LOCUS       sequence-485111-        4948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL3 luciferase vector with 146bp PDE6B promoter (containing NRL 
            response element)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Shayler DWH, Stachelek K, Cambier L, Lee S, Bai J, Reid MW, 
            Weisenberger DJ, Bhat B, Aparicio JG, Kim Y, Singh M, Bay M, 
            Thornton ME, Doyle EK, Fouladian Z, Erberich SG, Grubbs BH, 
            Bonaguidi MA, Craft CM, Singh HP, Cobrinik D
  TITLE     Identification and characterization of early human photoreceptor 
            states and cell-state-specific retinoblastoma-related features
  JOURNAL   eLife 13:RP101918
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife 
            13:RP101918"
COMMENT     Sequence Label: pGL3-PDE6B-146
FEATURES             Location/Qualifiers
     source          1..4948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      212..221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             218..1870
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(284..302)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1911..2032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1922..1941)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1976..1995
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2280..2297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2451..3039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2531..2550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3210..4001,4002..4070))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3833..3852
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4071..4175)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4202..4657
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4289..4308)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4499..4520
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4788..4836
                     /note="synthetic polyadenylation signal"
     misc_feature    4850..4941
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4890..4909
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagagcgc aggcccccat ttgtaggagt gagtcagctg
       61 acccgccccc ggggttccta atctcactaa gaaagacttt gctgatgaca gggtttcctg
      121 ggagtccatg cgtgcctgga gcagcagcgt ctccagggac aggcagccac cgatctgcga
      181 tcaagcttgg cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa
      241 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
      301 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
      361 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
      421 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
      481 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
      541 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
      601 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
      661 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
      721 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
      781 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
      841 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
      901 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
      961 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     1021 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     1081 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     1141 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     1201 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     1261 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     1321 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     1381 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     1441 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     1501 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     1561 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     1621 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     1681 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     1741 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     1801 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     1861 cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat
     1921 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1981 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2041 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     2101 gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag
     2161 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     2221 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     2281 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2341 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2401 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2461 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2521 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2581 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2641 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2701 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2761 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2821 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2881 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2941 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3001 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3061 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3121 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3181 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3241 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3301 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3361 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3421 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3481 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3541 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3601 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3661 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3721 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3781 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3841 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3901 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3961 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4021 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4081 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4141 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4201 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4261 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4321 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4381 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4441 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4501 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4561 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4621 aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt caggctgcgc
     4681 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca
     4741 tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt
     4801 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     4861 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     4921 caggtgccag aacatttctc tatcgata
//
