LOCUS       sequence-484984-        5634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient expresses IRF1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Chou WC, Chen WT, Shen CY
  TITLE     A common variant in 11q23.3 associated with hyperlipidemia is 
            mediated by the binding and regulation of GATA4.
  JOURNAL   NPJ Genom Med. 2022 Jan 19;7(1):4. doi: 10.1038/s41525-021-00279-5.
  PUBMED    35046404
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ Genom 
            Med."; date: "2022-01-19"; volume: "7(1):4. doi"; pages: " 
            10.1038/s41525-021-00279-5"
COMMENT     Sequence Label: pXJ-Myc-IRF1
FEATURES             Location/Qualifiers
     source          1..5634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        313..516
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     467..487
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     513..537
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          678..1250
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1258..1277
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1305..1323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1344..1373
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(2441..2459)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2733..2854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2770..2789)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2824..2843
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2882..2899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3053..3641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3133..3152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3812..4603,4604..4672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4435..4454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4673..4777)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4845..4863
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4901..4923)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5023..5042
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5109..5564)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5246..5267)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5458..5477
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagagtccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
       61 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      121 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      181 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      241 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      301 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      361 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      421 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      481 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      541 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      601 cgcggccggg aacggtgcat tggaacggac ccctaggctt ttgcaaaaag ctccggatcg
      661 atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg
      721 taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat
      781 gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt
      841 gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta
      901 gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact
      961 ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt
     1021 tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg
     1081 gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt
     1141 tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca
     1201 aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1261 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac gactcactat
     1321 agggcgaatt caccatggca gaggagcaaa agctcatttc tgaagaggac ttgttgcgga
     1381 aacatatggg atccatgccc atcactcgga tgcgcatgag accctggcta gagatgcaga
     1441 ttaattccaa ccaaatcccg gggctcatct ggattaataa agaggagatg atcttccaga
     1501 tcccatggaa gcatgctgcc aagcatggct gggacatcaa caaggatgcc tgtttgttcc
     1561 ggagctgggc cattcacaca ggccgataca aagcagggga aaaggagcca gatcccaaga
     1621 cgtggaaggc caactttcgc tgtgccatga actccctgcc agatatcgag gaggtgaaag
     1681 accagagcag gaacaagggc agctcagctg tgcgagtgta ccggatgctt ccacctctca
     1741 ccaagaacca gagaaaagaa agaaagtcga agtccagccg agatgctaag agcaaggcca
     1801 agaggaagtc atgtggggat tccagccctg ataccttctc tgatggactc agcagctcca
     1861 ctctgcctga tgaccacagc agctacacag ttccaggcta catgcaggac ttggaggtgg
     1921 agcaggccct gactccagca ctgtcgccat gtgctgtcag cagcactctc cccgactggc
     1981 acatcccagt ggaagttgtg ccggacagca ccagtgatct gtacaacttc caggtgtcac
     2041 ccatgccctc cacctctgaa gctacaacag atgaggatga ggaagggaaa ttacctgagg
     2101 acatcatgaa gctcttggag cagtcggagt ggcagccaac aaacgtggat gggaaggggt
     2161 acctactcaa tgaacctgga gtccagccca cctctgtcta tggagacttt agctgtaagg
     2221 aggagccaga aattgacagc ccaggggggg atattgggct gagtctacag cgtgtcttca
     2281 cagatctgaa gaacatggat gccacctggc tggacagcct gctgacccca gtccggttgc
     2341 cctccatcca ggccattccc tgtgcaccgt agcagggccc ctcgaggggg ggcccggtac
     2401 cttaattaat taaggtacca ggtaagtgta cccaattcgc cctatagtga gtcgtattac
     2461 aattcactcg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gagatccaat
     2521 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattcaca
     2581 gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc
     2641 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2701 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2761 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2821 gttgtggttt gtccaaactc atcaatgtat cttaacgctc gactctagac tcttccgctt
     2881 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2941 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3001 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3061 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3121 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3181 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3241 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3301 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3361 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3421 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3481 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3541 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3601 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3661 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3721 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3781 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3841 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3901 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3961 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4021 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4081 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4141 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4201 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4261 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4321 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4381 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4441 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4501 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4561 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4621 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4681 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4741 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4801 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4861 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4921 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4981 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     5041 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     5101 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     5161 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     5221 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     5281 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     5341 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     5401 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     5461 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     5521 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     5581 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccag
//
