LOCUS       sequence-484978-        1416 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tPA-TGFa-SPMn-SpyTag003 in mammalian cells for 
            calcium-induced NeissLock protein ligation to epidermal growth 
            factor receptor (EGFR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Lim SYT, Keeble AH, Howarth MR
  TITLE     Split NeissLock with Spy-Acceleration Arms Mammalian Proteins for 
            Anhydride-Mediated Cell Ligation.
  JOURNAL   ACS Chem Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515.
  PUBMED    40947984
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515."
COMMENT     Sequence Label: tPA-TGFa-SPMn-SpyTag003
FEATURES             Location/Qualifiers
     source          1..1416
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1366..1413
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
ORIGIN
        1 atggacgcta tgaagcgagg tctgtgttgc gtgctgctgc tttgtggtgc ggtattcgta
       61 tccccatctt accagggcaa tagcgattgc tatttcggca atggctctgc ctatcggggc
      121 acccattcac tgaccgagtc cggggcttct tgccttccct ggaactccat gatactcatt
      181 gggaaagtct acacagcgca gaacccaagc gcgcaagcgc tcggccttgg gaagcacaat
      241 tattgcagaa accccgacgg cgacgctaaa ccatggtgcc atgtcctcaa gaatcgcagg
      301 cttacgtggg agtattgcga tgttccttct tgttccacat gtggtttgcg ccaatacagc
      361 caaccgcaat ttaggattaa gggcggcctt ttcgccgata tcgcatcaca cccgtggcaa
      421 gctgcaatct ttgccgctgc agccgctagt ccaggggagc gctttctttg tggcggtatc
      481 cttatatctt cctgctggat actcagcgcc gcccattgct tccaagaacg atttccaccg
      541 catcatctga ccgtcatact tggtaggacg tatagggtag tccccgggga ggaagaacaa
      601 aaattcgagg tagaaaaata catcgtacat aaggagttcg acgatgatac ttacgataac
      661 gatatagccc tgctccagct gaagagcgac agtagtagat gtgctcagga atcctctgta
      721 gttcggaccg tctgtcttcc gccggcggac cttcaacttc cggactggac cgagtgcgaa
      781 ttgagcggct acggtaagca cgaagcactc tccccatttt actccgagcg gcttaaggaa
      841 gctcacgtgc gactctaccc cagctcacgg tgcacatcac agcacttgct gaatcggact
      901 gttaccgaca atatgctctg tgcgggagat accaggtcag gtggacctca agctaacctt
      961 cacgatgcgt gccagggtga ttcaggtggt ccgcttgtct gtcttaacga cgggcgaatg
     1021 accctggttg ggatcatatc atgggggctg gggtgtgggc aaaaagatgt gccgggagta
     1081 tataccaagg ttacgaacta cctggattgg atcagagaca atatgcgccc cggcagcagc
     1141 gtcgtctccc attttaacga ttgccccgac agccacacac aattctgttt tcatgggacc
     1201 tgtcggttct tggtacagga ggataaacct gcttgcgtgt gccatagcgg ctacgtcggg
     1261 gcaagatgcg agcacgcgga tctgttggct ggcagctatg acccgctcgc cttggatctc
     1321 gatggtgatg gtatagagac agtagccgct aaagggggga gcgggcgggg cgtacctcac
     1381 attgtgatgg tcgacgccta caaacggtac aagtag
//
