LOCUS       sequence-485325-       14388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of indicator protein fusion (soluble modified ratiometric
            pHluorin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14388)
  AUTHORS   Gao D, Knight MR, Trewavas AJ, Sattelmacher B, Plieth C
  TITLE     Self-reporting Arabidopsis expressing pH and [Ca2+] indicators 
            unveil ion dynamics in the cytoplasm and in the apoplast under 
            abiotic stress.
  JOURNAL   Plant Physiol. 2004 Mar;134(3):898-908. doi: 10.1104/pp.103.032508.
  PUBMED    15020753
REFERENCE   2  (bases 1 to 14388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1104/pp.103"; 
            journalName: "Plant Physiol"; date: "2004-03"; volume: "134"; issue:
            "3"; pages: "898-908"
COMMENT     Sequence Label: pBI121::smRatioGFP:AQ
FEATURES             Location/Qualifiers
     source          1..14388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5749..5769
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(5855..5883)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6478..6498
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      7357..7609
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7618..7635)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7618..7634)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7627..7649)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7774..7793)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7984..8005
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8252..8276
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9872..11020)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11319..12113)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12305..13072
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(13765..14388,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 aacaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     5881 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     5941 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     6001 acttgtcact actttctctt atggtgttca atgcttttca agatacccag atcatatgaa
     6061 gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga ggaccatctc
     6121 tttcaaggac gacgggaact acaagacacg tgctgaagtc aagtttgagg gagacaccct
     6181 cgtcaacagg atcgagctta agggaatcga tttcaaggat gatggaaaca ttcttggaca
     6241 caaattggaa tacaactata acgagcactt ggtgtacatc atggcagaca aacaaaagaa
     6301 tggtaccaaa gctatctttc aagttcacca caacattgaa gatggaggcg ttcaactagc
     6361 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     6421 ttacctgcac acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     6481 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaagtc
     6541 gactcgactc gagcttacac cagacttcga caacccaaaa tggattggac gacacaagca
     6601 catgtttaat tttcttgatg tcaaccacaa tggaaggatc tctcttgacg agatggtcta
     6661 caaggcgtcc gatattgtta taaacaatct tggagcaaca cctgaacaag ccaaacgtca
     6721 caaagatgct gtagaagcct tcttcggagg agctggaatg aaatatggtg tagaaactga
     6781 atggcctgaa tacatcgaag gatggaaaag actggcttcc gaggaattga aaaggtattc
     6841 aaaaaaccaa atcacactta ttcgtttatg gggtgatgca ttgttcgata tcattgacaa
     6901 agaccaaaat ggagctattt cactggatga atggaaagca tacaccaaat ctgctggcat
     6961 catccaatcg tcagaagatt gcgaggaaac attcagagtg tgcgatattg acgaaagtgg
     7021 acagctcgat gttgatgaga tgacaagaca acatttagga ttttggtaca ccatggatcc
     7081 tgcttgcgaa aagctctacg gtggagctgt cccctaagaa actctgctgc aggcatgccc
     7141 gctgaaatca ccagtctctc tctacaaatc tatctctctc tataataatg tgtgagtagt
     7201 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
     7261 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     7321 aaccaaaatc caggggtacc gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     7381 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     7441 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     7501 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     7561 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattcact
     7621 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7681 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7741 ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc ccttcctttc
     7801 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7861 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     7921 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7981 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     8041 atttataagg gattttgccg atttcggaac caccatcaaa caggattttc gcctgctggg
     8101 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     8161 gctgttgccc gtctcactgg tgaaaagaaa aaccacccca gtacattaaa aacgtccgca
     8221 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     8281 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     8341 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     8401 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     8461 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc
     8521 atctccctcg cagagatccg aattatcagc cttcttattc atttctcgct taaccgtgac
     8581 aggctgtcga tcttgagaac tatgccgaca taataggaaa tcgctggata aagccgctga
     8641 ggaagctgag tggcgctatt tctttagaag tgaacgttga cgatatcaac tcccctatcc
     8701 attgctcacc gaatggtaca ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc
     8761 cccggctgat cgaccccaga tctggggctg agaaagccca gtaaggaaac aactgtaggt
     8821 tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc gatcaggccg
     8881 agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg atcaaacact
     8941 aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt aaaggtgagc
     9001 agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc
     9061 cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt caacaccaac
     9121 agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta
     9181 cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc taccgtcgcc
     9241 gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata
     9301 ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggacg
     9361 atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc gacccctcgg
     9421 cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg
     9481 ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta ggcgccgaat
     9541 gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg
     9601 taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat ctcgcggaaa
     9661 tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat tgtcaatttt
     9721 aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga
     9781 agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg
     9841 aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat
     9901 tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct
     9961 gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca
    10021 gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg
    10081 gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca
    10141 cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga
    10201 cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc
    10261 ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat
    10321 tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga
    10381 taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc
    10441 gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt
    10501 tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg
    10561 tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct
    10621 gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga
    10681 cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg
    10741 tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct
    10801 ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga
    10861 tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac
    10921 gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca
    10981 gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg
    11041 ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg
    11101 tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct
    11161 tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat
    11221 acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct
    11281 cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc atccagtaaa
    11341 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
    11401 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
    11461 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
    11521 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
    11581 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    11641 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    11701 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    11761 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    11821 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    11881 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    11941 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    12001 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    12061 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    12121 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    12181 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    12241 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat
    12301 tatgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
    12361 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
    12421 gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac
    12481 tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca
    12541 tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc aacacgattt
    12601 tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga
    12661 cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta aatcgcgcca
    12721 gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc acgtattcgg
    12781 tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt
    12841 gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt
    12901 aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga ggcagcacct
    12961 ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt
    13021 catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta cccaattatg
    13081 atagaattta caagctataa ggttattgtc ctgggtttca agcattagtc catgcaagtt
    13141 tttatgcttt gcccattcta tagatatatt gataagcgcg ctgcctatgc cttgccccct
    13201 gaaatcctta catacggcga tatcttctat ataaaagata tattatctta tcagtattgt
    13261 caatatattc aaggcaatct gcctcctcat cctcttcatc ctcttcgtct tggtagcttt
    13321 ttaaatatgg cgcttcatag agtaattctg taaaggtcca attctcgttt tcatacctcg
    13381 gtataatctt acctatcacc tcaaatggtt cgctgggttt atcgcacccc cgaacacgag
    13441 cacggcaccc gcgaccacta tgccaagaat gcccaaggta aaaattgccg gccccgccat
    13501 gaagtccgtg aatgccccga cggccgaagt gaagggcagg ccgccaccca ggccgccgcc
    13561 ctcactgccc ggcacctggt cgctgaatgt cgatgccagc acctgcggca cgtcaatgct
    13621 tccgggcgtc gcgctcgggc tgatcgccca tcccgttact gccccgatcc cggcaatggc
    13681 aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg ggcgcagccc
    13741 ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc
    13801 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
    13861 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
    13921 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
    13981 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
    14041 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
    14101 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
    14161 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
    14221 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
    14281 cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
    14341 cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccgg
//
