LOCUS       sequence-484979-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Neo-2/15-TGFa-SPMn-SpyTag003 in mammalian cells for 
            calcium-induced NeissLock protein ligation to epidermal growth 
            factor receptor (EGFR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Lim SYT, Keeble AH, Howarth MR
  TITLE     Split NeissLock with Spy-Acceleration Arms Mammalian Proteins for 
            Anhydride-Mediated Cell Ligation.
  JOURNAL   ACS Chem Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515.
  PUBMED    40947984
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515."
COMMENT     Sequence Label: Neo-2/15-TGFa-SPMn-SpyTag003
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             619..666
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
ORIGIN
        1 atggacgcta tgaagcgagg tctgtgttgc gtgctgctgc tttgtggtgc ggtattcgta
       61 tccccatctt accagggctc tggcccgaaa aaaaaaatac agctgcacgc ggaacatgct
      121 ttgtatgatg cgttgatgat actgaacatt gttaagacga atagcccgcc ggctgaagag
      181 aagctggagg attatgcctt caactttgaa cttattcttg aagagatcgc acgcctcttc
      241 gaatctggtg accagaagga tgaggccgag aaggcaaaac ggatgaaaga gtggatgaag
      301 cgaatcaaaa ctactgcctc tgaagacgag caggaggaaa tggcgaatgc tatcattaca
      361 atcctgcagt cctggatttt ttccggcagc agcgtcgtct cccattttaa cgattgcccc
      421 gacagccaca cacaattctg ttttcatggg acctgtcggt tcttggtaca ggaggataaa
      481 cctgcttgcg tgtgccatag cggctacgtc ggggcaagat gcgagcacgc ggatctgttg
      541 gctggcagct atgacccgct cgccttggat ctcgatggtg atggtataga gacagtagcc
      601 gctaaagggg ggagcgggcg gggcgtacct cacattgtga tggtcgacgc ctacaaacgg
      661 tacaagtagt ag
//
