LOCUS       sequence-485471-        8862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This virus-induced gene silencing (VIGS) reporter vector is used to 
            evaluate the silencing of GhOMT1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 8862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVSr-GhOMT1
FEATURES             Location/Qualifiers
     source          1..8862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        618..963
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     906..926
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             982..1917
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1972..2148
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     2297..2319
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2312..2328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2338..2357
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2338..2356
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2381..2397
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2382..2398
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2407..4116
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          3155..4116
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     CDS             4954..6606
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5020..5038)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    6668..6844
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6854..6878
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6969..7557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7049..7068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7728..8543)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7728..8543)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8451..8470
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8535..8554
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(8834..8862,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt atcgtacccc tactccaaaa atgtcaaaga tacagtctca
      601 gaagaccaaa gggctattga gacttttcaa caaagggtaa tttcgggaaa cctcctcgga
      661 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      721 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatgcctc tgccgacagt
      781 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
      841 acgtcttcaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa
      901 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      961 acagcccaag ctctagccac catgacttcg aaagtttatg atccagaaca aaggaaacgg
     1021 atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt
     1081 attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac
     1141 gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt
     1201 attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg
     1261 ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag
     1321 atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat
     1381 caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg
     1441 gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa
     1501 atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag
     1561 ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt
     1621 cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt
     1681 gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg
     1741 tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt
     1801 cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
     1861 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc
     1921 tagggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta ttttctccat
     1981 aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg gtttcgctca
     2041 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     2101 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatcga taacattaac
     2161 gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2221 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2281 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     2341 tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat
     2401 aagcttattc gggtcaaggc ggaagccagc gcgccacccc acgtcagcaa atacggaggc
     2461 gcggggttga cggcgtcacc cggtcctaac ggcgaccaac aaaccagcca gaagaaatta
     2521 cagtaaaaaa aaagtaaatt gcactttgat ccacctttta ttacctaagt ctcaatttgg
     2581 atcaccctta aacctatctt ttcaatttgg gccgggttgt ggtttggact accatgaaca
     2641 acttttcgtc atgtctaact tccctttcag caaacatatg aaccatatat agaggagatc
     2701 ggccgtatac tagagctgat gtgtttaagg tcgttgattg cacgagaaaa aaaaatccaa
     2761 atcgcaacaa tagcaaattt atctggttca aagtgaaaag atatgtttaa aggtagtcca
     2821 aagtaaaact tatagataat aaaatgtggt ccaaagcgta attcactcaa aaaaaatcaa
     2881 cgagacgtgt accaaacgga gacaaacggc atcttctcga aatttcccaa ccgctcgctc
     2941 gcccgcctcg tcttcccgga aaccgcggtg gtttcagcgt ggcggattct ccaagcagac
     3001 ggagacgtca cggcacggga ctcctcccac cacccaaccg ccataaatac cagccccctc
     3061 atctcctctc ctcgcatcag ctccaccccc gaaaaatttc tccccaatct cgcgaggctc
     3121 tcgtcgtcga atcgaatcct ctcgcgtcct caaggtacgc tgcttctcct ctcctcgctt
     3181 cgtttcgatt cgatttcgga cgggtgaggt tgttttgttg ctagatccga ttggtggtta
     3241 gggttgtcga tgtgattatc gtgagatgtt taggggttgt agatctgatg gttgtgattt
     3301 gggcacggtt ggttcgatag gtggaatcgt ggttaggttt tgggattgga tgttggttct
     3361 gatgattggg gggaattttt acggttagat gaattgttgg atgattcgat tggggaaatc
     3421 ggtgtagatc tgttggggaa ttgtggaact agtcatgcct gagtgattgg tgcgatttgt
     3481 agcgtgttcc atcttgtagg ccttgttgcg agcatgttca gatctactgt tccgctcttg
     3541 attgagttat tggtgccatg ggttggtgca aacacaggct ttaatatgtt atatctgttt
     3601 tgtgtttgat gtagatctgt agggtagttc ttcttagaca tggttcaatt atgtagcttg
     3661 tgcgtttcga tttgatttca tatgttcaca gattagataa tgatgaactc ttttaattaa
     3721 ttgtcaatgg taaataggaa gtcttgtcgc tatatctgtc ataatgatct catgttacta
     3781 tctgccagta atttatgcta agaactatat tagaatatca tgttacaatc tgtagtaata
     3841 tcatgttaca atctgtagtt catctatata atctattgtg gtaatttctt tttactatct
     3901 gtgtgaagat tattgccact agttcattct acttatttct gaagttcagg atacgtgtgc
     3961 tgttactacc tatctgaata catgtgtgat gtgcctgtta ctatcttttt gaatacatgt
     4021 atgttctgtt ggaatatgtt tgctgtttga tccgttgttg tgtccttaat cttgtgctag
     4081 ttcttaccct atctgtttgg tgattatttc ttgcagatag ttatcggatc catccggggg
     4141 gcaatgagat ccgagaccat gcagataagg tctcaaaatt ttggtaaagc gaaacaacca
     4201 gaaacaggcg ctgagaaaga aaccatgtct caagaagatc aagaggaaga agttgggaaa
     4261 ctggccgtcc gcctagccaa cgccgtggta cttccaatgg tcttgaaatc agccttggag
     4321 ctgaacataa ttgacacaat cttagccgct ggtgacggcg cgtttctgtc accttcccag
     4381 attgcgagtg cccttccttc aaagaatcct gacgcaccag tgctactaga tcgaatgcta
     4441 cgcctgttgg ccagccattc cattctcaaa tgcgcagtaa aagcaaagga aaaagaagaa
     4501 attgaaagac tgtacggtgc aggcccacta tgcaagttcc ttgttaagaa tcaagatgga
     4561 gggtcgattg cacctctcct tttgttgcac catgaccaag tcttcatgca aagctggtac
     4621 catttaaatg atgctatact agaaggaggg gttcctttta gtaaggcata cgggatgaca
     4681 gcatttgaat atccaggaac tgatcaacga ttcaatagag tatttaacca ggcaatgtca
     4741 aatcatactg ctttgataat gaggaagatt gttgatgttt acaaagggtt tgatgggttg
     4801 aaagtgttgg ttgatgtggg tggtgggatt ggggttgctc tcagttttat tacttcaaag
     4861 tatcctcaaa tcaagggcat caactttgat ctgcctcatg ttttggctga tgcacccact
     4921 tattcaggtc tcatggtgag caagggcgag gagatggaag acgccaaaaa cataaagaaa
     4981 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct
     5041 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
     5101 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga
     5161 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt
     5221 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat
     5281 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc
     5341 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt
     5401 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca
     5461 tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac
     5521 aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct
     5581 ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat
     5641 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg
     5701 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa
     5761 gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca
     5821 accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta
     5881 cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag
     5941 aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt
     6001 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt
     6061 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa
     6121 ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac
     6181 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac
     6241 gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg
     6301 gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc
     6361 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac
     6421 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg
     6481 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa
     6541 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc
     6601 gtgtaattct agagaattcg gtacgctgaa atcaccagtc tctctctaca aatctatctc
     6661 tctctatttt ctccataaat aatgtgtgag tagtttcccg ataagggaaa ttagggttct
     6721 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     6781 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     6841 gatccactag ccttgacagg atatattggc gggtaaacta agtcgctgta tgtgtttgtt
     6901 tgagatctca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6961 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7021 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7081 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7141 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7201 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7261 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7321 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7381 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7441 ccagttacct tcggaagaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7501 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7561 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7621 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7681 agttttaaat caatctaaag tatatatgtg taacattggt ctagtgatta gaaaaactca
     7741 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     7801 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     7861 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     7921 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     7981 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     8041 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     8101 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     8161 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     8221 tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     8281 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     8341 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     8401 tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc
     8461 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac
     8521 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
     8581 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
     8641 acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat
     8701 cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc
     8761 acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg
     8821 attcaggcga tccccatcca acagcccgcc gtcgagcggg ct
//
