LOCUS       sequence-485632-        8867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human beta-catenin ∆C terminal IDR (deletion of a.a. 
            665-781) using piggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, 
            Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, 
            Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, 
            Cermakova K, Hodges HC
  TITLE     beta-catenin functions as a molecular adapter for disordered cBAF 
            interactions.
  JOURNAL   Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026.
  PUBMED    40695292
REFERENCE   2  (bases 1 to 8867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026."
COMMENT     Sequence Label: pPB-CAG-CTNNB1-deltaC-HA
FEATURES             Location/Qualifiers
     source          1..8867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     343..362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(581..685)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(711..1166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(848..869)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1060..1079
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1293..1315
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1307..1324
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1308..1324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1334..1353
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1334..1352
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1382..1416
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1737..2116
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2118..2394
                     /label=chicken beta-actin promoter
     primer_bind     3335..3357
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      3448..3457
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3499..3534
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     CDS             5455..5544
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    5639..6188
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5783..5800)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6010..6029
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6659..6676)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6665..6889
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6936..6952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6960..6976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6984..6990,6991..7008,7009..7014))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7029..7050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7284..7346
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7387..7407)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7387..7405)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7426..7442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7450..7466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7474..7480,7481..7498,7499..7504))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7519..7540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7657..7674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7828..8416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7908..7927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8587..8867,1..511,512..580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
       61 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
      121 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
      181 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
      241 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
      301 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
      361 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
      421 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
      481 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
      541 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
      601 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
      661 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
      721 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
      781 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      841 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      901 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      961 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     1021 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     1081 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     1141 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     1201 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1261 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1321 cagtgagcgc gcgtaatacg actcactata gggcgaattg gggcgcgcca ttctagatta
     1381 accctagaaa gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct
     1441 ttctaaatag cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct
     1501 cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg
     1561 tgagtcaaaa tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata
     1621 attatattgt tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt
     1681 tatagatatc aactagaatg ctagactcga gaagcttgat cggccacgcg gatctcgaca
     1741 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1801 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1861 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1921 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1981 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2041 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2101 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     2161 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttggg gggggggggg
     2221 gggggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2281 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2341 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2401 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     2461 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     2521 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     2581 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     2641 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     2701 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     2761 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     2821 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     2881 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     2941 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     3001 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     3061 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     3121 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     3181 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     3241 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     3301 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     3361 ggctctagcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct
     3421 ctccccaggg ggatccaccg ggctagcgcc accatggcta ctcaagctga tttgatggag
     3481 ttggacatgg ccatggaacc agacagaaaa gcggctgtta gtcactggca gcaacagtct
     3541 tacctggact ctggaatcca ttctggtgcc actaccacag ctccttctct gagtggtaaa
     3601 ggcaatcctg aggaagagga tgtggatacc tcccaagtcc tgtatgagtg ggaacaggga
     3661 ttttctcagt ccttcactca agaacaagta gctgatattg atggacagta tgcaatgact
     3721 cgagctcaga gggtacgagc tgctatgttc cctgagacat tagatgaggg catgcagatc
     3781 ccatctacac agtttgatgc tgctcatccc actaatgtcc agcgtttggc tgaaccatca
     3841 cagatgctga aacatgcagt tgtaaacttg attaactatc aagatgatgc agaacttgcc
     3901 acacgtgcaa tccctgaact gacaaaactg ctaaatgacg aggaccaggt ggtggttaat
     3961 aaggctgcag ttatggtcca tcagctttct aaaaaggaag cttccagaca cgctatcatg
     4021 cgttctcctc agatggtgtc tgctattgta cgtaccatgc agaatacaaa tgatgtagaa
     4081 acagctcgtt gtaccgctgg gaccttgcat aacctttccc atcatcgtga gggcttactg
     4141 gccatcttta agtctggagg cattcctgcc ctggtgaaaa tgcttggttc accagtggat
     4201 tctgtgttgt tttatgccat tacaactctc cacaaccttt tattacatca agaaggagct
     4261 aaaatggcag tgcgtttagc tggtgggctg cagaaaatgg ttgccttgct caacaaaaca
     4321 aatgttaaat tcttggctat tacgacagac tgccttcaaa ttttagctta tggcaaccaa
     4381 gaaagcaagc tcatcatact ggctagtggt ggaccccaag ctttagtaaa tataatgagg
     4441 acctatactt acgaaaaact actgtggacc acaagcagag tgctgaaggt gctatctgtc
     4501 tgctctagta ataagccggc tattgtagaa gctggtggaa tgcaagcttt aggacttcac
     4561 ctgacagatc caagtcaacg tcttgttcag aactgtcttt ggactctcag gaatctttca
     4621 gatgctgcaa ctaaacagga agggatggaa ggtctccttg ggactcttgt tcagcttctg
     4681 ggttcagatg atataaatgt ggtcacctgt gcagctggaa ttctttctaa cctcacttgc
     4741 aataattata agaacaagat gatggtctgc caagtgggtg gtatagaggc tcttgtgcgt
     4801 actgtccttc gggctggtga cagggaagac atcactgagc ctgccatctg tgctcttcgt
     4861 catctgacca gccgacacca agaagcagag atggcccaga atgcagttcg ccttcactat
     4921 ggactaccag ttgtggttaa gctcttacac ccaccatccc actggcctct gataaaggct
     4981 actgttggat tgattcgaaa tcttgccctt tgtcccgcaa atcatgcacc tttgcgtgag
     5041 cagggtgcca ttccacgact agttcagttg cttgttcgtg cacatcagga tacccagcgc
     5101 cgtacgtcca tgggtgggac acagcagcaa tttgtggagg gggtccgcat ggaagaaata
     5161 gttgaaggtt gtaccggagc ccttcacatc ctagctcggg atgttcacaa ccgaattgtt
     5221 atcagaggac taaataccat tccattgttt gtgcagctgc tttattctcc cattgaaaac
     5281 atccaaagag tagctgcagg ggtcctctgt gaacttgctc aggacaagga agctgcagaa
     5341 gctattgaag ctgagggagc cacagctcct ctgacagagt tacttcactc taggaatgaa
     5401 ggtgtggcga catatgcagc tgctgttttg ttccgaatgt ctgagaccgg tatgtaccca
     5461 tacgatgttc cagattacgc tggctatccc tatgacgtcc cggactatgc aggctcctat
     5521 ccatatgacg ttccagatta cgcttgaacg cgttagtgcg gatcctagtg cgaattctag
     5581 tgcgtcgact ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac
     5641 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5701 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5761 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5821 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     5881 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     5941 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     6001 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     6061 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     6121 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     6181 cgataatacc atggtcatga aaacatttaa catttctcaa caagatctag aattagtaga
     6241 agtagcgaca gagaagatta caatgcttta tgaggataat aaacatcatg tgggagcggc
     6301 aattcgtacg aaaacaggag aaatcatttc ggcagtacat attgaagcgt atataggacg
     6361 agtaactgtt tgtgcagaag ccattgcgat tggtagtgca gtttcgaatg gacaaaagga
     6421 ttttgacacg attgtagctg ttagacaccc ttattctgac gaagtagata gaagtattcg
     6481 agtggtaagt ccttgtggta tgtgtaggga gttgatttca gactatgcac cagattgttt
     6541 tgtgttaata gaaatgaatg gcaagttagt caaaactacg attgaagaac tcattccact
     6601 caaatatacc cgaaattaaa agtcgagatg catcgatgat ctagagctcg ctgatcagcc
     6661 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6721 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6781 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6841 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6901 gaaagaacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6961 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7021 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7081 tcgggaaacc tgtcgtgcca gcggatctta aaagttttgt tactttatag aagaaatttt
     7141 gagtttttgt ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt
     7201 attagtatgt aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc
     7261 tcgatataca gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac
     7321 gtcacaatat gattatcttt ctagggttaa tctagtatcg tagattaatt aatccagctt
     7381 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     7441 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7501 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7561 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgc
//
