LOCUS       sequence-485074-        9002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  M13 helper genes cloned into the pSEVA631 vector. This plasmid does 
            not carry an M13 packaging signal. (This plasmid is a gift of the 
            Jaramillo Lab, where it is called PAJ297.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Pujar A, Pathania A, Hopper C, Pandi A, Calderon CR, Fugger M, Nowak
            T, Kushwaha M
  TITLE     Phage-mediated intercellular CRISPRi for biocomputation in bacterial
            consortia.
  JOURNAL   Nucleic Acids Res. 2024 Dec 27:gkae1256. doi: 10.1093/nar/gkae1256.
  PUBMED    39727169
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Dec 27:gkae1256. doi: 10.1093/nar/gkae1256."
COMMENT     Sequence Label: pSEVA63_Helper
FEATURES             Location/Qualifiers
     source          1..9002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(840..889)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(893..1114)
                     /codon_start=1
                     /gene="VIII"
                     /product="pVIII"
                     /label=M13 gene VIII
                     /note="major coat protein"
                     /translation="MKKSLALKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI
                     GYAWAMVVVIVGATIGIKLFKKFTSKAS"
     CDS             complement(1111..1209)
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             complement(1206..1307)
                     /codon_start=1
                     /gene="VII"
                     /product="pVII"
                     /label=M13 gene VII
                     /note="minor coat protein"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             complement(1309..1572)
                     /codon_start=1
                     /gene="V"
                     /product="pV"
                     /label=M13 gene V
                     /note="replication"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             complement(1584..2816)
                     /codon_start=1
                     /gene="II"
                     /product="pII"
                     /label=M13 gene II
                     /note="replication"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     primer_bind     complement(2966..2988)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      3003..3089
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      3204..3975
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3976..4638
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     oriT            4649..4757
                     /note="incP origin of transfer"
     CDS             complement(4968..5501)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5690..5695,5696..5712,5713..5718))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     terminator      complement(5749..5843)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(5916..7196)
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     CDS             complement(7174..8220)
                     /codon_start=1
                     /gene="I"
                     /product="pI"
                     /label=M13 gene I
                     /note="phage assembly"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             complement(8222..8560)
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             complement(join(8563..9002,1..835))
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
ORIGIN
        1 cataatcaaa atcaccggaa ccagagccac caccggaacc gcctccctca gagccgccac
       61 cctcagaacc gccaccctca gagccaccac cctcagagcc gccaccagaa ccaccaccag
      121 agccgccgcc agcattgaca ggaggttgag gcaggtcaga cgattggcct tgatattcac
      181 aaacgaatgg atcctcatta aagccagaat ggaaagcgca gtctctgaat ttaccgttcc
      241 agtaagcgtc atacatggct tttgatgata caggagtgta ctggtaataa gttttaacgg
      301 ggtcagtgcc ttgagtaaca gtgcccgtat aaacagttaa tgccccctgc ctatttcgga
      361 acctattatt ctgaaacatg aaagtattaa gaggctgaga ctcctcaaga gaaggattag
      421 gattagcggg gttttgctca gtaccaggcg gataagtgcc gtcgagaggg ttgatataag
      481 tatagcccgg aataggtgta tcaccgtact caggaggttt agtaccgcca ccctcagaac
      541 cgccaccctc agaaccgcca ccctcagagc caccaccctc attttcaggg atagcaagcc
      601 caataggaac ccatgtaccg taacactgag tttcgtcacc agtacaaact acaacgcctg
      661 tagcattcca cagacagccc tcatagttag cgtaacgatc taaagttttg tcgtctttcc
      721 agacgttagt aaatgaattt tctgtatggg gttttgctaa acaactttca acagtttcag
      781 cggagtgaga atagaaagga acaactaaag gaattgcgaa taataatttt ttcacgttga
      841 aaatctccaa aaaaaaaagg ctccaaaagg agcctttaat tgtatcggtt tatcagcttg
      901 ctttcgaggt gaatttctta aacagcttga taccgatagt tgcgccgaca atgacaacaa
      961 ccatcgccca cgcataaccg atatattcgg tcgctgaggc ttgcagggag ttaaaggccg
     1021 cttttgcggg atcgtcaccc tcagcagcga aagacagcat cggaacgagg gtagcaacgg
     1081 ctacagaggc tttgagggct aaagactttt tcatgaggaa gtttccatta aacgggtaaa
     1141 atacgtaatg ccactacgaa ggcaccaacc taaaacgaaa gaggcgaaag aatacactaa
     1201 aacactcatc tttgaccccc agcgattata ccaagcgcga aacaaagtac aacggagatt
     1261 tgtatcatcg cctgataaat tgtgtcgaaa tccgcgacct gctccatgtt acttagccgg
     1321 aacgaggcgc agacggtcaa tcataaggga accgaactga ccaactttga aagaggacag
     1381 atgaacggtg tacagaccag gcgcataggc tggctgacct tcatcaagag taatcttgac
     1441 aagaaccgga tattcattac ccaaatcaac gtaacaaagc tgctcattca gtgaataagg
     1501 cttgccctga cgagaaacac cagaacgagt agtaaattgg gcttgagatg gtttaatttc
     1561 aactttaatc attgtgaatt accttatgcg attttaagaa ctggctcatt ataccagtca
     1621 ggacgttggg aagaaaaatc tacgttaata aaacgaacta acggaacaac attattacag
     1681 gtagaaagat tcatcagttg agatttagga ataccacatt caactaatgc agatacataa
     1741 cgccaaaagg aattacgagg catagtaaga gcaacactat cataaccctc gtttaccaga
     1801 cgacgataaa aaccaaaata gcgagaggct tttgcaaaag aagttttgcc agagggggta
     1861 atagtaaaat gtttagactg gatagcgtcc aatactgcgg aatcgtcata aatattcatt
     1921 gaatccccct caaatgcttt aaacagttca gaaaacgaga atgaccataa atcaaaaatc
     1981 aggtctttac cctgactatt atagtcagaa gcaaagcgga ttgcatcaaa aagattaaga
     2041 ggaagcccga aagacttcaa atatcgcgtt ttaattcgag cttcaaagcg aaccagaccg
     2101 gaagcaaact ccaacaggtc aggattagag agtaccttta attgctcctt ttgataagag
     2161 gtcatttttg cggatggctt agagcttaat tgctgaatct ggtgctgtag ctcaacatgt
     2221 tttaaatatg caactaaagt acggtgtctg gaagtttcat tccatgtaac agttgattcc
     2281 caattctgcg aacgagtaga tttagtttga ccattagata catttcgcaa atggtcaata
     2341 acctgtttag ctatattttc atttggggcg cgagctgaaa aggtggcatc aattctacta
     2401 atagtagtag cgttaacatc caataaatca tacaggcaag gcaaagaatt agcaaaatta
     2461 agcaataaag cctcagagca taaagctaaa tcggttgtac caaaaacatt atgaccctgt
     2521 aatacttttg cgggagaagc ctttatttca acgcaaggat aaaaattttt agaaccctca
     2581 tatattttaa atgcaatgcc tgagtaatgt gtaggtaaag attcaaaagg gtgagaaagg
     2641 ccggagacag tcaaatcacc atcaatatga tattcaaccg ttctagctga taaattcatg
     2701 ccggagaggg tagctatttt tgagaggtct acaaaggcta tcaggtcatt gcctgagagt
     2761 ctggagcaaa caagagaatc gatgaacggt aatcgtaaaa ctagcatgtc aatcatatgt
     2821 accccggttg ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa
     2881 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     2941 tttaaccaat aggcctaggc ggcctcctgt gtgaaattgt tatccgcttt aattaaaggc
     3001 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     3061 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacagctggg cgcgcccccc
     3121 ctacgggctt gctctccggg cttcgccctg cgcggtcgct gcgctccctt gccagcccgt
     3181 ggatatgtgg acgatggccg cgagcggcca ccggctggct cgcttcgctc ggcccgtgga
     3241 caaccctgct ggacaagctg atggacaggc tgcgcctgcc cacgagcttg accacaggga
     3301 ttgcccaccg gctacccagc cttcgaccac atacccaccg gctccaactg cgcggcctgc
     3361 ggccttgccc catcaatttt tttaattttc tctggggaaa agcctccggc ctgcggcctg
     3421 cgcgcttcgc ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg cgcagcgacc
     3481 gcgcagcggc ttggccttga cgcgcctgga acgacccaag cctatgcgag tgggggcagt
     3541 cgaagggcga agcccgcccg cctgcccccc gagcctcacg gcggcgagtg cgggggttcc
     3601 aagggggcag cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa caagccccgg
     3661 aggggccact ttttgccgga gggggagccg cgccgaaggc gtgggggaac cccgcagggg
     3721 tgcccttctt tgggcaccaa agaactagat atagggcgaa atgcgaaaga cttaaaaatc
     3781 aacaacttaa aaaagggggg tacgcaacag ctcattgcgg caccccccgc aatagctcat
     3841 tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt acgcaacgca taattgttgt
     3901 cgcgctgccg aaaagttgca gctgattgcg catggtgccg caaccgtgcg gcacccctac
     3961 cgcatggaga taagcatggc cacgcagtcc agagaaatcg gcattcaagc caagaacaag
     4021 cccggtcact gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg gcttattgcg
     4081 aggaaaccca cggcggcaat gctgctgcat cacctcgtgg cgcagatggg ccaccagaac
     4141 gccgtggtgg tcagccagaa gacactttcc aagctcatcg gacgttcttt gcggacggtc
     4201 caatacgcag tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa gctcaacggc
     4261 cccggcaccg tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca gccccgcgac
     4321 cagttgcgcc tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga ccaggacgaa
     4381 tcgctgttgg ggcatggcga cctgcgccgc atcccgaccc tgtatccggg cgagcagcaa
     4441 ctaccgaccg gccccggcga ggagccgccc agccagcccg gcattccggg catggaacca
     4501 gacctgccag ccttgaccga aacggaggaa tgggaacggc gcgggcagca gcgcctgccg
     4561 atgcccgatg agccgtgttt tctggacgat ggcgagccgt tggagccgcc gacacgggta
     4621 acgctgccgc gccggtaggg ccggcctacg gccagcctcg cagagcagga ttcccgttga
     4681 gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta
     4741 cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc
     4801 tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa
     4861 tgaccccgaa gcagggttat gcagcggaaa aggacaacgc gcggaccgtt gtccaattta
     4921 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
     4981 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
     5041 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
     5101 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctctccg
     5161 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg
     5221 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
     5281 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
     5341 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
     5401 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
     5461 cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat
     5521 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca taggctgtac
     5581 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     5641 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
     5701 cgaacaggct tatgtcaaat ttaaatcgta attattgggg acccctggat tctcaccaat
     5761 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     5821 tactggatct atcaacagga gtccaagact agtcgccagg gttttcccag tcacgacgcg
     5881 gccgcaagct tgcatgcgcc gcgcttaatg cgccgctaca gggcgcgtac tatggttgct
     5941 ttgacgagca cgtataacgt gctttcctcg ttagaatcag agcgggagct aaacaggagg
     6001 ccgattaaag ggattttaga caggaacggt acgccagaat cctgagaagt gtttttataa
     6061 tcagtgaggc caccgagtaa aagagtctgt ccatcacgca aattaaccgt tgtagcaata
     6121 cttctttgat tagtaataac atcacttgcc tgagtagaag aactcaaact atcggccttg
     6181 ctggtaatat ccagaacaat attaccgcca gccattgcaa caggaaaaac gctcatggaa
     6241 atacctacat tttgacgctc aatcgtctga aatggattat ttacattggc agattcacca
     6301 gtcacacgac cagtaataaa agggacattc tggccaacag agatagaacc cttctgacct
     6361 gaaagcgtaa gaatacgtgg cacagacaat atttttgaat ggctattagt ctttaatgcg
     6421 cgaactgata gccctaaaac atcgccatta aaaataccga acgaaccacc agcagaagat
     6481 aaaacagagg tgaggcggtc agtattaaca ccgcctgcaa cagtgccacg ctgagagcca
     6541 gcagcaaatg aaaaatctaa agcatcacct tgctgaacct caaatatcaa accctcaatc
     6601 aatatctggt cagttggcaa atcaacagtt gaaaggaatt gaggaaggtt atctaaaata
     6661 tctttaggag cactaacaac taatagatta gagccgtcaa tagataatac atttgaggat
     6721 ttagaagtat tagactttac aaacaattcg acaactcgta ttaaatcctt tgcccgaacg
     6781 ttattaattt taaaagtttg agtaacatta tcattttgcg gaacaaagaa accaccagaa
     6841 ggagcggaat tatcatcata ttcctgatta tcagatgatg gcaattcatc aatataatcc
     6901 tgattgtttg gattatactt ctgaataatg gaagggttag aacctaccat atcaaaatta
     6961 tttgcacgta aaacagaaat aaagaaattg cgtagatttt caggtttaac gtcagatgaa
     7021 tatacagtaa cagtaccttt tacatcggga gaaacaataa cggattcgcc tgattgcttt
     7081 gaataccaag ttacaaaatc gcgcagaggc gaattattca tttcaattac ctgagcaaaa
     7141 gaagatgatg aaacaaacat caagaaaaca aaattaatta catttaacaa tttcatttga
     7201 attacctttt ttaatggaaa cagtacataa atcaatatat gtgagtgaat aaccttgctt
     7261 ctgtaaatcg tcgctattaa ttaattttcc cttagaatcc ttgaaaacat agcgatagct
     7321 tagattaaga cgctgagaag agtcaatagt gaatttatca aaatcatagg tctgagagac
     7381 taccttttta acctccggct taggttgggt tatataacta tatgtaaatg ctgatgcaaa
     7441 tccaatcgca agacaaagaa cgcgagaaaa ctttttcaaa tatattttag ttaatttcat
     7501 cttctgacct aaatttaatg gtttgaaata ccgaccgtgt gataaataag gcgttaaata
     7561 agaataaaca ccggaatcat aattactaga aaaagcctgt ttagtatcat atgcgttata
     7621 caaattctta ccagtataaa gccaacgctc aacagtaggg cttaattgag aatcgccata
     7681 tttaacaacg ccaacatgta atttaggcag aggcattttc gagccagtaa taagagaata
     7741 taaagtaccg acaaaaggta aagtaattct gtccagacga cgacaataaa caacatgttc
     7801 agctaatgca gaacgcgcct gtttatcaac aatagataag tcctgaacaa gaaaaataat
     7861 atcccatcct aatttacgag catgtagaaa ccaatcaata atcggctgtc tttccttatc
     7921 attccaagaa cgggtattaa accaagtacc gcactcatcg agaacaagca agccgttttt
     7981 attttcatcg taggaatcat taccgcgccc aatagcaagc aaatcagata tagaaggctt
     8041 atccggtatt ctaagaacgc gaggcgtttt agcgaacctc ccgacttgcg ggaggttttg
     8101 aagccttaaa tcaagattag ttgctatttt gcacccagct acaattttat cctgaatctt
     8161 accaacgcta acgagcgtct ttccagagcc taatttgcca gttacaaaat aaacagccat
     8221 attatttatc ccaatccaaa taagaaacga ttttttgttt aacgtcaaaa atgaaaatag
     8281 cagcctttac agagagaata acataaaaac agggaagcgc attagacggg agaattaact
     8341 gaacaccctg aacaaagtca gagggtaatt gagcgctaat atcagagaga taacccacaa
     8401 gaattgagtt aagcccaata ataagagcaa gaaacaatga aatagcaata gctatcttac
     8461 cgaagccctt tttaagaaaa gtaagcagat agccgaacaa agttaccaga aggaaaccga
     8521 ggaaacgcaa taataacgga atacccaaaa gaactggcat gattaagact ccttattacg
     8581 cagtatgtta gcaaacgtag aaaatacata cataaaggtg gcaacatata aaagaaacgc
     8641 aaagacacca cggaataagt ttattttgtc acaatcaata gaaaattcat atggtttacc
     8701 agcgccaaag acaaaagggc gacattcaac cgattgaggg agggaaggta aatattgacg
     8761 gaaattattc attaaaggtg aattatcacc gtcaccgact tgagccattt gggaattaga
     8821 gccagcaaaa tcaccagtag caccattacc attagcaagg ccggaaacgt caccaatgaa
     8881 accatcgata gcagcaccgt aatcagtagc gacagaatca agtttgcctt tagcgtcaga
     8941 ctgtagcgcg ttttcatcgg cattttcggt catagccccc ttattagcgt ttgccatctt
     9001 tt
//
