LOCUS       sequence-485607-        9677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressed mCherry2 florescent proteins in plant cells for tracing 
            transformation events.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Liu MS, Huang TK, Wang YC, Wang SC, Wu CH, Kuo CH, Lai EM
  TITLE     Floral stage optimization and immune evasion enhance 
            Agrobacterium-mediated genome editing in Arabidopsis.
  JOURNAL   New Phytol. 2025 Nov 30. doi: 10.1111/nph.70795.
  PUBMED    41321035
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2025 Nov 30. doi: 10.1111/nph.70795."
COMMENT     Sequence Label: EML6442_p35S-mCherry2-SV40
FEATURES             Location/Qualifiers
     source          1..9677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..60
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             369..998
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1427..2500
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2566..2760
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        2968..3072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3073..3867
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4038..4626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4527..4546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4762..4786
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4949..5128
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5159..5213
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5215..6009
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5269..5288)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5879..5898
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6036..6743
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        7755..8100
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8043..8063
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8902..8922
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8939..9646
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
       61 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      121 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtcagccgtg
      181 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
      241 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
      301 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
      361 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
      421 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
      481 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
      541 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
      601 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
      661 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
      721 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
      781 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
      841 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
      901 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
      961 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     1021 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     1081 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     1141 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     1201 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     1261 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     1321 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     1381 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     1441 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     1501 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     1561 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgtc
     1621 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     1681 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     1741 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     1801 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     1861 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     1921 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     1981 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     2041 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     2101 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     2161 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     2221 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     2281 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     2341 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     2401 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     2461 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     2521 gcaaattgcc ctagcagggg aaaaaggtcg aaaaagcttc tttcctgtgg atagcacgta
     2581 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     2641 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     2701 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     2761 actgtctggc cagcgcacag ccgaacagct gcaaaaagcg cccacatcaa ggctccgagt
     2821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2881 acaataaccc ksmtrrmykg tgcataactg tctggccagc gcacagccga acagctgcaa
     2941 aaagcgccca catcaaggct ccgagtgcgc ggaaccccta tttgtttatt tttctaaata
     3001 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3061 aaaaggaaga gtatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
     3121 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
     3181 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
     3241 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
     3301 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
     3361 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
     3421 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
     3481 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
     3541 gaaaactggg aagaggacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
     3601 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
     3661 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
     3721 aagtggtatg acattgcctt ctgcgtccgg tcgctcaggg aggatatcgg ggaagaacag
     3781 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
     3841 tatattttac tggatgaatt gttttagctg tcagaccaag tttactcata tatactttag
     3901 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3961 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgc tcggatctgt tggaccggac
     4681 agtagtcatg gttgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
     4741 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
     4801 acaacttaat aacacattgc ggacgttttt aatgtactgg ggttgaacac tctgtgccga
     4861 attcggatcc ggagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
     4921 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actgagccgc
     4981 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
     5041 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
     5101 tccataaatt cccctcggta tccaattaga gtctcatatt cactctccta tttttacaac
     5161 aattaccaac aacaacaaac aacaaacaac attacaatta catttacaat taccatggtt
     5221 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     5281 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5341 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     5401 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5461 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5521 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5581 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5641 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5701 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     5761 gatctcgtcg tgactcatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5821 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5881 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5941 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6001 ttcttctgag cgggactctg gggttcgcta gagtcctgct ttaatgagat atgcgagacg
     6061 cctatgatcg catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca
     6121 tgtgtagctc agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta
     6181 ctatcgtatt tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta
     6241 tatgtacaat attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta
     6301 tgatagagcg ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa
     6361 aagcggcaga accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa
     6421 agtgccccag gggctagtat ctacgacaca ccgagcggcg aactaataac gctcactgaa
     6481 gggaactccg gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc
     6541 gtccgctcta ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa
     6601 ccgacttgct gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa
     6661 gtgcaggtca aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc
     6721 gacaacatgt cgaggctcag ccgctgcaag aattcaagct tggaggaatt ccaatcccac
     6781 aaaaatctga gcttaacagc acagttgctc ctctcagagc agaatcgggt attcaacacc
     6841 ctcatatcaa ctactacgtt gtgtataacg gtccacatgc cggtatatac gatgactggg
     6901 gttgtacaaa ggcggcaaca aacggcgttc ccggagttgc acacaagaaa tttgccacta
     6961 ttacagaggc aagagcagca gctgacgcgt acacaacaag tcagcaaaca gacaggttga
     7021 acttcatccc caaaggagaa gctcaactca agcccaagag ctttgctaag gccctaacaa
     7081 gcccaccaaa gcaaaaagcc cactggctca cgctaggaac caaaaggccc agcagtgatc
     7141 cagccccaaa agagatctcc tttgccccgg agattacaat ggacgatttc ctctatcttt
     7201 acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc actgataatg
     7261 agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct
     7321 acgcagcaag tctcatcaag acgatctacc cgagtaacaa tctccaggag atcaaatacc
     7381 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc aagaacacag
     7441 agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
     7501 ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cctactgaat
     7561 ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact cgccgtcaag
     7621 actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc
     7681 aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa
     7741 gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc
     7801 cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac
     7861 aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt
     7921 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg
     7981 tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc
     8041 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
     8101 cgctcgagta taagagctca tttttacaac aattaccaac aacaacaaac aacaaacaac
     8161 attacaatta catttacaat tatcgataca atggtgagta aaggagaaga aaacaactta
     8221 gctatcatta aagagttcat gcgcttcaaa gttcacatgg agggttctgt taacggtcac
     8281 gagttcgaga tcgaaggcga aggcgagggc cgtccgtatg aaggcaccca gaccgccaaa
     8341 ctgaaagtga ctaaaggcgg cccgctgcct tttgcgtggg acatcctgag cccgcaattt
     8401 atgtacggtt ctaaagcgta tgttaaacac ccagcggata tcccggacta tctgaagctg
     8461 tcttttccgg aaggtttcaa ctgggaacgc gtaatgaatt ttgaagatgg tggtgtcgtg
     8521 accgtcactc aggactcctc cctgcaggat ggcgagttca tctataaagt taaactgcgt
     8581 ggtactaatt ttccatctga tggcccggtg atgcagtgta ggacgatggg ttgggaggcg
     8641 tctaccgaac gcatgtatcc ggaagatggt gcgctgaaag gcgaaattaa acagcgcctg
     8701 aaactgaaag atggcggcca ttatgacgct gaagtgaaaa ccacgtacaa agccaagaaa
     8761 cctgtgcagc tgcctggcgc gtacaatgtg gatattaaac tggacatctt atctcataat
     8821 gaagattata cgatcgtaga gcaatatgag cgcgcggagg gtcgtcattc taccggtggc
     8881 atggatgaac tatacaaagg cccaaaaaag aagagaaagg taggcgccta agcttgtcct
     8941 gctttaatga gatatgcgag aagcctatga tcgcatgata tttgctttca attctgttgt
     9001 gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat
     9061 tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt
     9121 tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat atattgtgct
     9181 gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat tgcgttttat
     9241 tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa gactgattac
     9301 ataaatctta ttcaaatttc aaaagtgccc caggggctag tatctacgac acaccgagcg
     9361 gcgaactaat aacgctcact gaagggaact ccggttcccc gccggcgcgc atgggtgaga
     9421 ttccttgaag ttgagtattg gccgtccgct ctaccgaaag ttacgggcac cattcaaccc
     9481 ggtccagcac ggcggccggg taaccgactt gctgccccga gaattatgca gcattttttt
     9541 ggtgtatgtg ggccccaaat gaagtgcagg tcaaaccttg acagtgacga caaatcgttg
     9601 ggcgggtcca gggcgaattt tgcgacaaca tgtcgaggct cagcaggacc gctactagag
     9661 gatgcacatg tgaccga
//
