LOCUS       sequence-485324-       14467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of indicator protein fusion (modified ecliptic pHluorin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14467)
  AUTHORS   Gao D, Knight MR, Trewavas AJ, Sattelmacher B, Plieth C
  TITLE     Self-reporting Arabidopsis expressing pH and [Ca2+] indicators 
            unveil ion dynamics in the cytoplasm and in the apoplast under 
            abiotic stress.
  JOURNAL   Plant Physiol. 2004 Mar;134(3):898-908. doi: 10.1104/pp.103.032508.
  PUBMED    15020753
REFERENCE   2  (bases 1 to 14467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1104/pp.103"; 
            journalName: "Plant Physiol"; date: "2004-03"; volume: "134"; issue:
            "3"; pages: "898-908"
COMMENT     Sequence Label: pBI121::chitEclipGFP:LAAQ
FEATURES             Location/Qualifiers
     source          1..14467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5749..5769
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(5934..5962)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6557..6577
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      7436..7688
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7697..7714)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7697..7713)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7706..7728)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7853..7872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8063..8084
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8331..8355
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9951..11099)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11398..12192)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12384..13151
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(13844..14467,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 ggatccaagg agatataaca atgaagacta atctttttct ctttctcatc ttttcacttc
     5881 tcctatcatt atcctcggcc gaattcagta aaggagaaga acttttcact ggagttgtcc
     5941 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     6001 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     6061 tacctgttcc atggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa
     6121 gatacccaga tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg
     6181 tgcaggagag gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca
     6241 agtttgaggg agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggaag
     6301 atggaaacat tcttggacac aaattggaat acaactataa cgatcaccag gtgtacatca
     6361 tggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattagacac aacattgaag
     6421 atggaggcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatgggcccg
     6481 tccttttacc agacaaccat tacctgttta caacttctac tctttcgaaa gatcccaacg
     6541 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     6601 tggatgaact atacaagtcg actcgactcg agcttacacc agacttcgac aacccaaaat
     6661 ggattggacg acacaagcat atgttcaatt tccttgatgt caaccacaat ggaaaaatct
     6721 ctcttgacga gatggtctac aaggcatctg atattgtcat caataacctt ggagcaacac
     6781 ctgagcaagc caaacgacac aaagatgctg tagaagcctt cttcggagga gctggaatga
     6841 aatatggtgt ggaaactgat tggcctgcat atattgaagg atggaaaaaa ttggctactg
     6901 atgaattgga gaaatacgcc aaaaacgaac caacgctcat ccgtatatgg ggtgatgctt
     6961 tgtttgatat cgttgacaaa gcccaaaatg gagccatttc actggatgaa tggaaagcat
     7021 acaccaaatc tgctggcatc atccaatcgt cagaagactg cgaggaaaca ttcagagtgt
     7081 gcgatattga tgaaagtgga caactcgatg ttgatgagat gacaagacaa catttaggat
     7141 tttggtacac catggatcct gcttgtgaaa agctctacgg tggagctgtc ccctaagaaa
     7201 ctctgctgca ggcatgcccg ctgaaatcac cagtctctct ctacaaatct atctctctct
     7261 ataataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     7321 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     7381 taaaatttct aattcctaaa accaaaatcc aggggtaccg agctcgaatt tccccgatcg
     7441 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     7501 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     7561 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     7621 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     7681 actagatcgg gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7741 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7801 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc
     7861 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7921 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7981 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8041 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8101 atctcgggct attcttttga tttataaggg attttgccga tttcggaacc accatcaaac
     8161 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     8221 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccccag
     8281 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     8341 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     8401 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     8461 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     8521 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     8581 ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca
     8641 tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat
     8701 cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac
     8761 gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc cgaagttccg
     8821 actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga gaaagcccag
     8881 taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa
     8941 acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg
     9001 gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg
     9061 ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc
     9121 gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc
     9181 gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc
     9241 catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg
     9301 cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa
     9361 gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc
     9421 cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag
     9481 cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc
     9541 cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc
     9601 gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg
     9661 ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga
     9721 caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt
     9781 aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt
     9841 cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc
     9901 agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg
     9961 caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct
    10021 tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac
    10081 atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg
    10141 aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag
    10201 tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt
    10261 ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca
    10321 acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc
    10381 ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg
    10441 aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc
    10501 agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg
    10561 acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg
    10621 aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca
    10681 atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg
    10741 gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg
    10801 gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt
    10861 tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt
    10921 tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg
    10981 aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg
    11041 gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt
    11101 caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt
    11161 gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg
    11221 tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc
    11281 agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg
    11341 cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta
    11401 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
    11461 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
    11521 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
    11581 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
    11641 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
    11701 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
    11761 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
    11821 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
    11881 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
    11941 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
    12001 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
    12061 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
    12121 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
    12181 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
    12241 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    12301 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    12361 ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag tgtatgatgg
    12421 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
    12481 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac
    12541 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag
    12601 aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg cattatgggc
    12661 gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc
    12721 gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggctatg
    12781 tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc cggaagacgg
    12841 ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc
    12901 tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc
    12961 tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc
    13021 tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg
    13081 tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg
    13141 gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa
    13201 gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc
    13261 tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat
    13321 attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc
    13381 tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa
    13441 ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc gctgggttta
    13501 tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa
    13561 aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc
    13621 cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca
    13681 cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg
    13741 ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg
    13801 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt
    13861 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
    13921 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
    13981 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
    14041 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
    14101 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
    14161 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
    14221 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
    14281 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    14341 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac
    14401 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
    14461 agcccgg
//
