LOCUS       sequence-484977-        1386 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His6-SUMO-SPMc-SpyCatcher003-Ctag in bacterial cells for 
            calcium-induced NeissLock protein ligation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Lim SYT, Keeble AH, Howarth MR
  TITLE     Split NeissLock with Spy-Acceleration Arms Mammalian Proteins for 
            Anhydride-Mediated Cell Ligation.
  JOURNAL   ACS Chem Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515.
  PUBMED    40947984
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515."
COMMENT     Sequence Label: His6-SUMO-SPMc-SpyCatcher003-Ctag
FEATURES             Location/Qualifiers
     source          1..1386
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1009..1347
                     /codon_start=1
                     /product="improved SpyCatcher variant that reacts with 
                     SpyTag003 at a rate approaching the diffusion limit (Keeble
                     et al., 2019)"
                     /label=SpyCatcher003
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVNEDGQVTVDG
                     EATEGDAHT"
ORIGIN
        1 atgggtagct ggagccatca ccatcatcat cacgggagcg atagcgaagt gaaccaggaa
       61 gcgaaaccgg aagttaaacc ggaagtgaaa ccggaaaccc atattaatct gaaagttagc
      121 gacggcagca gcgaaatctt ttttaaaatt aaaaaaacca ccccgctgcg tcgcctgatg
      181 gaagcctttg cgaaacgtca gggtaaagaa atggatagcc tgcgctttct gtatgacggc
      241 atccgtattc aggccgatca gaccccggaa gacctggata tggaagacaa cgatattatt
      301 gaagcgcatc gcgaacagat cggtggtgcg ttattcgacc atcgcaacca aggaatccgt
      361 accgcgaccg gctgggtaag tgccgatgac ggcctgcttg tacgcgattt gaatggcaac
      421 ggaattattg acaatggagc agaacttttt ggtgacaata cgaagttagc tgacgggtca
      481 ttcgctaaac atggctatgc agcgcttgcc gagctggata gtaatggaga caacattatc
      541 aacgcggcag atgccgcctt tcagacactg cgcgtctggc aagatcttaa ccaagacggt
      601 atttcacaag ccaacgaatt acgcactctg gaagagttag gtattcaatc gttggatctt
      661 gcgtataaag acgtgaacaa gaatttaggg aacggcaaca ccttggctca acagggcagc
      721 tacacaaaga cagacggtac gacggccaag atgggagacc tgctgttggc cgctgataac
      781 ttgcacagcc gtttcaaaga taaagtcgaa cttaccgcag aacaggccaa ggccgcgaat
      841 cttgccggta tcggccgttt acgcgacctg cgtgaggctg ctgccctgtc tggggactta
      901 gcgaatatgt taaaggcata tagtgcggct gagacaaagg aagcccagtt agcgttgtta
      961 gacaatttaa tccataaatg ggcagagaca gacgggagcg gtgccatggt aaccacctta
     1021 tcaggtttat caggtgagca aggtccgtcc ggtgatatga caactgaaga agatagtgct
     1081 acccatatta aattctcaaa acgtgatgag gacggccgtg agttagctgg tgcaactatg
     1141 gagttgcgtg attcatctgg taaaactatt agtacatgga tttcagatgg acatgtgaag
     1201 gatttctacc tgtatccagg aaaatataca tttgtcgaaa ccgcagcacc agacggttat
     1261 gaggtagcaa ctccaattga atttacagtt aatgaggacg gtcaggttac tgtagatggt
     1321 gaagcaactg aaggtgacgc tcatactgga tccagtggta gcgggagcgg tgagccagag
     1381 gcttaa
//
