LOCUS       sequence-485602-        9854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ZZ-3xTEV-HsATG14 and ZZ-3xTEV-HsBECN1 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O,
            Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi 
            M, Ktistakis NT, Sachse C, Williams RL
  TITLE     Characterization of Atg38 and NRBF2, a fifth subunit of the 
            autophagic Vps34/PIK3C3 complex.
  JOURNAL   Autophagy. 2016 Nov;12(11):2129-2144. doi: 
            10.1080/15548627.2016.1226736. Epub 2016 Sep 14.
  PUBMED    27630019
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2016.1226736"; journalName: "Autophagy"; date: 
            "2016-11"; volume: "12"; issue: "11"; pages: "2129-2144"
COMMENT     Sequence Label: pYO1022
FEATURES             Location/Qualifiers
     source          1..9854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..585
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     536..556
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    587..605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    608..626
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     636..660
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             783..803
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             819..992
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             993..1166
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1176..1196
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1227..1247
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1269..1289
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(2880..2897)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2886..3110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3167..3470
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3472..3749
                     /label=chicken beta-actin promoter
     intron          3750..4766
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4690..4712
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4774..4793
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4873..4893
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4909..5082
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             5083..5256
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             5266..5286
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5317..5337
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5359..5379
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(6876..6895)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6941..6996
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6995..7014)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7357..7373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7357..7373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7370..7392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7381..7397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7405..7411,7412..7429,7430..7435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7450..7471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7530..7725
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7576..7711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7731..7865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7768..7787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7822..7841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7933..7950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8104..8692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8184..8203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8863..9654,9655..9723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9486..9505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9724..9828)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
      601 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      661 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      721 ctccggactc tagcgtttaa acttaagctt ggtaccggat gggagcaggg gcgggtgcta
      781 gcgagaacct gtacttccag ggcgcccacg atgaagccgt agacaacaaa ttcaacaaag
      841 aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa gaacaacgaa
      901 acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt ttagcagaag
      961 ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac aaagaacaac
     1021 aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct
     1081 tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa
     1141 agctaaatga tgctcaggcg ccgaaagtag acgcggaaaa cctgtatttc cagggaggcg
     1201 gatcttctag accaactact gcctctgaaa atttgtactt tcaaggcgag ttgaagacca
     1261 ctgcttctga aaacttgtac ttccaaggtg agttactcga gggtgaattg aagactgctg
     1321 ctttggctca acacgacgaa gccatgacgg cgtctcccag tgggaaggga gcccgggcgc
     1381 tggaggctcc tggctgcggg ccccggccgc tcgcccggga cctggtggac tccgtggacg
     1441 atgcggaggg gctgtacgtg gctgtggagc gctgcccgct gtgcaacact acccgccggc
     1501 ggctgacctg cgccaaatgc gttcagagcg gcgatttcgt ctacttcgac ggccgcgacc
     1561 gggagaggtt tatcgacaag aaggaaaggt taagccgact taagagcaag caagaagaat
     1621 ttcagaaaga agtgttaaaa gctatggaag gaaaatggat aacagatcag ttgagatgga
     1681 aaataatgtc ctgcaagatg aggattgaac agttaaaaca aacaatatgt aaaggaaatg
     1741 aagaaatgga gaaaaattct gaaggccttc tcaaaaccaa ggaaaagaat cagaagcttt
     1801 acagtcgagc acaacggcac caagagaaaa aggagaagat tcagaggcat aatcgcaaac
     1861 ttggtgacct ggtagaaaaa aagaccattg acttaagaag tcattatgag cgtctggcaa
     1921 atcttcgacg atcccatata ttagagctca cctctgtcat ttttccaatc gaggaagtaa
     1981 agacgggtgt gagagacccc gcagatgtgt cttcagagag tgacagtgcc atgacctcca
     2041 gcactgtgag caagcttgct gaagcccgga ggacaactta cctctcagga cgatgggtct
     2101 gtgacgatca cagcggagac accagcatta gcattacagg gccttggatt agcctcccta
     2161 acaatgggga ctactctgcc tactacagct gggtggagga gaagaaaaca acccaggggc
     2221 ctgacatgga gcagagtaac cctgcctaca ccatcagtgc tgcgctgtgc tatgcaactc
     2281 agctggtcaa cattctgtct catatacttg atgtaaatct tcccaaaaag ctctgcaaca
     2341 gtgaattttg tggcgaaaat ctaagcaagc agaaatttac tcgagcagtg aagaaactga
     2401 atgcaaatat tctttacctt tgtttttctc agcatgtaaa tttagatcaa ttacaaccac
     2461 tgcataccct caggaatcta atgtacctgg tcagtccaag ctctgaacac ctaggcaggt
     2521 cagggccctt tgaagtacga gcagaccttg aggagtccat ggaatttgtg gatcccggag
     2581 ttgctggaga atcagatgag agcggagatg agcgcgtcag cgatgaagaa accgacctgg
     2641 gcacagactg ggagaacttg cctagtcccc ggttttgtga tatcccttcc cagtctgtgg
     2701 aagtctccca gagtcagagc acccaggcgt ccccacccat cgcgagcagc agtgcaggtg
     2761 ggatgatctc ctctgcagca gcctcggtga cctcctggtt taaagcttac actggacacc
     2821 gtagatccta acccaagctt aagtcgagtc tagagggccc gtttaaaccc gctgatcagc
     2881 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     2941 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     3001 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     3061 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg tattaatagt
     3121 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     3181 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     3241 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     3301 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     3361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     3421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     3481 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     3541 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc
     3601 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
     3661 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     3721 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
     3781 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     3841 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     3901 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     3961 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     4021 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     4081 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     4141 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     4201 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     4261 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     4321 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     4381 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     4441 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     4501 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     4561 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     4621 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     4681 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     4741 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     4801 atcattttgg caaagaattg ctcgatcgac ggtatcgatg ccaccatgat gggagcaggg
     4861 gcgggtgcta gcgagaacct gtacttccag ggcgcccacg atgaagccgt agacaacaaa
     4921 ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa
     4981 gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt
     5041 ttagcagaag ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac
     5101 aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa
     5161 cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca
     5221 gaagctaaaa agctaaatga tgctcaggcg ccgaaagtag acgcggaaaa cctgtatttc
     5281 cagggaggcg gatcttctag accaactact gcctctgaaa atttgtactt tcaaggcgag
     5341 ttgaagacca ctgcttctga aaacttgtac ttccaaggtg agttactcga gggtgaattg
     5401 aagactgctg ctttggctca acacgacgaa gccatggaag ggtctaagac gtccaacaac
     5461 agcaccatgc aggtgagctt cgtgtgccag cgctgcagcc agcccctgaa actggacacg
     5521 agtttcaaga tcctggaccg tgtcaccatc caggaactca cagctccatt acttaccaca
     5581 gcccaggcga aaccaggaga gacccaggag gaagagacta actcaggaga ggagccattt
     5641 attgaaactc ctcgccagga tggtgtctct cgcagattca tccccccagc caggatgatg
     5701 tccacagaaa gtgccaacag cttcactctg attggggagg catctgatgg cggcaccatg
     5761 gagaacctca gccgaagact gaaggtcact ggggaccttt ttgacatcat gtcgggccag
     5821 acagatgtgg atcacccact ctgtgaggaa tgcacagata ctcttttaga ccagctggac
     5881 actcagctca acgtcactga aaatgagtgt cagaactaca aacgctgttt ggagatctta
     5941 gagcaaatga atgaggatga cagtgaacag ttacagatgg agctaaagga gctggcacta
     6001 gaggaggaga ggctgatcca ggagctggaa gacgtggaaa agaaccgcaa gatagtggca
     6061 gaaaatctcg agaaggtcca ggctgaggct gagagactgg atcaggagga agctcagtat
     6121 cagagagaat acagtgaatt taaacgacag cagctggagc tggatgatga gctgaagagt
     6181 gttgaaaacc agatgcgtta tgcccagacg cagctggata agctgaagaa aaccaacgtc
     6241 tttaatgcaa ccttccacat ctggcacagt ggacagtttg gcacaatcaa taacttcagg
     6301 ctgggtcgcc tgcccagtgt tcccgtggaa tggaatgaga ttaatgctgc ttggggccag
     6361 actgtgttgc tgctccatgc tctggccaat aagatgggtc tgaaatttca gagataccga
     6421 cttgttcctt acggaaacca ttcatatctg gagtctctga cagacaaatc taaggagctg
     6481 ccgttatact gttctggggg gttgcggttt ttctgggaca acaagtttga ccatgcaatg
     6541 gtggctttcc tggactgtgt gcagcagttc aaagaagagg ttgagaaagg cgagacacgt
     6601 ttttgtcttc cctacaggat ggatgtggag aaaggcaaga ttgaagacac aggaggcagt
     6661 ggcggctcct attccatcaa aacccagttt aactctgagg agcagtggac aaaagctctc
     6721 aagttcatgc tgacgaatct gaagtggggt ctggcttggg tgtcctctca attttataac
     6781 aaatgactcg aggaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     6841 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     6901 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     6961 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     7021 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     7081 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     7141 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     7201 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     7261 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     7321 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7381 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7441 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7501 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     7561 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7621 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7681 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     7741 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7801 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7861 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7921 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7981 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8041 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8101 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8161 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8221 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8281 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8341 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8401 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8461 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8521 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8581 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8641 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8701 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8761 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8821 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8881 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8941 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9001 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9061 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9121 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9181 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9241 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9301 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9361 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9421 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9481 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9541 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9601 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9661 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9721 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9781 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9841 aaaagtgcca cctg
//
