6181 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     6241 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     6301 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     6361 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     6421 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     6481 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     6541 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     6601 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     6661 ttagtaccgg gcccgctcta gagatccgac gcgccatctc taggcccgcg ccggccccct
     6721 cgcacggact tgtgggagaa gctcggctac tcccctgccc cggttaattt gcatataata
     6781 tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg ttctttttaa
     6841 tactagctac attttacatg ataggcttgg atttctataa cttcgtatag catacattat
     6901 acgaagttat aaacagcaca aaaggaaact caccctaact gtaaagtaat tgtgtgtttt
     6961 gagactataa gtatcccttg gagaaccacc ttgttgggcc gtgggcaaca ctgtatgttt
     7021 cagagctaag cacaagagtg catagcaagt tgaaataagg ctagtccgtt tacaacttga
     7081 aaaagtggca cccgagtcgg gtgctttttt tgctcgaatc tacactcagc tatggcgcgc
     7141 cccaaggtcg ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga
     7201 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     7261 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     7321 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     7381 tatatcttgt ggaaagccag aaacatggcg ttggcactta gcgcagcgtt taagagctaa
     7441 gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc
     7501 accgagtcgg tgcttttttt ctcgagtact aggatccatt aggcggccgc cgggaggtgg
     7561 tccctgcagt tacgccaatg ataacccccg ccagaaaaat cttagtagcc ttcccttttt
     7621 gttttccgtg ccccaactcg gcggattgac tcggcccctt ccggaaacac ccgaatcaac
     7681 ttctagtcaa attattgttc acgccgcaat gacccacccc tggcccgcgt ctgtggaact
     7741 gacccctggt gtacaggaga gttcgctgct gaaagtggtc ccaaaggggt actagttttt
     7801 aagctcccaa ctccccctcc cccagcgtct ggaggattcc acaccctcgc accgcagggg
     7861 cgaggaagtg ggcggagtcc ggttttggcg ccagccgctg aggctgccaa gcagaaaagc
     7921 caccgctgag gagactccgg tcactgtcct cgccccgcct cccccttccc tccccttggg
     7981 gaccaccggg cgccacgccg cgaacggtaa gtgccgcggt cgtcggcgcc tccgccctcc
     8041 ccctagggcc ccaattccca gcgggcgcgg cgcgcggccc ctccccccgc cgggcgcgcg
     8101 cccgctgccc cgcccttcgt ggccgcccgg cgtgggcggt gccacccctc cccccggcgg
     8161 ccccgcgcgc agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga
     8221 gatgcatgtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     8281 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     8341 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     8401 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     8461 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     8521 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     8581 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     8641 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     8701 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     8761 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     8821 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     8881 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     8941 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     9001 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     9061 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     9121 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     9181 tagttctcgc gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     9241 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     9301 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     9361 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag ctagcgccac catggagaca
     9421 gacacactcc tgctatgggt actgctgctc tgggttccag gttccactgg tgacggaaag
     9481 tcttacccaa ctgtgagtgc tgattaccag aaggccgttg agaaggcgaa gaagaagctc
     9541 agaggcttca tcgctgagaa gagatgcgct cctctaatgc tccgtttggc attccactct
     9601 gctggaacct ttgacaaggg cacgaagacc ggtggaccct tcggaaccat caagcaccct
     9661 gccgaactgg ctcacagcgc taacaacggt cttgacatcg ctgttaggct tttggagcca
     9721 ctcaaggcgg agttccctat tttgagctac gccgatttct accagttggc tggcgttgtt
     9781 gccgttgagg tcacgggtgg acctgaagtt ccattccacc ctggaagaga ggacaagcct
     9841 gagccaccac cagagggtcg cttgcccgat cccactaagg gttctgacca tttgagagat
     9901 gtgtttggca aagctatggg gcttactgac caagatatcg ttgctctatc tgggggtcac
     9961 actattggag ctgcacacaa ggagcgttct ggatttgagg gtccctggac ctctaatcct
    10021 cttattttcg acaactcata cttcacggag ttgttgagtg gtgagaagga aggtctcctt
    10081 cagctacctt ctgacaaggc tcttttgtct gaccctgtat tccgccctct cgttgataaa
    10141 tatgcagcgg acgaagat
//
